US4721669A
Expired / Lapsed
US
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Chemical probes for left-handed DNA and chiral metal complexes as Z-specific anti-tumor agents
Assignee: Columbia University
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Filing: 1985-01-18
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Granted: 1988-01-26
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Expires: 2005-01-18
Abstract
Abstract This invention concerns a coordination complex and salts and optically resolved enantiomers thereof, of the formula (R)3-M, wherein R comprises 1,10-phenanthroline or a substituted derivative thereof, M comprises a suitable transition metal, e.g. ruthenium(II) or cobalt(III), and R is bonded by M by a coordination bond. The complexes of this invention are useful in methods for labeling, nicking and cleaving DNA. The lambda enantiomer of complexes of this invention is useful in methods for specifically labeling, detecting, nicking and cleaving Z-DNA. The complexes may also be used in a method for killing tumor cells and may be combined with a pharmaceutically acceptable carrier to form a pharmaceutical composition for the treatment of tumor cells in a subject. The invention further concerns methods for treating a subject afflicted with tumor cells.
Inventors
- 👤 Jacqueline K. Barton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:52
CPC Classifications
C; C07; C07D; C07D471/00; C07D471/02; C07D471/04; C07F; C07F15/00; C07F15/06; C07F15/065; C07H; C07H21/00; C07H23/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
91 words
A method for detecting in a sample the presence of double-stranded DNA comprising contacting the sample with a complex of the formula (R) 3 --Co (III), wherein R comprises 1,10-phenanthroline or a substituted derivative thereof and R is bound to Co(III) by a coordination bond, the contacting being effected under conditions such that the complex binds to double-stranded DNA present in the sample by intercalation and thereby labels the double-stranded DNA with the complex, detecting DNA labeled with the complex, and thereby detecting the presence of double-stranded DNA in the sample.
Claim 2
Independent Claim
91 words
A method for detecting in a sample the presence of double-stranded DNA comprising contacting the sample with a complex of the formula (R) 3 --Co (III), wherein R comprises 1,10-phenanthroline or a substituted derivative thereof and R is bound to Co(III) by a coordination bond, the contacting being effected under conditions such that the complex binds to double-stranded DNA present in the sample by intercalation and thereby labels the double-stranded DNA with the complex, detecting DNA labeled with the complex, and thereby detecting the presence of double-stranded DNA in the sample.
Claim 3
Dependent Claim
25 words
A method of claim 1, wherein the substituted derivative of 1,10-phenanthroline comprises 4,7-diamino-1,10-phenanthroline; 3,8-diamino-1,10-phenanthroline; 4,7-diethylenediamine-1,10-phenanthroline; 3,8-diethylenediamine-1,10-phenanthroline; 4,7-dihydroxyl-1,10-phenanthroline; 3,8-dihydroxyl-1,10-phenanthroline; 4,7-dinitro-1,10-phenanthroline; 3,8-dinitro-1,10-phenanthroline; 4,7-diphenyl-1,10-phenanthroline; 3,8-diphenyl-1,10-phenanthroline; 4,7-dispermine-1,10-phenanthroline, or 3,8-dispermine-1,10-phenanthroline.
Claim 4
Independent Claim
110 words
A method for detecting in a sample the presence of double-stranded Z-DNA which comprises contacting the sample with the lambda enantiomer of a complex having the formula (R) 3 --M, wherein R comprises 1,10-phenanthroline or a substituted derivative thereof, M comprises ruthenium (II) or cobalt (III) and R is bound to M by a coordination bond, the contacting being effected under conditions such that the complex binds to double-stranded Z-DNA present in the sample by intercalation and thereby labels the double-stranded Z-DNA with the lambda enantiomer of the complex, detecting Z-DNA labeled with the lambda enantiomer of the complex, and thereby detecting the presence of double-stranded Z-DNA in the sample.
Claim 5
Independent Claim
110 words
A method for detecting in a sample the presence of double-stranded Z-DNA which comprises contacting the sample with the lambda enantiomer of a complex having the formula (R) 3 --M, wherein R comprises 1,10-phenanthroline or a substituted derivative thereof, M comprises ruthenium (II) or cobalt (III) and R is bound to M by a coordination bond, the contacting being effected under conditions such that the complex binds to double-stranded Z-DNA present in the sample by intercalation and thereby labels the double-stranded Z-DNA with the lambda enantiomer of the complex, detecting Z-DNA labeled with the lambda enantiomer of the complex, and thereby detecting the presence of double-stranded Z-DNA in the sample.
Claim 6
Dependent Claim
9 words
A method of claim 3 wherein M is ruthenium(II).
