US4737258A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Element for electrophoresis containing polyacrylamide gel membrane
Assignee: Fujifilm Holdings Corp
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Filing: 1985-05-14
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Granted: 1988-04-12
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Expires: 2005-05-14
Abstract
Abstract An element for electrophoresis suitably employable for electrophoresis of biopolymers such as proteins, as well as for determination of base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic support, an adhesive layer containing a cellulose derivative, and an electrophoresis medium layer comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order. The electrophoresis medium layer may contain a water-soluble polymer and agarose. The medium layer may contain a modifier such as an anionic surfactant, formamide or urea.
Inventors
- 👤 Masashi Ogawa
- 👤 Masafumi Fukugawa
- 👤 Teppei Ikeda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 07:32
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; Y; Y10; Y10T; Y10T428/00; Y10T428/31504; Y10T428/31725; Y10T428/31779
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
72 words
An element for electrophoresis comprising: (I) a plastic material support; (II) an adhesive layer containing not less than 80 wt. percent of a cellulose derivative selected from the group consisting of diacetyl cellulose, triacetyl cellulose and nitrocellulose; and (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order.
Claim 2
Dependent Claim
72 words
An element for electrophoresis comprising: (I) a plastic material support; (II) an adhesive layer containing not less than 80 wt. percent of a cellulose derivative selected from the group consisting of diacetyl cellulose, triacetyl cellulose and nitrocellulose; and (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order.
Claim 3
Dependent Claim
20 words
The element for electrophoresis as claimed in claim 1 in which said adhesive layer consists essentially of said cellulose derivative.
Claim 4
Dependent Claim
22 words
The element for electrophoresis as claimed in claim 1 or 2 in which the plastic material sheet is a polyethylene terephthalate sheet.
Claim 5
Dependent Claim
22 words
The element for electrophoresis as claimed in claim 1 or 2 in which said medium layer contains a water-soluble polymer and agarose.
Claim 6
Dependent Claim
20 words
The element for electrophoresis as claimed in claim 1 or 2 in which said medium layer contains an anionic surfactant.
Claim 7
Dependent Claim
17 words
The element for electrophoresis as claimed in claim 5 in which said anionic surfactant is an alkylsulfate.
Claim 8
Dependent Claim
16 words
The element for electrophoresis as claimed in claim 6 in which said alkylsulfate is sodium dodecylsulfate.
Claim 9
Dependent Claim
25 words
The element for electrophoresis as claimed in claim 1 or 2 in which said medium layer contains a compound having at least one carbamoyl group.
Claim 10
Dependent Claim
23 words
The element for electrophoresis as claimed in claim 8 in which said compound having at least one carbamoyl group is urea or formamide.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-12 | 1991-10-12 | 1992-04-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-12 | 1995-10-12 | 1996-04-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-12 | 1999-10-12 | 2000-04-12 | $7700.00 | Window Closed / Lapsed |