US4737458A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Aminopyrazolinones, reagent containing them and the use thereof in the enzymatic determinaton of hydrogen peroxide
Assignee: Roche Diagnostics GmbH
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Filing: 1986-08-08
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Granted: 1988-04-12
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Expires: 2006-08-08
Abstract
Abstract The present invention provides aminopyrazolinones of the general formula: <IMAGE> wherein R1, R2 and R3, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 carbon atoms and together containing at most 8 carbon atoms. The present invention also provides a process for the preparation of these new aminopyrazolinones and reagents containing them. In addition, the present invention is concerned with the use of these new aminopyrazolinones as a color coupler for the detection of hydrogen peroxide.
Inventors
- 👤 Hans-Georg Batz
- 👤 Rupert Herrmann
- 👤 Joachim Siedel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:13
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C07; C07D; C07D231/00; C07D231/02; C07D231/10; C07D231/14; C07D231/44; C07D231/46; C12Q2326/00; C12Q2326/90; C12Q2326/96
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
51 words
An aminopyrazolinone of the formula: ##STR5## wherein R 1 is C 1 -C 6 alkyl, R 2 is C 1 -C 6 alkyl and R 3 is C 1 -C 6 alkyl, with the proviso that R 1 , R 2 and R 3 together total 3 to 8 carbon atoms.
Claim 2
Dependent Claim
51 words
An aminopyrazolinone of the formula: ##STR5## wherein R 1 is C 1 -C 6 alkyl, R 2 is C 1 -C 6 alkyl and R 3 is C 1 -C 6 alkyl, with the proviso that R 1 , R 2 and R 3 together total 3 to 8 carbon atoms.
Claim 3
Dependent Claim
1 words
4-Amino-1,2,3-trimethylpyrazolin-5-one.
Claim 4
Dependent Claim
1 words
4-Amino-1,2,3-trimethylpyrazolin-5-one.
Claim 5
Dependent Claim
13 words
The aminopyrazolinone of claim 1 wherein R 1 =R 2 =R 3 .
Claim 6
Dependent Claim
11 words
The aminopyrazolinone of claim 1 wherein R 2 =R 3 .
Claim 7
Dependent Claim
13 words
The aminopyrazolinone of claim 1 wherein R 2 =R 3 =methyl or ethyl.
Claim 8
Dependent Claim
12 words
The aminopyrazolinone of claim 1 designated 1,2,3-triethyl-pyrazolin-5-one, 1,2-dimethyl-3-isopropylpyrazolin-5-one, 1-n-butyl-2,3-diethylpyrazolin-5-one, 1-tert-butyl-2,3-dimethyl-pyrazolin-5-one or 1-hexyl-2,3-dimethylpyrazolin-5-one.
Claim 9
Dependent Claim
86 words
In a method of detection of hydrogen peroxide the improvement wherein, an aminopyrazolinone of the formula: ##STR6## wherein R 1 is C 1 -C 6 alkyl, R 2 is C 1 -C 6 alkyl and R 3 is C 1 -C 6 alkyl, with the proviso that R 1 , R 2 and R 3 together total 3 to 8 carbon atoms, is used as a colour coupler for the detection of hydrogen peroxide in the presence of peroxidase, with a phenolic or anilinic coupling component.
Claim 10
Dependent Claim
86 words
In a method of detection of hydrogen peroxide the improvement wherein, an aminopyrazolinone of the formula: ##STR6## wherein R 1 is C 1 -C 6 alkyl, R 2 is C 1 -C 6 alkyl and R 3 is C 1 -C 6 alkyl, with the proviso that R 1 , R 2 and R 3 together total 3 to 8 carbon atoms, is used as a colour coupler for the detection of hydrogen peroxide in the presence of peroxidase, with a phenolic or anilinic coupling component.
Claim 11
Dependent Claim
20 words
The method according to claim 7, wherein the coupling component is an anilinic coupling component selected from N-ethyl-N-hydroxyethyl-3-methylaniline and N-ethyl-N-(3-methylphenyl)-N'-acetamidoethylenediamine.
Claim 12
Dependent Claim
26 words
A reagent for the determination of hydrogen peroxide, containing peroxidase, at least one compound according to claim 1, at least one coupling component and a buffer.
References Cited (Backward Citations)
US3617167A
Cited
US4394512A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-12 | 1991-10-12 | 1992-04-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-12 | 1995-10-12 | 1996-04-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-12 | 1999-10-12 | 2000-04-12 | $7700.00 | Window Closed / Lapsed |