US4713340A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biodegradation of pentachlorophenol
Assignee: University of Minnesota Twin Cities
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Filing: 1984-06-13
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Granted: 1987-12-15
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Expires: 2004-06-13
Abstract
Abstract A bacterium of the genus Flavobacterium which utilizes pentachlorophenol (PCP) as its sole carbon and energy source, which tolerates media PCP concentrations over about 250 mg/l, and which may be used in methods of detoxifying PCP-contaminated material.
Inventors
- 👤 Ronald L. Crawford
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 16:03
CPC Classifications
A; A62; A62D; A62D3/00; A62D3/02; B; B09; B09B; B09B3/00; B09B3/60; B09C; B09C1/00; B09C1/10; C; C02; C02F; C02F3/00; C02F3/02; C02F3/12; C02F3/1205; C02F3/1231; C02F3/34; C12; C12N; C12N1/00; C12N1/20; C12N1/205; A62D2101/00; A62D2101/20; A62D2101/22; C12R; C12R2001/00; C12R2001/01; C12R2001/20; Y; Y02; Y02W; Y02W10/00; Y02W10/10; Y10; Y10S; Y10S210/00; Y10S210/902; Y10S210/908; Y10S210/909; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/85
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
56 words
A method of detoxifying pentachlorophenol (PCP) by microbial degradation comprising culturing a PCP-degrading bacterium in an aqueous medium comprising dissolved PCP, wherein said bacterium is a member of the genus Flavobacterium, and is capable of sustained growth in medium concentrations of PCP between 250 mg/l and 400 mg/l as its sole source of carbon and energy.
Claim 2
Independent Claim
56 words
A method of detoxifying pentachlorophenol (PCP) by microbial degradation comprising culturing a PCP-degrading bacterium in an aqueous medium comprising dissolved PCP, wherein said bacterium is a member of the genus Flavobacterium, and is capable of sustained growth in medium concentrations of PCP between 250 mg/l and 400 mg/l as its sole source of carbon and energy.
Claim 3
Dependent Claim
50 words
The method of claim 1 wherein said method comprises inducing a PCP-degradation pathway of said bacterium by first culturing it in a first medium that is free of PCP and comprises a carbon and energy source other than PCP, and then adding an amount of PCP to said first medium.
Claim 4
Dependent Claim
15 words
The method of claim 2 wherein said source of carbon and energy is glutamic acid.
Claim 5
Dependent Claim
13 words
The method of claim 1 wherein said bacterium exhibits an insoluble yellow pigment.
Claim 6
Dependent Claim
19 words
The method of claim 1 wherein said bacterium is cultured at a temperature between 15° C. and 37° C.
Claim 7
Dependent Claim
20 words
The method of claim 1 wherein said bacterium has the characteristics of ATCC deposit No. 39723 and the mutations thereof.
Claim 8
Dependent Claim
36 words
A biologically pure culture of a strain of the genus Flavobacterium, wherein said strain exhibits sustained growth in media comprising pentachlorophenol (PCP) as the sole carbon and energy source at media PCP concentrations of 250-400 mg/l.
Claim 9
Dependent Claim
36 words
A biologically pure culture of a strain of the genus Flavobacterium, wherein said strain exhibits sustained growth in media comprising pentachlorophenol (PCP) as the sole carbon and energy source at media PCP concentrations of 250-400 mg/l.
Claim 10
Dependent Claim
13 words
The culture of claim 7 wherein said strain exhibits an insoluble yellow pigment.
Claim 11
Dependent Claim
21 words
The culture of claim 7 wherein said strain is grown in said PCP-containing media at a temperature of about 20°-37° C.
Claim 12
Dependent Claim
17 words
The culture of claim 7, having the identifying characteristics of ATCC NO. 39723 and the mutations thereof.
References Cited (Backward Citations)
US3310477A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-12-14 | 1991-06-15 | 1991-12-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-12-14 | 1995-06-15 | 1995-12-15 | $3760.00 | Window Closed / Lapsed |
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