US4769330A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Modified vaccinia virus and methods for making and using the same
Assignee: Health Research Inc
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Filing: 1981-12-24
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Granted: 1988-09-06
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Expires: 2001-12-24
Abstract
Abstract What are disclosed are methods for modifying the genome of vaccinia virus to produce vaccinia mutants, particularly by the introduction into the vaccinia genome of exogenous DNA; modified vaccinia prepared by such methods; certain DNA sequences and unmodified and genetically modified microorganisms involved as intermediates in such methods; and methods for infecting cells and host animals with such vaccinia mutants to provoke the amplification of exogenous DNA and proteins encoded by the exogenous DNA, including antigenic proteins, by said cells and host animals.
Inventors
- 👤 Enzo Paoletti
- 👤 Dennis Panicali
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 01:45
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; C12; C12N; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16611; C12N2710/16622; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16111; C12N2760/16122; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
89 words
A method for the in vivo recombination of vaccinia virus DNA with DNA not naturally occurring in vaccinia virus, which method comprises infecting a cell with vaccinia virus in a cell-compatible medium in the presence of doner DNA, said donor DNA comprising said DNA not naturally occurring in vaccinia virus flanked by DNA sequences homologous with portions of the vaccinia genome, whereby said DNA not naturally occurring in vaccinia is introduced into the genome of said vaccinia virus, and then recovering vaccinia virus modified by such in vivo recombination.
Claim 2
Independent Claim
89 words
A method for the in vivo recombination of vaccinia virus DNA with DNA not naturally occurring in vaccinia virus, which method comprises infecting a cell with vaccinia virus in a cell-compatible medium in the presence of doner DNA, said donor DNA comprising said DNA not naturally occurring in vaccinia virus flanked by DNA sequences homologous with portions of the vaccinia genome, whereby said DNA not naturally occurring in vaccinia is introduced into the genome of said vaccinia virus, and then recovering vaccinia virus modified by such in vivo recombination.
Claim 3
Dependent Claim
35 words
A method as in claim 1 wherein said flanking DNA sequences are co-linear with portions of the genome of the vaccinia virus except for the presence of said DNA not naturally occurring in vaccinia virus.
Claim 4
Dependent Claim
39 words
A method as in claim 1 wherein said flanking sequences are co-linear with portions of the genome of the vaccinia virus in a non-essential region thereof, except for the presence of said DNA not naturally occurring in vaccinia virus.
Claim 5
Independent Claim
85 words
A method for the in vivo recombination of vaccinia virus DNA and DNA not naturally occurring in vaccinia virus, which method comprises infecting a cell with vaccinia virus in a cell-compatible medium in the presence of donor DNA, said donor DNA comprising said DNA not naturally occurring in vaccinia virus present within a segment of vaccinia DNA, whereby said DNA not naturally occurring in vaccinia is introduced into the genome of said vaccinia virus, and then recovering vaccinia virus modified by such in vivo recombination.
Claim 6
Independent Claim
85 words
A method for the in vivo recombination of vaccinia virus DNA and DNA not naturally occurring in vaccinia virus, which method comprises infecting a cell with vaccinia virus in a cell-compatible medium in the presence of donor DNA, said donor DNA comprising said DNA not naturally occurring in vaccinia virus present within a segment of vaccinia DNA, whereby said DNA not naturally occurring in vaccinia is introduced into the genome of said vaccinia virus, and then recovering vaccinia virus modified by such in vivo recombination.
Claim 7
Dependent Claim
33 words
A method as in claim 4 wherein said segment of vaccinia DNA is co-linear with portions of the vaccinia genome except for the presence of said DNA not naturally occurring in vaccinia virus.
Claim 8
Dependent Claim
27 words
A method as in claim 5 wherein said DNA not naturally occurring in vaccinia virus is present within a non-essential region of said segment of vaccinia DNA.
Claim 9
Dependent Claim
15 words
A method as in claim 5 wherein said cell is present in a cell monolayer.
Claim 10
Independent Claim
100 words
A method for the in vivo recombination of vaccinia virus DNA with DNA not naturally occurring in vaccinia virus, which method comprises contacting a cell monolayer with a cell-compatible medium containing vaccinia virus and with a cell-compatible medium comprising donor DNA, said donor DNA comprising said DNA not naturally occurring in vaccinia virus present within a non-essential region of a segment of vaccinia virus otherwise co-linear with portions of the vaccinia genome, whereby said DNA not naturally occurring in vaccinia is introduced into the genome of said vaccinia virus, and then recovering vaccinia virus modified by such in vivo recombination.
Claim 11
Independent Claim
100 words
A method for the in vivo recombination of vaccinia virus DNA with DNA not naturally occurring in vaccinia virus, which method comprises contacting a cell monolayer with a cell-compatible medium containing vaccinia virus and with a cell-compatible medium comprising donor DNA, said donor DNA comprising said DNA not naturally occurring in vaccinia virus present within a non-essential region of a segment of vaccinia virus otherwise co-linear with portions of the vaccinia genome, whereby said DNA not naturally occurring in vaccinia is introduced into the genome of said vaccinia virus, and then recovering vaccinia virus modified by such in vivo recombination.
Claim 12
Dependent Claim
15 words
A method as in claim 8 wherein said donor DNA is clonable with a plasmid.
Claim 13
Dependent Claim
24 words
A method as in claim 8 wherein said DNA not naturally occurring in vaccinia virus comprises the Bam HI TK gene of herpes simplex.
Claim 14
Dependent Claim
19 words
A method as in claim 8 wherein said co-linear segment of vaccinia DNA is the Hind III F-fragment thereof.
Claim 15
Dependent Claim
21 words
A method as in claim 8 wherein said donor DNA is present as a calcium orthophosphate precipitate of said donor DNA.
Claim 16
Dependent Claim
19 words
A method as in claim 8 wherein said vaccinia virus is free of a vaccinia gene producing thymidine kinase.
Claim 17
Independent Claim
30 words
The method of replicating DNA in a eukaryotic cell by infecting said cell with a vaccinia virus modified to contain said DNA, said DNA not naturally occurring in vaccinia virus.
Claim 18
Independent Claim
30 words
The method of replicating DNA in a eukaryotic cell by infecting said cell with a vaccinia virus modified to contain said DNA, said DNA not naturally occurring in vaccinia virus.
Claim 19
Dependent Claim
17 words
A method as in claim 14 wherein said DNA is also not naturally occurring in said cell.
Claim 20
Dependent Claim
14 words
A method as in claim 14 wherein said DNA is expressed by said cell.
Claim 21
Dependent Claim
14 words
A method as in claim 15 wherein said DNA is expressed by said cell.
Claim 22
Dependent Claim
22 words
A method as in claim 16 wherein said DNA is expressed by the production by said cell of a biologically active protein.
Claim 23
Dependent Claim
22 words
A method as in claim 17 wherein said DNA is expressed by the production by said cell of a biolgically active protein.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-09-06 | 1992-03-07 | 1992-09-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-09-06 | 1996-03-07 | 1996-09-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-09-06 | 2000-03-07 | 2000-09-06 | $7700.00 | Window Closed / Lapsed |