US4699705A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Element for electrophoresis using aqueous polyacrylamide gel
Assignee: DIRECTOR OF FINANCE DIVISION MINISTER'S SECRETARIAT SCIENCE AND TECHNOLOGY AGENCY
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Filing: 1986-04-21
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Granted: 1987-10-13
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Expires: 2006-04-21
Abstract
Abstract An element for electrophoresis comprising a polymer sheet having been subjected to glow discharge treatment and an aqueous gel medium layer containing a compound having at least one carbamoyl group (modifier, e.g., urea and formamide) and being provided on the sheet. The aqueous gel medium layer preferably comprises an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water. Further, the medium layer preferably contains a water-soluble polymer and agarose.
Inventors
- 👤 Masashi Ogawa
- 👤 Hiroyuki Tamaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 22:39
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; C; C12; C12Q; C12Q1/00; C12Q1/68
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
81 words
An element for electrophoresis comprising: a polymer sheet having been subjected to glow discharge treatment; and an aqueous polyacrylamide gel medium layer which is provided on the polymer sheet and contains a water-soluble polymer selected from the group consisting of polyacrylamide and polyethylene glycol, agarose and a compound selected from the group consisting of urea and formamide, the aqueous polyacrylamide gel medium layer being formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water.
Claim 2
Dependent Claim
81 words
An element for electrophoresis comprising: a polymer sheet having been subjected to glow discharge treatment; and an aqueous polyacrylamide gel medium layer which is provided on the polymer sheet and contains a water-soluble polymer selected from the group consisting of polyacrylamide and polyethylene glycol, agarose and a compound selected from the group consisting of urea and formamide, the aqueous polyacrylamide gel medium layer being formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water.
Claim 3
Dependent Claim
19 words
The element for electrophoresis as claimed in claim 1, in which said polymer sheet is a polyethylene terephthalate sheet.
Claim 4
Dependent Claim
39 words
The element for electrophoresis as claimed in claim 1 in which a polymer sheet having been subjected to glow discharge treatment is provided on said aqueous gel medium layer thereby sandwiching the medium layer between the two polymer sheets.
Claim 5
Dependent Claim
51 words
The element for electrophoresis as claimed in claim 1 in which said water-soluble polymer is contained in the amount of 2 to 100 wt. % based on the polyacrylamide solid amount and said agarose is contained in the aqueous polyacrylamide gel medium in the amount of 0.2 to 2 wt/v %.
Claim 6
Dependent Claim
19 words
The element for electrophoresis as claimed in claim 4 in which said polymer sheet is a polyethylene terephthalate sheet.
Claim 7
Dependent Claim
39 words
The element for electrophoresis as claimed in claim 4 in which a polymer sheet having been subjected to glow discharge treatment is provided on said aqueous gel medium layer thereby sandwiching the medium layer between the two polymer sheets.
Claim 8
Dependent Claim
46 words
The element for electrophoresis as claimed in claim 1 in which the water soluble polymer and agarose are dispersed in a three-dimensionally crosslinked polymer formed by the polymerization reaction of the acrylamide compound and the crosslinking agent and are entangled with the three-dimensionally crosslinked polymer structure.
Claim 9
Dependent Claim
127 words
In a method for determination of base sequence of a sample selected from the group consisting of DNA, RNA, their fragments and their derivatives in which the sample is electrophoresed in an acrylamide gel medium layer provided on a support sheet, the improvement which comprises using as the polyacrylamide gel medium layer for electrophoresis, and aqueous polyacrylamide gel medium layer which is provided on a polymer sheet having been subjected to glow discharge treatment and which contains a water-soluble polymer selected from the group consisting of polyacrylamide and polyethylene glycol, agarose, and a compound selected from the group consisting of urea and formamide, the aqueous polyacrylamide gel medium layer being formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water.
Claim 10
Dependent Claim
127 words
In a method for determination of base sequence of a sample selected from the group consisting of DNA, RNA, their fragments and their derivatives in which the sample is electrophoresed in an acrylamide gel medium layer provided on a support sheet, the improvement which comprises using as the polyacrylamide gel medium layer for electrophoresis, and aqueous polyacrylamide gel medium layer which is provided on a polymer sheet having been subjected to glow discharge treatment and which contains a water-soluble polymer selected from the group consisting of polyacrylamide and polyethylene glycol, agarose, and a compound selected from the group consisting of urea and formamide, the aqueous polyacrylamide gel medium layer being formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water.
Claim 11
Dependent Claim
49 words
The method as claimed in claim 8 in which said water-soluble polymer is contained in an amount of 2 to 100 wt. % based on the polyacrylamide solid amount and said agarose is contained in the aqueous polyacrylamide gel medium in an amount of 0.2 to 2 wt/v %.
Claim 12
Dependent Claim
43 words
The method as claimed in claim 8 in which said water-soluble polymer and agarose are dispersed in a three-dimensionally crosslinked polymer formed by the polymerization reaction of the acrylamide compound and the crosslinking agent and are entangled with the three-dimensionally crosslinked polymer structure.
References Cited (Backward Citations)
US4415428A
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US4481094A
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US4548870A
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US4548869A
Cited
US4579783A
Cited
US4600641A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-10-12 | 1991-04-13 | 1991-10-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-10-12 | 1995-04-13 | 1995-10-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-10-12 | 1999-04-13 | 1999-10-13 | $7700.00 | Window Closed / Lapsed |