US4740470A
Expired / Lapsed
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Biologically functional molecular chimeras
Assignee: Leland Stanford Junior University
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Filing: 1984-04-20
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Granted: 1988-04-26
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Expires: 2004-04-20
Abstract
Abstract Method and compositions are provided for replication and expression of exogenous genes in microorganisms. Plasmids or virus DNA are cleaved to provide linear DNA having ligatable termini, which are bound to a gene having complementary termini, to provide a biologically functional replicon with a desired phenotypical property. The replicon is inserted into a microorganism cell by transformation. Isolation of the transformants provides cells for replication and expression of the DNA molecules present in the modified plasmid. The method provides a convenient and efficient way to introduce genetic capability into microorganisms for the production of nucleic acids and proteins, such as medically or commercially useful enzymes, which may have direct usefulness, or may find expression in the production of drugs, such as hormones, antibiotics, or the like, fixation of nitrogen, fermentation, utilization of specific feedstocks, or the like. The invention was supported by generous grants of NIH, NSF and the American Cancer Society.
Inventors
- 👤 Stanley N. Cohen
- 👤 Herbert W. Boyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:37
CPC Classifications
C; C12; C12N; C12N15/00; Y; Y10; Y10S; Y10S435/00; Y10S435/82
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
153 words
A method for replicating biologically functional circular recombinant DNA molecules in transformed host cells, which comprises: (1) preparing biologically functional circular recombinant DNA molecules by: joining first and second DNA linear segments, said first and second DNA segments having termini of a predetermined character at least in part complementary, at least one of said first and second DNA linear segments having a gene for a phenotypical trait, said first DNA linear segment having an intact extrachromosomal replicon recognized by said host cell, and said second DNA linear segment having foreign DNA derived from a source which does not exchange genetic information with said host cells; (2) transforming said host cells with said biologically functional recombinant DNA molecules; (3) growing said host cells under appropriate nutrient conditions; and (4) isolating said transformant cells with said biologically functional recombinant DNA molecules by means of said phenotypical trait imparted by said biologically functional recombinant DNA molecules.
Claim 2
Independent Claim
153 words
A method for replicating biologically functional circular recombinant DNA molecules in transformed host cells, which comprises: (1) preparing biologically functional circular recombinant DNA molecules by: joining first and second DNA linear segments, said first and second DNA segments having termini of a predetermined character at least in part complementary, at least one of said first and second DNA linear segments having a gene for a phenotypical trait, said first DNA linear segment having an intact extrachromosomal replicon recognized by said host cell, and said second DNA linear segment having foreign DNA derived from a source which does not exchange genetic information with said host cells; (2) transforming said host cells with said biologically functional recombinant DNA molecules; (3) growing said host cells under appropriate nutrient conditions; and (4) isolating said transformant cells with said biologically functional recombinant DNA molecules by means of said phenotypical trait imparted by said biologically functional recombinant DNA molecules.
Claim 3
Dependent Claim
13 words
A method according to claim 1 wherein said host cells are unicellular organisms.
Claim 4
Dependent Claim
13 words
A method according to claim 1, wherein said host cells are prokaryotic organisms.
Claim 5
Independent Claim
51 words
A method according to claims 1, 2 or 3 wherein said foreign DNA of said segment includes an intact gene capable of expression in said host cells, and including the additional step of growing said isolated transformant cells in an appropriate medium, whereby said intact gene is expressed and protein produced.
Claim 6
Independent Claim
158 words
A method for producing foreign RNA from biologically functional circular recombinant DNA molecules in transformed host cells, which comprises: (1) preparing biologically functional circular recombinant DNA molecules by: joining first and second DNA linear segments, said first and second DNA segments having termini of a predetermined character at least in part complementary, at least one of, said first and second linear DNA segments having a gene for a phenotypical trait, said first DNA linear segment having an intact extrachromsomal replicon recognized by said host cells, and said second DNA linear segment having foreign DNA derived from a source which does not exchange genetic information with said host cells; (2) transforming said host cells with said biologically functional recombinant DNA molecules; (3) growing said host cells under appropriate nutrient conditions; and (4) isolating said RNA containing transformant cells with said biologically functional recombinant DNA molecules by means of said phenotypical trait imparted by said biologically functional recombinant DNA molecules.
