US4695552A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process and reagent for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin
Assignee: Roche Diagnostics GmbH
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Filing: 1984-04-19
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Granted: 1987-09-22
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Expires: 2004-04-19
Abstract
Abstract The present invention provides a process for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin by utilization of the different peroxidate properties of free and of bound hemoglobin, wherein, for the selective inhibition of the peroxidase activity of the free hemoglobin, a detergent is added and the residual peroxidate activity of the reaction mixture is measured. The present invention also provides a reagent for carrying out this process wherein, besides the substances required for the determination of the peroxidase activity, it contains a detergent for the inhibition of the peroxidase activity of free hemoglobin. Furthermore, the present invention provides a process for determining the haptoglobin content of a sample, as well as a process for determining glycosilated hemoglobin in a sample.
Inventors
- 👤 Urban Schmitt
- 👤 Rolf Deeg
- 👤 Joachim Ziegenhorn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 14:25
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/725; C12Q2326/00; C12Q2326/30; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
73 words
A process for the determination of the hemoglobin-haptoglobin complex in a sample containing free hemoglobin comprising the steps of adding a detergent to the sample to be determined, in an effective amount to almost completely inactivate the peroxidase activity of the free hemoglobin without substantially changing the peroxidase activity of the hemoglobin-haptoglobin complex, and thereafter measuring the residual peroxidase activity of the reaction mixture as a measure of the amount of hemoglobin-haptoglobin complex.
Claim 2
Dependent Claim
73 words
A process for the determination of the hemoglobin-haptoglobin complex in a sample containing free hemoglobin comprising the steps of adding a detergent to the sample to be determined, in an effective amount to almost completely inactivate the peroxidase activity of the free hemoglobin without substantially changing the peroxidase activity of the hemoglobin-haptoglobin complex, and thereafter measuring the residual peroxidase activity of the reaction mixture as a measure of the amount of hemoglobin-haptoglobin complex.
Claim 3
Dependent Claim
24 words
The process of claim 1, wherein the detergent is selected from the group consisting of the saponins, lanolins and detergents with a sugar component.
Claim 4
Dependent Claim
16 words
The process of claim 2, wherein saponin, digitonin or polyoxyethylene-sorbitol detergent is used as the detergent.
Claim 5
Dependent Claim
13 words
The process of claim 2 wherein 0.1 to 50 grams/liter detergent is used.
Claim 6
Dependent Claim
13 words
The process of claim 1 wherein 0.1 to 50 grams/liter detergent is used.
Claim 7
Dependent Claim
21 words
The process of claim 1 wherein a peroxide and a chromogenic substrate is used for the measurement of the peroxidase activity.
Claim 8
Dependent Claim
16 words
Process according to claim 6, wherein guaiacol or 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonic acid is used as the chromogenic substrate.
Claim 9
Dependent Claim
25 words
Process according to claim 6, wherein said chromogenic substrate is 4-aminoantipyrine, 3-methyl-2-benzthiazolone hydrazone or 3-methyl-2-benzthiazolone-hydrazone sulphonic acid, in combination with a phenol or aniline derivative.
Claim 10
Dependent Claim
32 words
A process for the determination of haptoglobin content of a sample, comprising mixing the sample with an excess of hemoglobin, and thereafter determining the hemoglobin-haptoglobin complex by the process of claim 1.
Claim 11
Dependent Claim
39 words
Process for the determination of glycosylated hemoglobin in a sample by differentiation of glycosylated and non-glycosylated hemoglobin with the use of haptoglobin and thereafter measuring the peroxidase activity of the glycosylated hemoglobin-haptoglobin complex with the process of claim 1.
Claim 12
Dependent Claim
78 words
A reagent for determining the hemoglobin-haptoglobin complex in the presence of free hemoglobin comprising means for the determination of the peroxidase activity of said hemoglobin-haptoglobin complex and a detergent selected from the group consisting of saponins and lanolins for the inhibition of the peroxidase activity of free hemoglobin, wherein said detergent is present in an amount effective to almost completely inactivate the peroxidase activity of said free hemoglobin without substantially changing the peroxidase activity of said hemoglobin-haptoglobin complex.
Claim 13
Dependent Claim
78 words
A reagent for determining the hemoglobin-haptoglobin complex in the presence of free hemoglobin comprising means for the determination of the peroxidase activity of said hemoglobin-haptoglobin complex and a detergent selected from the group consisting of saponins and lanolins for the inhibition of the peroxidase activity of free hemoglobin, wherein said detergent is present in an amount effective to almost completely inactivate the peroxidase activity of said free hemoglobin without substantially changing the peroxidase activity of said hemoglobin-haptoglobin complex.
Claim 14
Dependent Claim
14 words
The reagent of claim 11, wherein saponin or digitonin is used as the detergent.
Claim 15
Dependent Claim
19 words
The reagent of claim 11, containing 0.1-50 g./liter detergent 0.1-100 mmol/liter peroxide 0.1-5 mmol/liter chromogenic substrate 0.001-0.5 mol/liter buffer.
Claim 16
Dependent Claim
22 words
The reagent of claim 13, containing 1-5 g./liter saponin 0.5-25 mmol/liter perborate 0.5-2 mmol/liter 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) 0.01-0.2 mol/liter citrate buffer, pH 3.5-4.5.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-09-21 | 1991-03-23 | 1991-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-09-21 | 1995-03-23 | 1995-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-09-21 | 1999-03-23 | 1999-09-22 | $7700.00 | Window Closed / Lapsed |