US4696901A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of microorganisms on a plastic carrier
Assignee: Gist Brocades NV
•
Filing: 1983-12-07
•
Granted: 1987-09-29
•
Expires: 2003-12-07
Abstract
Abstract Hydrocarbon utilizing microorganisms are immobilized on a plastic carrier in an aqueous nutrient medium to which a minor amount of a water-immiscible hydrocarbon substrate has been added. The water-immiscible hydrocarbon is preferably hexadecane or dodecylcyclohexane and the plastic carrier is preferably polytetrafluoroethylene. Presence of the water-immiscible hydrocarbon substrate results in increased loading of microorganisms on the carrier.
Inventors
- 👤 Brian W. Robertson
- 👤 Gareth T. Phillips
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:47
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12P1/00
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
54 words
In a process for the immobilisation of one or more hydrocarbon-utilising microorganisms on a plastic carrier in which the immobilisation is carried out in an aqueous nutrient medium, the improvement comprising adding to the nutrient medium from about 0.1-3 ml of a water-immiscible hydrocarbon substrate per 100 cm 2 of plastic carrier surface area.
Claim 2
Dependent Claim
54 words
In a process for the immobilisation of one or more hydrocarbon-utilising microorganisms on a plastic carrier in which the immobilisation is carried out in an aqueous nutrient medium, the improvement comprising adding to the nutrient medium from about 0.1-3 ml of a water-immiscible hydrocarbon substrate per 100 cm 2 of plastic carrier surface area.
Claim 3
Dependent Claim
19 words
Process as claimed in claim 1 wherein said plastic carrier does not contain plasticizer and is not plasma treated.
Claim 4
Dependent Claim
29 words
Process as claimed in claim 1 or 2 in which the water-immiscible hydrocarbon substrate is selected from the group consisting of alkanes having 12-18 carbon atoms and mixtures thereof.
Claim 5
Dependent Claim
14 words
Process as claimed in claim 3 in which the water-immiscible hydrocarbon substrate is hexadecane.
Claim 6
Dependent Claim
24 words
Process as claimed in claim 3 in which the immobilisation is carried out at a temperature which is in the range of 25°-37° C.
Claim 7
Dependent Claim
14 words
Process as claimed in claim 3 in which the water-immiscible hydrocarbon substrate is dodecylcyclohexane.
Claim 8
Dependent Claim
22 words
Process as claimed in claim 5 in which the plastic carrier is selected from the group consisting ofpolytetrafluoroethylene, nylon, polyethylene and polyvinylchloride.
Claim 9
Dependent Claim
13 words
Process as claimed in claim 7 in which the plastic carrier is polytetrafluoroethylene.
Claim 10
Dependent Claim
32 words
Process as claimed in claim 7 in which the hydrocarbon utilizing microorganism is selected from the group consisting of Mycobacterium rhodochrous 7E1C NCIB 9703, C. hydrocarboxydans ATCC 21761 and Pseudomonas aeruginosa 473.
Claim 11
Dependent Claim
23 words
Process as claimed in claim 7 in which the hydrocarbon--utilising microorganisms are selected from the genera consisting of Mycobacterium, Corynebacterium, Arthrobacter and Pseudomonas.
Claim 12
Dependent Claim
32 words
Process as claimed in claim 10 in which the hydrocarbon utilising microorganism is selected from the group consisting of Mycobacterium rhodochrous 7E1C NCIB 9703, Mycobacterium lacticolum NCIB 9739 and Pseudomonas aeruginosa 473.
Claim 13
Dependent Claim
13 words
Process as claimed in claim 7 in which the plastic carrier is polyethylene.
Claim 14
Dependent Claim
32 words
Process as claimed in claim 12 in which the hydrocarbon utilizing microorganism is selected from the group consisting of Mycobacterium rhodochrous 7E1C NCIB 9703, C. hydrocarboxydans ATCC 21761 and Pseudomonas aeruginosa 473.
References Cited (Backward Citations)
DE1924333A1
Cited
US3642577A
Cited
US3888736A
Cited
US4011135A
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US4332904A
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US4504547A
Cited
US4537790A
Cited
JPS568691A
Cited
BE884876A
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US4547463A
Cited
EP0112597A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-09-28 | 1991-03-30 | 1991-09-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-09-28 | 1995-03-30 | 1995-09-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-09-28 | 1999-03-30 | 1999-09-29 | $7700.00 | Window Closed / Lapsed |