Claim 7
Independent Claim
158 words
A method for producing foreign RNA from biologically functional circular recombinant DNA molecules in transformed host cells, which comprises: (1) preparing biologically functional circular recombinant DNA molecules by: joining first and second DNA linear segments, said first and second DNA segments having termini of a predetermined character at least in part complementary, at least one of, said first and second linear DNA segments having a gene for a phenotypical trait, said first DNA linear segment having an intact extrachromsomal replicon recognized by said host cells, and said second DNA linear segment having foreign DNA derived from a source which does not exchange genetic information with said host cells; (2) transforming said host cells with said biologically functional recombinant DNA molecules; (3) growing said host cells under appropriate nutrient conditions; and (4) isolating said RNA containing transformant cells with said biologically functional recombinant DNA molecules by means of said phenotypical trait imparted by said biologically functional recombinant DNA molecules.
Claim 8
Independent Claim
157 words
A method for replicating biologically functional circular recombinant DNA molecules in transformed host cells, which comprises: (1) preparing biologically functional circular recombinant DNA molecules by: joining first and second DNa linear segments, said first and second DNA segments having termini of a predetermined character at least in part complementary, at least one of said first and second DNA linear segments having a gene for a phenotypical trait, said first DNA linear segment having an intact replicon recognized by said host cells from plasmid or viral DNA, and said second DNA linear segment having foreign DNA derived from a source which does not exchange genetic information with said host cells; (2) transforming said host cells with said biologically functional recombinant DNA molecules; (3) growing said host cells under appropriate nutrient conditions; and (4) isolating said transformant cells with said biologically functional recombinant DNA molecules by means of said phenotypical trait imparted by said biologically functional recombinant DNA molecules.
Claim 9
Independent Claim
157 words
A method for replicating biologically functional circular recombinant DNA molecules in transformed host cells, which comprises: (1) preparing biologically functional circular recombinant DNA molecules by: joining first and second DNa linear segments, said first and second DNA segments having termini of a predetermined character at least in part complementary, at least one of said first and second DNA linear segments having a gene for a phenotypical trait, said first DNA linear segment having an intact replicon recognized by said host cells from plasmid or viral DNA, and said second DNA linear segment having foreign DNA derived from a source which does not exchange genetic information with said host cells; (2) transforming said host cells with said biologically functional recombinant DNA molecules; (3) growing said host cells under appropriate nutrient conditions; and (4) isolating said transformant cells with said biologically functional recombinant DNA molecules by means of said phenotypical trait imparted by said biologically functional recombinant DNA molecules.
Claim 10
Dependent Claim
13 words
A method according to claim 6, wherein said host cells are unicellular organisms.
Claim 11
Dependent Claim
13 words
A method according to claim 6, wherein said host cells are prokaryotic organisms.
Claim 12
Dependent Claim
64 words
As an article of manufacture, a cloned biologically functional circular recombinant DNA molecule capable of selection and replication in a host cell comprising: a first DNA segment containing an intact extrachromsomal replicon recognized by said host cell, said first segment being joined to a second DNA segment having foreign DNA derived from a source which does not exchange genetic information with said host cell.
Claim 13
Dependent Claim
64 words
As an article of manufacture, a cloned biologically functional circular recombinant DNA molecule capable of selection and replication in a host cell comprising: a first DNA segment containing an intact extrachromsomal replicon recognized by said host cell, said first segment being joined to a second DNA segment having foreign DNA derived from a source which does not exchange genetic information with said host cell.
Claim 14
Dependent Claim
16 words
An article of manufacture according to claim 9, wherein said host cell is a unicellular organism.
Claim 15
Dependent Claim
16 words
An article of manufacture according to claim 9, wherein said host cell is a prokaryotic organism.
Claim 16
Dependent Claim
77 words
As an article of manufacture, a cloned biologically functional circular recombinant DNA molecule capable of selection and replication in a host cell comprising: a first DNA segment containing an intact replicon recognized by said host cell from plasmid or viral DNA joined to a second DNA segment comprising a foreign gene capable of expression in said host cell wherein said foreign gene is derived from a source which does not exchange genetic information with said host cell.
