US4732847A
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Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1982-12-29
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Granted: 1988-03-22
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Expires: 2002-12-29
Abstract
Abstract Monoclonal antibodies are provided capable of distinguishing DNA-RNA hybrid complexes from single stranded DNA and RNA and double stranded DNA and RNA. The antibodies find particular use in determining the presence of a specific nucleic acid sequence on a solid surface. Single stranded polynucleotide is fixed to a solid (gel) surface and then hybridized with the complementary probe. The hybrid complex specific monoclonal antibody is then added to bind to any hybrid complexes which have formed. By appropriate label, the hybrid complex may be visualized in a variety of ways.
Inventors
- 👤 W. Dorsey Stuart
- 👤 Mark B. Frank
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 19:29
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6804; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5308; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S530/00; Y10S530/808; Y10S530/809; Y10S530/863; Y10S530/864
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
23 words
IgM or IgG mouse monoclonal antibodies which specifically bind to DNA-RNA hybrid complexes but which do not bind to isolated RNA or DNA.
Claim 2
Dependent Claim
23 words
IgM or IgG mouse monoclonal antibodies which specifically bind to DNA-RNA hybrid complexes but which do not bind to isolated RNA or DNA.
Claim 3
Dependent Claim
8 words
Mouse IgG monoclonal antibodies according to claim 1.
Claim 4
Dependent Claim
9 words
Mouse IgM monoclonal antibodies according to claim 1. 1.
Claim 5
Dependent Claim
19 words
Monoclonal antibodies according to claim 1 labeled with a member of the group consisting of radionuclides, fluorescers or enzymes.
Claim 6
Independent Claim
125 words
A method for detecting the presence of a nucleotide sequence which comprises: fixing a DNA or RNA sequence to a solid support as a single strand; hybridizing said fixed single stranded nucleic acid sequence with a sequence of the other type of nucleic acid having a predetermined nucleic acid sequence to bind to any homologous fixed sequence to form a hybrid duplex; adding to said hybrid duplex mouse IgG or IgM monoclonal antibodies which specifically bind to DNA-RNA hybrid complexes, whereby said monoclonal antibodies bind to hybrid duplexes bound to said solid support; and determining the presence of monoclonal antibody bound to said solid support by means of a label providing a detectable signal, which label is bound directly or indirectly to said monoclonal antibody.
Claim 7
Independent Claim
125 words
A method for detecting the presence of a nucleotide sequence which comprises: fixing a DNA or RNA sequence to a solid support as a single strand; hybridizing said fixed single stranded nucleic acid sequence with a sequence of the other type of nucleic acid having a predetermined nucleic acid sequence to bind to any homologous fixed sequence to form a hybrid duplex; adding to said hybrid duplex mouse IgG or IgM monoclonal antibodies which specifically bind to DNA-RNA hybrid complexes, whereby said monoclonal antibodies bind to hybrid duplexes bound to said solid support; and determining the presence of monoclonal antibody bound to said solid support by means of a label providing a detectable signal, which label is bound directly or indirectly to said monoclonal antibody.
Claim 8
Dependent Claim
13 words
A method according to claim 5, wherein said fixed nucleotide sequence is DNA.
Claim 9
Dependent Claim
13 words
A method according to claim 6, wherein said fixed nucleotide sequence is chromosomal.
Claim 10
Dependent Claim
13 words
A method according to claim 5, wherein said fixed nucleotide sequence is RNA.
Claim 11
Dependent Claim
12 words
A method according to claim 8, wherein said RNA is messenger RNA.
Claim 12
Independent Claim
33 words
A method according to any one of claims 5, 6 or 8, wherein said label is bound to an antibody capable of specifically binding to said monoclonal antibody bound to said hybrid complex.
Claim 13
Dependent Claim
12 words
A method according to claim 10, wherein said label is a radionuclide.
Claim 14
Dependent Claim
12 words
A method according to claim 10, wherein said label is a fluorescer.
Claim 15
Dependent Claim
12 words
A method according to claim 10, wherein said label is an enzyme.
Claim 16
Dependent Claim
62 words
A kit for determining the presence in situ of a hybrid DNA-RNA complex which comprises primary IgM or IgG mouse monoclonal antibodies having the property of specifically binding to said complex but not to isolated DNA or RNA and secondary antibodies having the property specifically binding to said primary antibodies, said secondary antibodies having a label capable of providing a detectable signal.
Claim 17
Dependent Claim
62 words
A kit for determining the presence in situ of a hybrid DNA-RNA complex which comprises primary IgM or IgG mouse monoclonal antibodies having the property of specifically binding to said complex but not to isolated DNA or RNA and secondary antibodies having the property specifically binding to said primary antibodies, said secondary antibodies having a label capable of providing a detectable signal.
Claim 18
Dependent Claim
20 words
A kit according to claim 14, wherein said label is selected from the group consisting of radionuclides, fluorescers or enzymes.
References Cited (Backward Citations)
EP0144914A2
Cited
EP0146039A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-03-22 | 1991-09-21 | 1992-03-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-03-22 | 1995-09-21 | 1996-03-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-03-22 | 1999-09-21 | 2000-03-22 | $7700.00 | Window Closed / Lapsed |