US4748119A
Expired / Lapsed
US
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Process for altering and regulating gene expression
Assignee: Individual
•
Filing: 1987-01-15
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Granted: 1988-05-31
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Expires: 2007-01-15
Abstract
Abstract A process for altering the potential for Z-DNA formation which comprises altering the nucleotide sequence of DNA in vitro by site-directed mutagenesis or DNA substitution or deletion. Six rules for altering potential for Z-DNA formation are provided. These include the alternations of purine and pyrimidine residues.
Inventors
- 👤 Alexander Rich
- 👤 Alfred E. Nordheim
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 14:01
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/67
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
27 words
A process for enhancing the potential for Z-DNA formation in a nucleotide sequence which comprises altering said nucleotide sequence to have alternations of purine and pyrimidine residues.
Claim 2
Dependent Claim
27 words
A process for enhancing the potential for Z-DNA formation in a nucleotide sequence which comprises altering said nucleotide sequence to have alternations of purine and pyrimidine residues.
Claim 3
Dependent Claim
20 words
A process, according to claim 1, wherein said alternations of purine and pyrimidine residues are CG, CA, TG, or TA.
Claim 4
Dependent Claim
23 words
A process, according to claim 1, wherein said alternations of purine and pyrimidine residues are at least five base pairs in total length.
Claim 5
Dependent Claim
15 words
A process, according to claim 1, wherein said alternations are substantially purine and pyrimidine residues.
Claim 6
Dependent Claim
18 words
A process, according to claim 1, wherein said alternations of purine and pyrimidine residues are predominantly CG sequences.
Claim 7
Dependent Claim
35 words
A process, according to claim 1, wherein said alternations of purine and pyrimidine residues on adjoining DNA segments are out of transcriptional reading phase and said DNA segments are greater than five nucleotide bases each.
Claim 8
Dependent Claim
26 words
A process, according to claim 1, wherein said alternations of purine and pyrimidine residues exist in pairs separated by about 40 to about 80 nucleotide bases.
Claim 9
Dependent Claim
28 words
A process for diminishing the potential for Z-DNA formation in a nucleotide sequence which comprises deletion of a DNA segment which has alternations of purine and pyrimidine residues.
Claim 10
Dependent Claim
28 words
A process for diminishing the potential for Z-DNA formation in a nucleotide sequence which comprises deletion of a DNA segment which has alternations of purine and pyrimidine residues.
Claim 11
Dependent Claim
18 words
A process, according to claim 10, wherein said DNA segment is about 5 to about 15 base pairs.
Claim 12
Dependent Claim
23 words
A process, according to claim 8, which comprises deleting alternations of purine and pyrimidine residues to less than five base pairs in length.
Claim 13
Dependent Claim
22 words
A process, according to claim 8, which comprises inserting nucleotide base segments which are out of alternation with purine and pyrimidine residues.
Claim 14
Dependent Claim
18 words
A process, according to claim 8, which comprises deleting pyrimidine-purine alternations, particularly CG sequences in said nucleotide sequence.
Claim 15
Dependent Claim
26 words
A process, according to claim 8, which comprises deletion of about 40 to about 80 nucleotide bases separating pairs of alternations of purine and pyrimidine residues.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-31 | 1991-11-30 | 1992-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-31 | 1995-11-30 | 1996-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-31 | 1999-11-30 | 2000-05-31 | $7700.00 | Window Closed / Lapsed |