Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization

Claim 2

A detection probe comprising a hybridizable single stranded portion of nucleic acid connected to a non-hybridizable, single stranded nucleic acid portion, the non-hybridizable portion including a recognition site for a particular protein, wherein the non-hybridizable portion has been modified to create the protein recognition site, wherein the modification is effected by connecting to said non-hybridizable portion a moiety selected from the group consisting of furocoumarin, phenanthridinium and a platinum-containing ligand.

Claim 3

A detection probe according to claim 1, wherein the hybridizable portion is covalently associated with one of the strands of the non-hybridizable portion.

Claim 4

A detection probe according to claim 1, wherein the hybridizable portion is complementary to the genomic sequence responsible for sickle cell anemia.

Claim 5

A detection probe according to claim 1, wherein the modifier is a furocoumarin carrying a protein binding site.

Claim 6

A detection probe according to claim 1, including a protein bound to the protein-specific site of the non-hybridizable portion.

Claim 7

A detection probe according to claim 5, wherein the protein carries a label.

Claim 8

A detection probe according to claim 5, wherein the protein is an antibody specific for a modified double stranded portion.

Claim 9

A detection probe according to claim 5, including a labeled protein bound to an immunoglobulin.

Claim 10

A detection probe according to claim 5, wherein the nucleic acid is antigenic and is bound through an immunoglobulin to a labeled protein.

Claim 11

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridizing conditions in the presence of a detection probe according to claim 1, binding to the non-hybridizable portion of the probe a protein specific thereto, labeling the protein at any stage in the process, and assaying the label.

Claim 12

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridization in the presence of a detection probe according to claim 1, and assaying the label.

Claim 13

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridization in the presence of a detection probe according to claim 1, reacting the modified non-hybridizable portion with an antibody specific therefor, and assaying the antibody.

Claim 14

A detection probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable single stranded nucleic acid portion, the non-hybridizable portion carrying a fluorophore.

Claim 15

A detection probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable single stranded nucleic acid portion, the non-hybridizable portion carrying a fluorophore.

Claim 16

A detection probe according to claim 13, wherein the fluorophore is coupled to the nucleic acid of the non-hybridizable portion by a furocoumarin.

Claim 17

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridization in the presence of a detection probe according to claim 14, the furocoumarin carrying a hapten or ligand, and assaying the hapten or ligand.

Claim 18

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridization in the presence of a detection probe according to claim 14, the furocoumarin carrying a fluorophore, and assaying the fluorophore.

Claim 19

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridization in the presence of a detection probe according to claim 13, and assaying the fluorophore.

Claim 20

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridization in the presence of a detection probe according to claim 1, binding to the non-hybridizable portion of the probe a protein specific thereto, labeling the protein at any stage in the process, separating the support from the balance of the material, dissociating the bound protein from the hybridized detection probe, and assaying the dissociated protein.

Claim 21

A test kit for assaying for the presence of a particular nucleic acid sequence comprising in one or more containers a detection probe according to claim 1, and a protein recognizable by the protein recognition site of the non-hybridizable portion.

Claim 22

A test kit for assaying for the presence of a particular nucleic acid sequence in a sample comprising in one or more containers a detection probe according to claim 1, a separation probe comprising a single stranded nucleic acid immobilized on a support, the single stranded nucleic acid and the hybridizable portion of the detection probe being hybridizable with the sample, and a protein recognizable by the protein recognition site of the non-hybridizable portion of the detection probe.

Claim 23

A detection probe comprising a hybridizable single stranded portion of nucleic acid connected to a non-hybridizable, double stranded nucleic acid portion, the non-hybridizable portion including a recognition site for a particular protein, wherein the non-hybridizable portion has been modified to create the protein recognition site, wherein the modification is effected by connecting to said non-hybridizable portion a moiety selected from the group consisting of furocoumarin, phenanthridinium and a platinum-containing ligand.

