US4704356A
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Method of diagnosing cartilage tissue abnormalities
Assignee: RUSH-PESBYTERIAN-ST LUKE'S
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Filing: 1984-03-27
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Granted: 1987-11-03
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Expires: 2004-03-27
Abstract
Abstract It is found that abnormal levels of keratan sulfate in the peripheral blood are indicative of abnormalities of cartilage or cartilage-like tissues. Specifically, elevated levels of keratan sulfate in the peripheral blood plasma or serum are indicative of osteoarthritis and either substantially complete absence of or very elevated levels of keratan sulfate in the peripheral blood are indicative of muscular dystrophy and related disorders. The level of keratan sulfate in the peripheral blood is determined by an immunoassay using a monoclonal antibody. Preferably the immunoassay employs a colorimetric reporter system.
Inventors
- 👤 Eugene J. Thonar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 15:13
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6887; G01N2800/00; G01N2800/10; G01N2800/105; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/815
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
62 words
A method for indicating an abnormality of cartilage, disc or corneal tissue in a mammal, which method comprises measuring, by an immunoassay of a specimen of the peripheral blood serum or plasma of the mammal employing a monoclonal antibody reactive with keratan sulfate, the level of keratan sulfate in said specimen and noting an abnormal level of keratan sulfate in said specimen.
Claim 2
Independent Claim
62 words
A method for indicating an abnormality of cartilage, disc or corneal tissue in a mammal, which method comprises measuring, by an immunoassay of a specimen of the peripheral blood serum or plasma of the mammal employing a monoclonal antibody reactive with keratan sulfate, the level of keratan sulfate in said specimen and noting an abnormal level of keratan sulfate in said specimen.
Claim 3
Dependent Claim
11 words
A method according to claim 1 wherein the mammal is human.
Claim 4
Dependent Claim
30 words
A method according to claim 2 wherein the abnormality to be indicated is osteoarthritis and the level of keratan sulfate in the specimen of serum or plasma is abnormally high.
Claim 5
Dependent Claim
32 words
A method according to claim 2 wherein the abnormality to be indicated is mascular corneal dystrophy and the level of keratan sulfate in the specimen of serum or plasma is abnormally low.
Claim 6
Dependent Claim
14 words
A method according to claim 3 wherein the immunoassay is an enzyme-linked immunosorbent assay.
Claim 7
Dependent Claim
14 words
A method according to claim 4 wherein the immunoassay is an enzyme-linked immunosorbent assay.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-02 | 1991-05-04 | 1991-11-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-02 | 1995-05-04 | 1995-11-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-02 | 1999-05-04 | 1999-11-03 | $7700.00 | Window Closed / Lapsed |