US4710464A
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Transcription terminators
Assignee: Eli Lilly and Co
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Filing: 1984-09-27
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Granted: 1987-12-01
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Expires: 2004-09-27
Abstract
Abstract The present transcription terminators are DNA sequences characterized by their ability to terminate the transcription of DNA sequences. The present sequences are portable, have a variety of restriction endonuclease sites and are useful in controlling the expression in hosts of DNA sequences coding for prokaryotic, eukaryotic and viral proteins and polypeptides.
Inventors
- 👤 Ramamoorthy Belagaje
- 👤 Stuart A. Kuhstoss
- 👤 R. Nagaraja Rao
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 03:38
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/67; C12N15/74; C12N15/76
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
24 words
A DNA sequence selected from the group consisting of: ##STR6## wherein A is deoxyodenyl G is deoxyguanyl, C is deoxycytosyl and T is thymidyl.
Claim 2
Dependent Claim
24 words
A DNA sequence selected from the group consisting of: ##STR6## wherein A is deoxyodenyl G is deoxyguanyl, C is deoxycytosyl and T is thymidyl.
Claim 3
Dependent Claim
45 words
A recombinant DNA molecule comprising a recombinant DNA and a DNA sequence selected from the group consisting of ##STR7## wherein A is deoxyodenyl G is deoxyguanyl, C is deoxycytosyl and T is thymidyl, said DNA sequence being located in said recombinant DNA in either orientation.
Claim 4
Dependent Claim
45 words
A recombinant DNA molecule comprising a recombinant DNA and a DNA sequence selected from the group consisting of ##STR7## wherein A is deoxyodenyl G is deoxyguanyl, C is deoxycytosyl and T is thymidyl, said DNA sequence being located in said recombinant DNA in either orientation.
Claim 5
Dependent Claim
22 words
The recombinant DNA molecule of claim 2 wherein said molecule is selected form the group consisting of pKC413, pKC414, pKC421 and pKC422.
Claim 6
Dependent Claim
35 words
The recombinant DNA molecule of claim 2 wherein at least one of said DNA sequences is located downstream of the stop codon of a DNA sequence in said molecule that codes for a desired polypeptide.
Claim 7
Dependent Claim
39 words
The recombinant DNA molecule of claim 2 wherein at least one of said DNA sequences is located upstream of a promoter sequence in such molecule that controls the expression of a DNA sequence that codes for a desired polypeptide.
Claim 8
Dependent Claim
39 words
The recombinant DNA molecule of claim 4 wherein at least one of said DNA sequences is located in said molecule upstream of a promoter sequence that controls the expression of the DNA sequence that codes for the desired polypeptide.
Claim 9
Dependent Claim
58 words
The recombinant DNA molecule of claim 5 wherein said promoter is selected from the group consisting of the trp promoter, the lac promoter, the TAC promoter, the lpp promoter, a promoter of bacteriophage phage λ, the β-lactamase promoter, the Bacillus veg promoter, the Staphylococcus nuclease promoter, a promoter that controls the expession of prokaryotic, eukaryotic and viral genes.
Claim 10
Dependent Claim
58 words
The recombinant DNA molecule of claim 6 wherein said promoter is selected from the group consisting of the trp promoter, the lac promoter, the TAC promoter, the Ipp promoter, a promoter of bacteriophage phage λ, the β-lactamase promoter, the Bacillus veg promoter, the Staphylococcus nuclease promoter, a promoter that controls the expression of prokaryotic, eukaryotic and viral genes.
Claim 11
Dependent Claim
12 words
A microbial host transformed with a recombinant DNA molecule of claim 2.
Claim 12
Dependent Claim
12 words
A microbial host transformed with a recombinant DNA molecule of claim 3.
Claim 13
Dependent Claim
12 words
A microbial host transformed with a recombinant DNA molecule of claim 4.
Claim 14
Dependent Claim
12 words
A microbial host transormed with a recombinant DNA molecule of claim 5.
Claim 15
Dependent Claim
12 words
A microbial host transformed with a recombinant DNA molecule of claim 6.
Claim 16
Dependent Claim
23 words
The microbial host according to claim 9, wherein the host is selected from the group consisting of E. coli, Bacillus, Streptomyces, and yeast.
Claim 17
Dependent Claim
22 words
The microbial host of claim 10 wherein the host is selected from the group consisting of E. coli, Bacillus, Streptomyces, and yeast.
Claim 18
Dependent Claim
21 words
The microbial host of claim 15 wherein the host selected from the group consisting of E. coli, Bacillus, Streptomyces, and yeast.
Claim 19
Dependent Claim
22 words
The microbial host of claim 12 wherein the host is selected from the group consisting of E. coli, Bacillus, Streptomyces, and yeast.
Claim 20
Dependent Claim
23 words
The microbial host of claim 13 wherein the untransformed host is selected from the group consisting of E. coli, Bacillus, Streptomyces, and yeast.
Claim 21
Dependent Claim
31 words
A method of terminating transcription of a DNA sequence comprising the step of inserting a DNA sequence of claim 1 downstream of the DNA sequence whose transcription is to be terminated.
Claim 22
Dependent Claim
31 words
A method of terminating transcription of a DNA sequence comprising the step of inserting a DNA sequence of claim 1 upstream of the DNA sequence whose transcription is to be terminated.
Claim 23
Dependent Claim
38 words
A method for reducing tanscription of a DNA sequence in a Streptomyces host cell that comprises placing a transcription terminator of claim 1 between the promoter driving transcription and the DNA sequence whose transcription is to be reduced.
Claim 24
Dependent Claim
17 words
The method of claim 21, wherein said transcription terminator is placed in a recombinant DNA expression vector.
References Cited (Backward Citations)
US4513086A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-11-30 | 1991-06-01 | 1991-12-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-30 | 1995-06-01 | 1995-12-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-30 | 1999-06-01 | 1999-12-01 | $7700.00 | Window Closed / Lapsed |