Claim 24

A detection probe comprising a hybridizable single stranded portion of nucleic acid connected to a non-hybridizable, double stranded nucleic acid portion, the non-hybridizable portion including a recognition site for a particular protein, wherein the non-hybridizable portion has been modified to create the protein recognition site, wherein the modification is effected by connecting to said non-hybridizable portion a moiety selected from the group consisting of furocoumarin, phenanthridinium and a platinum-containing ligand.

Claim 25

A detection probe according to claim 21, wherein the hybridizable portion is covalently associated with one of the strands of the non-hybridizable portion.

Claim 26

A detection probe according to claim 21, wherein the hybridizable portion is complementary to the genomic sequence responsible for sickle cell anemia.

Claim 27

A detection probe according to claim 21, wherein the modifier is a furocoumarin carrying a protein binding site.

Claim 28

A detection probe according to claim 21, including a protein bound to the protein-specific site of the non-hybridizable portion.

Claim 29

A detection probe according to claim 23, wherein the protein carries a label.

Claim 30

A detection probe according to claim 23, wherein the protein is an antibody specific for a modified double stranded portion.

Claim 31

A detection probe according to claim 23, wherein the protein is an immunoglobulin.

Claim 32

A detection probe according to claim 28, including a labeled protein bound to the immunoglobulin.

Claim 33

A detection probe according to claim 23, wherein the nucleic acid is antigenic and is bound through an immunoglobulin to a labeled protein.

Claim 34

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridizing conditions in the presence of a detection probe according to claim 21, binding to the non-hybridizable portion of the probe a protein specific thereto, labeling the protein at any stage in the process, and assaying the label.

Claim 35

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridization in the presence of a detection probe according to claim 21, and assaying the label.

Claim 36

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridization in the presence of a detection probe according to claim 21, reacting the modified non-hybridizable portion with an antibody specific therefor, and assaying the antibody.

Claim 37

A detectionn probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable double stranded nucleic acid portion, the non-hybridizable portion carrying a fluorophore.

Claim 38

A detectionn probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable double stranded nucleic acid portion, the non-hybridizable portion carrying a fluorophore.

Claim 39

A detection probe according to claim 34, wherein the fluorophore is coupled to the nucleic acid of the non-hybridizable portion by a furocoumarin.

Claim 40

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridizing conditions in the presence of a detection probe according to claim 24, the furocoumarin carrying a hapten or ligand, and assaying the hapten or ligand.

Claim 41

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridizing conditions in the presence of a detection probe according to claim 34, the furocoumarin carrying a fluorophore, and assaying the fluorophore.

Claim 42

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridizing conditions in the presence of a detection probe according to claim 34, and assaying the fluorophore.

Claim 43

A process for conducting an assay for the presence of a particular nucleic acid sequence comprising immobilizing either (a) a separation probe or (b) an unknown sample to be assayed on a solid support, subjecting the support to hybridizing conditions in the presence of a detection probe according to claim 21, binding to the non-hybridizable portion of the probe a protein specific thereto, labeling the protein at any stage in the process, separating the support from the balance of the material, dissociating the bound protein from the hybridized detection probe, and assaying the dissociated protein.

Claim 44

A test kit for assaying for the presence of a particular nucleic acid sequence comprising in one or more containers a detection probe according to claim 21, and a protein recognizable by the protein recognition site of the non-hybridizable portion.

Claim 45

A test kit for assaying for the presence of a particular nucleic acid sequence in a sample comprising in one or more containers a detection probe according to claim 21, a separation probe comprising a single stranded nucleic acid immobilized on a support, the single stranded nucleic acid and the hybridizable portion of the detection probe being hybridizable with the sample, and a protein recognizable by the protein recognition site of the non-hybridizable portion of the detection probe.

