Abstract The present invention is based on the discovery that amyotrophic lateral sclerosis (ALS), Parkinson disease and Alzheimer disease are due to lack of a disorder-specific neurotrophic hormone. Diagnosis is accomplished by assaying hormones specific for a particular neuronal network or system: the motor neurotrophic hormones from muscle in the motor neural system are used to diagnose and treat ALS, dopamine neurotrophic hormones from striatum in the nigrostriatal neural system are used to diagnose and treat parkinsonism, and cholinergic neurotrophic hormones released from the cortex and hippocampus which are specific for cholinergic neurons of the nucleus basalis and septal nucleus are used to diagnose and treat Alzheimer's disease. With tissue culture, the presence or absence of specific neurotrophic hormones can be assessed in ALS, parkinsonism, and Alzheimer disease. If there is a deficiency, extracted and purified neurotrophic hormones specific to the particular neuronal network or system can be injected in ALS and Alzheimer disease and in parkinsonism.
A method to diagnose the presence of Alzheimer's Disease in a subject by detecting a deficiency of a specific neurotrophic activity in said subject as compared to normal controls, wherein said deficiency is detected by the method which comprises: extracting the proteins from the hippocampal tissue of said subject; assaying said extract for neurotrophic activity with respect to the neuronal system normally associated with said hippocampal tissue; and comparing said neurotrophic activity to activity exhibited in the same assay by similar extracts from hippocampel tissue of controls.
Claim 2
Independent Claim
87 words
A method to diagnose the presence of Alzheimer's Disease in a subject by detecting a deficiency of a specific neurotrophic activity in said subject as compared to normal controls, wherein said deficiency is detected by the method which comprises: extracting the proteins from the hippocampal tissue of said subject; assaying said extract for neurotrophic activity with respect to the neuronal system normally associated with said hippocampal tissue; and comparing said neurotrophic activity to activity exhibited in the same assay by similar extracts from hippocampel tissue of controls.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein the extract is prepared by sonicating said hippocampal tissue in buffer and recovering the extract.
Claim 4
Dependent Claim
18 words
The method of claim 2 wherein said hippocampal extract is further purified to isolate a protein of 2kd-5kd.
Claim 5
Dependent Claim
19 words
The method of claim 1 wherein said neurotrophic activity assay comprises treating medial septal nucleus tissue with said extract.