US4697004A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing low phytate soy protein isolate
Assignee: Bristol Myers Co
•
Filing: 1985-09-06
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Granted: 1987-09-29
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Expires: 2005-09-06
Abstract
Abstract A high quality soy protein isolate with significantly reduced aluminum content and substantially free of phytic acid and phytate complexes is prepared by aqueous extraction of defatted particulated soybeans at pH 8 to 10, and at a temperature above 65 DEG C., separating the extract and then precipitating the protein out of solution, at a pH slightly higher than its isoelectric point, i.e., pH 5.3.
Inventors
- 👤 Gabor Puski
- 👤 Grant H. Hartman, Jr.
- 👤 Robert D. Talbott
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 12:29
CPC Classifications
A; A23; A23L; A23L11/00; A23J; A23J1/00; A23J1/14
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
139 words
A process for preparing a soy protein isolate with significantly reduced aluminum content and substantially free of phytate which comprises treating a defatted soybean raw material containing soy protein by forming a slurry with an aqueous medium at a pH within the range of pH 8 to 10 and at a temperature above 65° C. for a period of time sufficient to solubilize the soy protein; but reduce extraction of phytates; separating said slurry into an insoluble fraction containing a substantial portion of the polysaccharides and insoluble phytates and a soluble fraction which includes the solubilized soy protein; adjusting the pH of said soluble fraction above the isoelectric range to 5.0 to 5.5 to precipitate the soy protein and thereby produce a whey fraction containing soluble phytate and a soy protein solids fraction having a phytate concentration below 0.3%.
Claim 2
Dependent Claim
139 words
A process for preparing a soy protein isolate with significantly reduced aluminum content and substantially free of phytate which comprises treating a defatted soybean raw material containing soy protein by forming a slurry with an aqueous medium at a pH within the range of pH 8 to 10 and at a temperature above 65° C. for a period of time sufficient to solubilize the soy protein; but reduce extraction of phytates; separating said slurry into an insoluble fraction containing a substantial portion of the polysaccharides and insoluble phytates and a soluble fraction which includes the solubilized soy protein; adjusting the pH of said soluble fraction above the isoelectric range to 5.0 to 5.5 to precipitate the soy protein and thereby produce a whey fraction containing soluble phytate and a soy protein solids fraction having a phytate concentration below 0.3%.
Claim 3
Dependent Claim
12 words
The process of claim 1 wherein said soy protein fraction is dried.
Claim 4
Dependent Claim
29 words
The process of claim 1 wherein said soy protein fraction is formed into an aqueous solution at a pH in excess of the isoelectric range of said soy protein.
Claim 5
Dependent Claim
12 words
The process of claim 3 wherein said soy protein fraction is dried.
Claim 6
Dependent Claim
37 words
The process of claim 1 wherein said aqueous medium is water and said soy protein containing raw material is slurried therewith in a ratio of one part of raw material to 10-20 parts by weight of water.
Claim 7
Dependent Claim
26 words
The process of claim 5 wherein one part by weight of said soy protein containing raw material is slurried with 16 parts by weight of water.
Claim 8
Dependent Claim
47 words
The process of claim 1 wherein said soy protein raw material is maintained in said slurry at 70° C. to 85° C. for 1 to 15 minutes and for an additional 10 to 60 minutes after said slurry temperature is adjusted to 50° C. to 60° C.
Claim 9
Dependent Claim
36 words
The process of claim 7 wherein the pH of said soluble fraction is adjusted to pH 5.2 to 5.4 after the separation from said spent solids at a temperature of about 50° C. to 60° C.
Claim 10
Dependent Claim
19 words
The process of claim 8 wherein the pH of said solubilized soy protein solids is adjusted to pH 5.3.
Claim 11
Dependent Claim
15 words
The process of claim 1 wherein said defatted soybean raw material is defatted soybean flakes.
