US4753983A
Expired / Lapsed
US
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Polymeric matrix for affinity chromatography and immobilization of ligands
Assignee: UNISYN FIBERTEC Corp A CORP OF CALIFORNIA
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Filing: 1986-05-07
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Granted: 1988-06-28
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Expires: 2006-05-07
Abstract
Abstract A matrix useful for immobilizing organic ligands, such as biologically active materials, is prepared by reacting a hydroxyl group containing polymer with polyethyleneimine.
Inventors
- 👤 That T. Ngo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 20:55
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3202; B01J20/3206; B01J20/3208; B01J20/321; B01J20/22; B01J20/26; B01J20/261; B01J20/262; B01J20/265; B01J20/267; B01J20/28; B01J20/28014; B01J20/28047; B01J20/281; B01J20/286; B01J20/289; B01J20/291; B01J20/3212; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3255; B01J20/3268; B01J20/3272; B01J20/328; C; C08; C08F; C08F8/00; C08F8/30; B01J2220/00; B01J2220/40; B01J2220/48; B01J2220/4812; B01J2220/4825; B01J2220/4831; B01J2220/50; B01J2220/54; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
76 words
A process for preparing an affinity chromatographic matrix comprising the steps of: reacting a polymeric substance containing at least one hydroxyl group with 2-fluoro-1-methylpyridinium toluene-4-sulfonate to form an activated polymer wherein at least some of the hydroxyl groups of said polymer have been converted to 1-methyl-2-pyridoxy groups; and reacting said 1-methyl-2-pyridoxy substituted polymer with polyethyleneimine to form an affinity chromatographic matrix in which at least some of the 1-methyl-2-pyridoxy groups have been replaced by said polyethyleneimine.
Claim 2
Dependent Claim
76 words
A process for preparing an affinity chromatographic matrix comprising the steps of: reacting a polymeric substance containing at least one hydroxyl group with 2-fluoro-1-methylpyridinium toluene-4-sulfonate to form an activated polymer wherein at least some of the hydroxyl groups of said polymer have been converted to 1-methyl-2-pyridoxy groups; and reacting said 1-methyl-2-pyridoxy substituted polymer with polyethyleneimine to form an affinity chromatographic matrix in which at least some of the 1-methyl-2-pyridoxy groups have been replaced by said polyethyleneimine.
Claim 3
Dependent Claim
13 words
A process according to claim 1 wherein said polymeric substance is a polysaccharide.
Claim 4
Dependent Claim
12 words
A process according to claim 2 wherein said polysaccharide is a dextran.
Claim 5
Dependent Claim
13 words
A process according to claim 2 wherein said polysaccharide is a cross-linked dextran.
Claim 6
Dependent Claim
12 words
A process according to claim 2 wherein said polysaccharide is an agarose.
Claim 7
Dependent Claim
13 words
A process according to claim 2 wherein said polysaccharide is a cross-linked agarose.
Claim 8
Dependent Claim
11 words
A process according to claim 2 wherein said polysaccharide is cellulose.
Claim 9
Dependent Claim
17 words
A process according to claim 1 wherein said polymeric substance is in the form of a gel.
Claim 10
Dependent Claim
15 words
A process according to claim 1 wherein said polymeric substance is a polymer of N-acryloyl-2-amino-2-hydroxymethyl-1,3-propanediol.
Claim 11
Dependent Claim
14 words
A process according to claim 1 wherein said polymeric substance is a polyvinyl alcohol.
Claim 12
Dependent Claim
12 words
A process according to claim 1 wherein said polymeric substance is paper.
Claim 13
Dependent Claim
19 words
A process according to claim 1 wherein said polymeric substance is reacted with said 2-fluoro-1-methylpyridinium toluene-4-sulfonate in aqueous solution.
Claim 14
Dependent Claim
21 words
A process according to claim 1 wherein said polymeric substance is reacted with said 2-fluoro-1-methylpyridinium toluene-4-sulfonate in a polar organic solvent.
Claim 15
Dependent Claim
100 words
A process for preparing an affinity chromatographic matrix comprising the steps of: reacting a polymeric substance containing at least one hydroxyl group with 2-fluoro-1-methylpyridinium toluene-4-sulfonate to form an activated polymer wherein at least some of the hydroxyl groups of said polymer have been converted to 1-methyl-2-pyridoxy groups; reacting said 1-methyl-2-pyridoxy substituted polymer with polyethyleneimine to form a polyethyleneimine substituted polymer in which at least some of the 1-methyl-2-pyridoxy groups of said activated polymer have been replaced by said polyethyleneimine; and reacting said polyethyleneimine substituted polymer with an electrophilic ligand capable of reacting with an amino group of said polyethyleneimine-substituted polymer.
Claim 16
Dependent Claim
100 words
A process for preparing an affinity chromatographic matrix comprising the steps of: reacting a polymeric substance containing at least one hydroxyl group with 2-fluoro-1-methylpyridinium toluene-4-sulfonate to form an activated polymer wherein at least some of the hydroxyl groups of said polymer have been converted to 1-methyl-2-pyridoxy groups; reacting said 1-methyl-2-pyridoxy substituted polymer with polyethyleneimine to form a polyethyleneimine substituted polymer in which at least some of the 1-methyl-2-pyridoxy groups of said activated polymer have been replaced by said polyethyleneimine; and reacting said polyethyleneimine substituted polymer with an electrophilic ligand capable of reacting with an amino group of said polyethyleneimine-substituted polymer.
Claim 17
Dependent Claim
15 words
A process according to claim 14 wherein said electrophilic ligand is a aralkyl carboxylic acid.
Claim 18
Dependent Claim
13 words
A process according to claim 14 wherein said electrophilic ligand is ethylene sulfide.
Claim 19
Dependent Claim
30 words
A process according to claim 14 wherein said electrophilic ligand is ethylene sulfide and the product of reaction of said polyethyleneimine substituted polymer with ethylene sulfide is reacted with 2,2-dithiopyridine.
Claim 20
Dependent Claim
15 words
A process according to claim 14 wherein said electrophilic ligand is a carboxylic acid anhydride.
Claim 21
Dependent Claim
12 words
An affinity chromatographic matrix prepared according to the process of claim 1.
Claim 22
Dependent Claim
12 words
An affinity chromatographic matrix prepared according to the process of claim 14.
Claim 23
Dependent Claim
12 words
An affinity chromatographic matrix prepared according to the process of claim 15.
Claim 24
Dependent Claim
12 words
An affinity chromatographic matrix prepared according to the process of claim 16.
Claim 25
Dependent Claim
12 words
An affinity chromatographic matrix prepared according to the process of claim 17.
Claim 26
Dependent Claim
12 words
An affinity chromatographic matrix prepared according to the process of claim 18.
References Cited (Backward Citations)
US4195128A
Cited
US4415665A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-06-28 | 1991-12-28 | 1992-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-06-28 | 1995-12-28 | 1996-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-06-28 | 1999-12-28 | 2000-06-28 | $7700.00 | Window Closed / Lapsed |