US4748111A
Expired / Lapsed
US
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Nucleic acid-protein conjugate used in immunoassay
Assignee: Molecular Diagnostics Inc
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Filing: 1984-03-12
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Granted: 1988-05-31
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Expires: 2004-03-12
Abstract
Abstract A protein is covalently coupled to a 3'terminal end of a nucleic acid which carries several labels. In an assay the protein will specifically recognize some component of a test system; in an immunoassay the protein can be Protein A which will recognize the FC portion of IgG which is bound to an unknown antigen if present in the test sample.
Inventors
- 👤 Nanibhushan Dattagupta
- 👤 William J. Knowles
- 👤 Vincent T. Marchesi
- 👤 Donald M. Crothers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 08:20
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/682; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; Y; Y10; Y10S; Y10S435/00; Y10S435/964; Y10S436/00; Y10S436/828
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
76 words
An assay for detecting an antigen comprising: combining a sample suspected of containing an antigen with an antibody specific to said antigen and a protein selected from the group consisting of Protein A and an antibody, said protein coupled to a 3' terminal end of a nucleic acid wherein the nucleic acid carries one or more label molecules; allowing complexation of said antigen, if contained in the sample, antibody and protein; incubating the resultant complex, and
Claim 2
Dependent Claim
76 words
An assay for detecting an antigen comprising: combining a sample suspected of containing an antigen with an antibody specific to said antigen and a protein selected from the group consisting of Protein A and an antibody, said protein coupled to a 3' terminal end of a nucleic acid wherein the nucleic acid carries one or more label molecules; allowing complexation of said antigen, if contained in the sample, antibody and protein; incubating the resultant complex, and
Claim 3
Dependent Claim
15 words
assaying for the label which is indicative of the amount of antigen in the sample.
Claim 4
Dependent Claim
15 words
assaying for the label which is indicative of the amount of antigen in the sample.
Claim 5
Dependent Claim
22 words
An assay according to calim 1, wherein the nucleic acid is single or double stranded RNA or DNA, or a RNA-DNA hybrid.
Claim 6
Dependent Claim
22 words
An assay according to calim 1, wherein the nucleic acid is single or double stranded RNA or DNA, or a RNA-DNA hybrid.
Claim 7
Dependent Claim
38 words
An assay according to claim 1, wherein the label carried by the nucleic acid is selected from the group consisting of a fluorescent moiety, and antigen, a hapten, an enzyme, a radioactive isotope, a cofactor and a carbohydrate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-31 | 1991-11-30 | 1992-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-31 | 1995-11-30 | 1996-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-31 | 1999-11-30 | 2000-05-31 | $7700.00 | Window Closed / Lapsed |