Claim 12
Dependent Claim
114 words
The process of claim 10 wherein one part by weight of said raw material is slurried with 16 parts by weight of water adjusted to pH 9 and at a temperature of 75° C. for 2 minutes, said slurry then cooled to 60° C. and stirred for an additional 10 minutes, said slurry then being separated into an insoluble fraction and a soluble fraction, discarding said insoluble fraction and adjusting the pH of said soluble fraction to 5.3 thereby precipitating a protein curd from said slurry, separating said protein curd, washing said protein curd with 10 parts by weight of water at pH 5.3, and separating said washed protein curd from said wash water.
Claim 13
Dependent Claim
12 words
The process of claim 11 wherein said washed protein curd is dried.
Claim 14
Dependent Claim
32 words
The process of claim 11 wherein the pH of said washed protein curd is formed into an aqueous solution at a pH in excess of the isoelectric range of said soy protein.
Claim 15
Dependent Claim
9 words
The product produced by the process of claim 1.
Claim 16
Dependent Claim
9 words
The product produced by the process of claim 2.
Claim 17
Dependent Claim
9 words
The product produced by the process of claim 4.
Claim 18
Dependent Claim
9 words
The product produced by the process of claim 6.
Claim 19
Dependent Claim
9 words
The product produced by the process of claim 9.
Claim 20
Dependent Claim
9 words
The product produced by the process of claim 11.
Claim 21
Dependent Claim
135 words
A process for preparing a soy protein isolate substantially free of phytate and with significantly reduced aluminum content which comprises in sequence the steps of: (a) forming an aqueous slurry of a defatted soybean raw material at a pH within the range of 8-10 at a temperature above 65° C. for a period of 1-15 minutes to solubilize the soy protein; (b) reducing the temperature to 25°-65° C. for a period of 10-60 minutes; (c) separating said slurry into an insoluble fraction and a soluble fraction which includes the solubilized soy protein; (d) adjusting the pH of said soluble fraction to pH 5.0 to 5.5 with a non-toxic water soluble acid at a temperature of 25°-65° C. thereby precipitating and separating said soy protein isolate substantially free of phytate and with significantly reduced aluminum content.
Claim 22
Dependent Claim
135 words
A process for preparing a soy protein isolate substantially free of phytate and with significantly reduced aluminum content which comprises in sequence the steps of: (a) forming an aqueous slurry of a defatted soybean raw material at a pH within the range of 8-10 at a temperature above 65° C. for a period of 1-15 minutes to solubilize the soy protein; (b) reducing the temperature to 25°-65° C. for a period of 10-60 minutes; (c) separating said slurry into an insoluble fraction and a soluble fraction which includes the solubilized soy protein; (d) adjusting the pH of said soluble fraction to pH 5.0 to 5.5 with a non-toxic water soluble acid at a temperature of 25°-65° C. thereby precipitating and separating said soy protein isolate substantially free of phytate and with significantly reduced aluminum content.
Claim 23
Dependent Claim
46 words
The process of claim 20 wherein step (a) is carried out at pH of 8.5 to 9.5 at 70°-85° C. for 1-15 minutes; step (b) is carried out at 50°-60° C. for 10-60 minutes; and step (d) is carried out at pH 5.2-5.4 at 50°-60° C.
Claim 24
Dependent Claim
43 words
The process of claim 20 wherein step (a) is carried out at pH 8.5-9.5 at 75°-80° C. for 2-5 minutes; step (b) is carried out at 55°-60° C. for 10-30 minutes; and step (d) is carried out at pH 5.3 at 50°-60° C.
Claim 25
Dependent Claim
31 words
The process of claim 1 wherein said soy protein solids fraction having a phytate concentration below 0.3% is washed in an aqueous media and then separated from the aqueous Wash media.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-09-28 | 1991-03-30 | 1991-09-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-09-28 | 1995-03-30 | 1995-09-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-09-28 | 1999-03-30 | 1999-09-29 | $7700.00 | Window Closed / Lapsed |