Claim 17
Dependent Claim
77 words
As an article of manufacture, a cloned biologically functional circular recombinant DNA molecule capable of selection and replication in a host cell comprising: a first DNA segment containing an intact replicon recognized by said host cell from plasmid or viral DNA joined to a second DNA segment comprising a foreign gene capable of expression in said host cell wherein said foreign gene is derived from a source which does not exchange genetic information with said host cell.
Claim 18
Dependent Claim
16 words
An article of manufacture according to claim 12, wherein said host cell is a unicellular organism.
Claim 19
Dependent Claim
16 words
An article of manufacture according to claim 12, wherein said host cell is a prokaryotic organism.
Claim 20
Dependent Claim
23 words
An article of manufacture according to claims 12, 13, or 14, wherein said gene has as a phenotypical property expression of an enzyme.
Claim 21
Dependent Claim
82 words
As an article of manufacture, a cloned biologically functional circular recombinant DNA molecule capable of selection and replication in a host cell comprising: cloned copies of a first DNA segment containing an intact extrachromsomal replicon recognized by said host cell from a plasmid or viral DNA joined to a second DNA segment comprising a foreign gene capable of expression in said host cell, wherein said foreign gene is derived from a source which does not exchange genetic information with said host cell.
Claim 22
Dependent Claim
82 words
As an article of manufacture, a cloned biologically functional circular recombinant DNA molecule capable of selection and replication in a host cell comprising: cloned copies of a first DNA segment containing an intact extrachromsomal replicon recognized by said host cell from a plasmid or viral DNA joined to a second DNA segment comprising a foreign gene capable of expression in said host cell, wherein said foreign gene is derived from a source which does not exchange genetic information with said host cell.
Claim 23
Dependent Claim
68 words
A transformant cell comprising a biologically functional circular recombinant DNA molecule capable of selection and replication in said cell, said DNA molecule comprising: a first DNA segment containing an intact extrachromsomal replicon recognized by said cell, said first segment being joined to a second DNA segment having foreign DNA, wherein said foreign DNA is derived from a source which does not exchange genetic information with said host cell.
Claim 24
Dependent Claim
68 words
A transformant cell comprising a biologically functional circular recombinant DNA molecule capable of selection and replication in said cell, said DNA molecule comprising: a first DNA segment containing an intact extrachromsomal replicon recognized by said cell, said first segment being joined to a second DNA segment having foreign DNA, wherein said foreign DNA is derived from a source which does not exchange genetic information with said host cell.
Claim 25
Dependent Claim
14 words
A transformant cell according to claim 17, wherein said cell is a unicellular organism.
Claim 26
Dependent Claim
14 words
A transformant cell according to claim 17, wherein said cell is a prokaryotic organism.
Claim 27
Dependent Claim
80 words
A transformant cell comprising a biologically functional circular recombinant DNA molecule capable of selection and replication in said cell, said DNA molecule comprising: a first DNA segment containing an intact extrachromsomal replicon recognized by said cell from plasmid or viral DNA, said first segment being joined to a second DNA segment comprising a foreign gene capable of expression in said cell wherein said foreign gene is derived from a source which does not exchange genetic information with said host cell.
Claim 28
Dependent Claim
80 words
A transformant cell comprising a biologically functional circular recombinant DNA molecule capable of selection and replication in said cell, said DNA molecule comprising: a first DNA segment containing an intact extrachromsomal replicon recognized by said cell from plasmid or viral DNA, said first segment being joined to a second DNA segment comprising a foreign gene capable of expression in said cell wherein said foreign gene is derived from a source which does not exchange genetic information with said host cell.
Claim 29
Dependent Claim
14 words
A transformant cell according to claim 20, wherein said cell is a unicellular organism.
Claim 30
Dependent Claim
14 words
A transformant cell according to claim 20, wherein said cell is a prokaryotic organism.
Claim 31
Dependent Claim
21 words
A transformant cell according to claims 20, 21, or 22 wherein said cell includes the expression product of said foreign gene.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-26 | 1991-10-26 | 1992-04-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-26 | 1995-10-26 | 1996-04-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-26 | 1999-10-26 | 2000-04-26 | $7700.00 | Window Closed / Lapsed |