US4699828A
Expired / Lapsed
US
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Fluorescently labeled microbeads
Assignee: Becton Dickinson and Co
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Filing: 1986-06-30
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Granted: 1987-10-13
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Expires: 2006-06-30
Abstract
Abstract Highly uniform polymeric microbeads of a predetermined size, preferably having an attached fluorescent label on the surface, are prepared by causing seed particles to swell by absorption of a plurality of low molecular weight substances, at least one of which is a monomer having a reactive functional group. After absorption, the monomer is internally polymerized, and the reactive functional group is preferably reacted with a polyfunctional spacing agent thereby to provide a second reactive functional group. The latter is then reacted with a fluorescent label to provide the labeled microbead.
Inventors
- 👤 Abraham Schwartz
- 👤 Joel Williams
- 👤 Robert D. Stevens
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 23:00
CPC Classifications
C; C08; C08F; C08F8/00; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
155 words
Microbeads comprising polymeric seed particles, a swelling agent having a molecular weight less than about 300 and a solubility in water less than about 10 -2 grams per liter and from about 0.1% to about 50% by weight of a polymer of the formula: ##STR6## wherein R and R 1 are independently selected from the group consisting of hydrogen and lower alkyl of 1 to 6 carbon atoms, A is selected from the group consisting of a methylene or carbonyl group, X is selected from the group consisting of NH, oxygen or sulfur, Q is selected from the group consisting of a straight or branched chain alkylene group of 2 to 10 carbon atoms, an aryl group or a heterocyclic group, Y is selected from the group consisting of amino, hydroxyl, sulfhydryl or carbonyl, said microbeads having a diameter from about 1 to 50 microns and having a size distribution within 10% of the mean.
Claim 2
Dependent Claim
155 words
Microbeads comprising polymeric seed particles, a swelling agent having a molecular weight less than about 300 and a solubility in water less than about 10 -2 grams per liter and from about 0.1% to about 50% by weight of a polymer of the formula: ##STR6## wherein R and R 1 are independently selected from the group consisting of hydrogen and lower alkyl of 1 to 6 carbon atoms, A is selected from the group consisting of a methylene or carbonyl group, X is selected from the group consisting of NH, oxygen or sulfur, Q is selected from the group consisting of a straight or branched chain alkylene group of 2 to 10 carbon atoms, an aryl group or a heterocyclic group, Y is selected from the group consisting of amino, hydroxyl, sulfhydryl or carbonyl, said microbeads having a diameter from about 1 to 50 microns and having a size distribution within 10% of the mean.
Claim 3
Dependent Claim
153 words
Microbeads comprising polymeric seed particles, a swelling agent selected from the group consisting of a hydrocarbon and a halogenated hydrocarbon, said swelling agent having about 10-16 carbon atoms, and from about 0.1% to about 50% b weight of a polymer of the formula: ##STR7## wherein R and R 1 are independently selected from the group consisting of hydrogen and lower alkyl of 1 to 6 carbon atoms, A is selected from the group consisting of a methylene or carbonyl group, X is selected from the group consisting of NH, oxygen or sulfur, Q is selected from the group consisting of a straight or branched chain alkylene group 2 to 10 carbon atoms, an aryl group or a heterocyclic group, Y is selected from the group consisting of amino, hydroxyl, sulfhydryl or carbonyl said microbeads having a diameter from about 1 to 50 microns and having a size distribution within 10% of the mean.
Claim 4
Dependent Claim
153 words
Microbeads comprising polymeric seed particles, a swelling agent selected from the group consisting of a hydrocarbon and a halogenated hydrocarbon, said swelling agent having about 10-16 carbon atoms, and from about 0.1% to about 50% b weight of a polymer of the formula: ##STR7## wherein R and R 1 are independently selected from the group consisting of hydrogen and lower alkyl of 1 to 6 carbon atoms, A is selected from the group consisting of a methylene or carbonyl group, X is selected from the group consisting of NH, oxygen or sulfur, Q is selected from the group consisting of a straight or branched chain alkylene group 2 to 10 carbon atoms, an aryl group or a heterocyclic group, Y is selected from the group consisting of amino, hydroxyl, sulfhydryl or carbonyl said microbeads having a diameter from about 1 to 50 microns and having a size distribution within 10% of the mean.
Claim 5
Dependent Claim
13 words
The microbeads of claim 2 wherein said halogenated hydrocarbon is a chlorinated hydrocarbon.
References Cited (Backward Citations)
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