US4711844A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Modified signal peptides
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1983-03-09
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Granted: 1987-12-08
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Expires: 2003-03-09
Abstract
Abstract The invention discloses a method for producing modified signal peptides sequences derived from wild-type signal peptide sequences of the type that are capable of forming membrane-bound lipoproteins. Modified signal peptide sequences produced by the method of the invention are useful for increasing the secretion of heterologous gene products produced by transformed host organisms. The invention further discloses a method for producing recombinant DNA sequences in vivo.
Inventors
- 👤 Shing Chang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 20:05
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C07K14/52; C07K14/54; C07K14/55; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/11; C12N15/62; C12N15/625; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/034; C07K2319/036; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/30
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
47 words
A plasmid comprising a DNA sequence encoding a signal sequence comprising the sequence met lys leu trp phe ser thr leu lys leu lys lys ala ala ala val leu leu phe ser cys val ala leu ala gly ser ala asn asn gln thr asn ala.
Claim 2
Dependent Claim
47 words
A plasmid comprising a DNA sequence encoding a signal sequence comprising the sequence met lys leu trp phe ser thr leu lys leu lys lys ala ala ala val leu leu phe ser cys val ala leu ala gly ser ala asn asn gln thr asn ala.
Claim 3
Dependent Claim
15 words
The plasmid of claim 1 wherein the signal sequence from that of B. licheniformis penicillinase.
Claim 4
Dependent Claim
26 words
A plasmid comprising a DNA sequence encoding a signal sequence derived from B. licheniformis penicillinase wherein the cys 27 residue is altered to ser 27 .
Claim 5
Dependent Claim
26 words
A plasmid comprising a DNA sequence encoding a signal sequence derived from B. licheniformis penicillinase wherein the cys 27 residue is altered to ser 27 .
Claim 6
Dependent Claim
9 words
A microorganism transformed by the plasmid of claim 1.
Claim 7
Dependent Claim
9 words
A microorganism transformed by the plasmid of claim 2.
Claim 8
Dependent Claim
9 words
A microorganism transformed by the plasmid of claim 3.
References Cited (Backward Citations)
US4338397A
Cited
EP0121352A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-12-07 | 1991-06-08 | 1991-12-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-12-07 | 1995-06-08 | 1995-12-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-12-07 | 1999-06-08 | 1999-12-08 | $7700.00 | Window Closed / Lapsed |