Claim 46

A method for determining whether the DNA contained in a test sample includes a particular nucleic acid sequence, comprising the steps of: (a) extracting nucleic acids from the test sample, (b) digesting the extracted nucleic acids with a restriction enzyme thereby to cleave the DNA or not at a particular sequence, depending on whether or not a restriction enzyme recognition site is present in the sequence, (c) treating the product of step (b) to form single-stranded nucleic acids, (d) contacting the single-stranded nucleic acids produced in step (c) with a first and a second polynucleotide probe, each of said probes being complementary to respective first and second portions of said sequence to be detected, said first probe being in solution with said test sample, said first probe being a probe according to claim 1, said second probe being attached to a solid support, the two portions being non-overlapping and immediately adjacent to the restriction site in question, the restriction site being between the first and second portions, such contact being performed under conditions favorable to hybridization of said first and second probes to said sequence to be detected, hybridizationn with both of said probes being dependent upon whether in step (b) restriction did not occur, said first probe being incorporated with distinguishable label, (e) separating, by means of said second probe, (i) any resulting dual hybridization product comprising said sequence to be detected hybridized to both said labeled first probe and said second probe, from (ii) any unhybridized and singly hybridized labeled first probe, and (f) detecting by means of said label any of said separated dual hybridization product which may be present.

Claim 47

A method for determining whether the DNA contained in a test sample includes a particular nucleic acid sequence, comprising the steps of: (a) extracting nucleic acids from the test sample, (b) digesting the extracted nucleic acids with a restriction enzyme thereby to cleave the DNA or not at a particular sequence, depending on whether or not a restriction enzyme recognition site is present in the sequence, (c) treating the product of step (b) to form single-stranded nucleic acids, (d) contacting the single stranded nucleic acids produced in step (c) with a first and a second polynucleotide probe, each of said probes being complementary to respective first and second portions of said sequence to be detected, said first probe being in solution with said test sample, said first probe being a probe according to claim 27, said second probe being attached to a solid support, the two portions being non-overlapping and immediately adjacent to the restriction site in question, the restriction site being between the first and second portions, such contact being performed under conditions favorable to hybridization of said first and second probes to said sequence to be detected, hybridization with both of said probes being dependent upon whether in step (b) restriction did not occur, said first probe being incorporated with a distinguishable label, (e) separating, by means of said second probe, (i) any resulting dual hybridization product comprising said sequence to be detected hybridized to both said labeled first probe and said second probe, from (ii) any unhybridized and singly hybridized labeled first probe, and (f) detecting by means of said label any of said separated dual hybridization product which may be present.

Claim 48

A detection probe according to claim 4, wherein the protein binding site comprises biotin.

Claim 49

A detection probe according to claim 24, wherein the protein binding site comprises biotin.

Claim 50

A detection probe comprising a hybridizable single stranded portion of nucleic acid connected to a non-hybridizable, single straded nucleic acid portion, the non-hybridizable portion modified by a fluorescent moiety.

Claim 51

A detection probe comprising a hybridizable single stranded portion of nucleic acid connected to a non-hybridizable, single straded nucleic acid portion, the non-hybridizable portion modified by a fluorescent moiety.

Claim 52

A detection probe according to claim 46, wherein the fluorescent moiety is linked to a furocourmarin.

Claim 53

A detection probe according to claim 46, wherein the fluorescent moiety comprises fluorescein.

Claim 54

A detection probe comprising a hybridizable double stranded portion of nucleic acid connected to a non-hybridizable, double stranded nucleic acid portion, the non-hybridizable portion modified by a fluoroescent moiety.

Claim 55

A detection probe comprising a hybridizable double stranded portion of nucleic acid connected to a non-hybridizable, double stranded nucleic acid portion, the non-hybridizable portion modified by a fluoroescent moiety.

Claim 56

A detection probe according to claim 49, wherein the fluorescent moiety is linked to a furocourmarin.

Claim 57

A detection probe according to claim 49, wherein the fluorescent moiety comprises fluorescein.