Claim 7
Dependent Claim
9 words
A method of claim 3, wherein M is cobalt(III).
Claim 8
Dependent Claim
26 words
A method of claim 3, wherein the substituted derivative of 1,10 -phenanthroline comprises 4,7-diamino-1,10-phenanthroline; 3,8-diamino-1,10-phenanthroline; 4,7-diethylenediamine-1,10-phenanthroline; 3,8-diethylenediamine-1,10-phenanthroline; 4,7-dihydroxyl-1,10-phenanthroline; 3,8-dihydroxyl-1,10-phenanthroline; 4,7-dinitro-1,10-phenanthroline; 3,8-dinitro-1,10-phenanthroline; 4,7-diphenyl-1,10-phenanthroline; 3,8-diphenyl-1,10-phenanthroline; 4,7-dispermine-1,10-phenanthroline, or 3,8-dispermine-1,10-phenanthroline.
Claim 9
Independent Claim
105 words
A method for nicking double-stranded DNA by effecting breakage of at least one phosphodiester bond along the DNA which comprises contacting the DNA with a coordination complex or salt thereof having the formula (R) 3 --Co (III), wherein R comprises 1,10-phenanthroline or a substituted derivative thereof and R is bound to Co by a coordination bond, under conditions such that the complex binds to the DNA by intercalation to form an adduct and irradiating the adduct so formed with ultraviolet radiation of a wavelength which is absorbed by the ligand bands of the complex so as to nick the DNA at the site(s) of intercalation.
Claim 10
Independent Claim
105 words
A method for nicking double-stranded DNA by effecting breakage of at least one phosphodiester bond along the DNA which comprises contacting the DNA with a coordination complex or salt thereof having the formula (R) 3 --Co (III), wherein R comprises 1,10-phenanthroline or a substituted derivative thereof and R is bound to Co by a coordination bond, under conditions such that the complex binds to the DNA by intercalation to form an adduct and irradiating the adduct so formed with ultraviolet radiation of a wavelength which is absorbed by the ligand bands of the complex so as to nick the DNA at the site(s) of intercalation.
Claim 11
Dependent Claim
58 words
A method for cleaving double-stranded DNA which comprises nicking the DNA according to claim 7 and treating the nicked DNA so produced with an enzyme which is not deactivated in the presence of the complex used for DNA nicking and capable of cleaving single-stranded DNA so as to cleave the nicked DNA at the site of the nick(s).
Claim 12
Independent Claim
123 words
A method for selectively nicking Z-DNA, present in DNA comprising Z-DNA and other forms of DNA, by effecting breakage of at least one phosphodiester bond along the Z-DNA which comprises contacting the DNA containing the Z-DNA with a Λ isomer of a coordination complex or salt thereof having the formula (R) 3 --CO(III), wherein R comprises 1,10-phenanthroline or a substituted derivative thereof and R is bound to Co by a coordination bond, under conditions such that the Λ isomer binds to the DNA by intercalation to form an adduct and irradiating the adduct so formed with ultraviolet radiation of a wavelength which is absorbed by the ligand bands of the Λ isomer so as to nick the DNA at the site(s) of intercalation.
Claim 13
Independent Claim
123 words
A method for selectively nicking Z-DNA, present in DNA comprising Z-DNA and other forms of DNA, by effecting breakage of at least one phosphodiester bond along the Z-DNA which comprises contacting the DNA containing the Z-DNA with a Λ isomer of a coordination complex or salt thereof having the formula (R) 3 --CO(III), wherein R comprises 1,10-phenanthroline or a substituted derivative thereof and R is bound to Co by a coordination bond, under conditions such that the Λ isomer binds to the DNA by intercalation to form an adduct and irradiating the adduct so formed with ultraviolet radiation of a wavelength which is absorbed by the ligand bands of the Λ isomer so as to nick the DNA at the site(s) of intercalation.
Claim 14
Dependent Claim
69 words
A method for selectively cleaving double-stranded Z-DNA, present in DNA comprising Z-DNA and other forms of DNA, which comprises nicking the Z-DNA according to claim 9 and treating the nicked DNA so produced with an enzyme which is not deactivated in the presence of the complex used for DNA nicking and capable of cleaving single-stranded DNA so as to cleave the nicked DNA at the site of the nick(s).
References Cited (Backward Citations)
US4395486A
Cited
US4547569A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-01-25 | 1991-07-27 | 1992-01-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-01-25 | 1995-07-27 | 1996-01-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-01-25 | 1999-07-27 | 2000-01-26 | $7700.00 | Window Closed / Lapsed |