US4699887A
Expired / Lapsed
US
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Electrochemical determination of hematocrit
Assignee: Dade Behring Inc
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Filing: 1986-03-24
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Granted: 1987-10-13
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Expires: 2006-03-24
Abstract
Abstract A method for measuring hematocrit is provided. An electrochemically detectable marker is measured before and after the ratio of red cell volume to plasma volume is decreased. The measured concentrations are used in a mathematical formula to determine hematocrit value.
Inventors
- 👤 Scot D. Abbott
- 👤 Herman W. Levin
- 👤 Robert K. Kobos
- 👤 Henn Kilkson
- 👤 Dale R. Peterson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/50; G01N33/86; Y; Y10; Y10T; Y10T436/00; Y10T436/13
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
116 words
A method for measuring the hematocrit of blood, Hct, comprising: (1) measuring concentration of a preselected electrochemically detectable marker, (Marker)WB, naturally present in a preselected volume of whole blood having an initial ratio of red cell volume to plasma volume, the concentration of marker naturally present within red cells being different from its concentration in plasma; (2) decreasing the ratio of red cell volume to plasma volume by lysing red cells in the preselected volume of whole blood; (3) measuring the marker concentration after lysing, (Marker)WB; and (4) calculating the hematocrit using an equation comprising the term: ##EQU4## where: Hct is hematocrit: (Marker)WB is measured marker concentration after Lysis; (Marker)WB is measured marker concentration before Lysis.
Claim 2
Independent Claim
116 words
A method for measuring the hematocrit of blood, Hct, comprising: (1) measuring concentration of a preselected electrochemically detectable marker, (Marker)WB, naturally present in a preselected volume of whole blood having an initial ratio of red cell volume to plasma volume, the concentration of marker naturally present within red cells being different from its concentration in plasma; (2) decreasing the ratio of red cell volume to plasma volume by lysing red cells in the preselected volume of whole blood; (3) measuring the marker concentration after lysing, (Marker)WB; and (4) calculating the hematocrit using an equation comprising the term: ##EQU4## where: Hct is hematocrit: (Marker)WB is measured marker concentration after Lysis; (Marker)WB is measured marker concentration before Lysis.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein the electrochemically detectable marker is an ion.
Claim 4
Dependent Claim
26 words
The method of claim 2 wherein the ion is selected from the group consisting of sodium ion, potassium ion, trimethylphenyl ammonium ion and tetrapentyl ammonium ion.
Claim 5
Dependent Claim
11 words
The method of claim 3 wherein the ion is sodium ion.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-10-12 | 1991-04-13 | 1991-10-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-10-12 | 1995-04-13 | 1995-10-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-10-12 | 1999-04-13 | 1999-10-13 | $7700.00 | Window Closed / Lapsed |