US4695548A
Expired / Lapsed
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Gel inserts useful in electrophoresis
Assignee: Columbia University
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Filing: 1984-09-25
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Granted: 1987-09-22
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Expires: 2004-09-25
Abstract
Abstract Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.
Inventors
- 👤 Charles R. Cantor
- 👤 David C. Schwartz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 17:46
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44773; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2984; Y10T428/2985; Y10T428/2987
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
42 words
A gel insert useful for electrophoretic separations which comprises a gel matrix consisting of a solidified agarose or acrylamide suitable for use in an electrophoretic method and entrapped within the gel matrix are lysed cells and macromolecules derived from the lysed cells.
Claim 2
Dependent Claim
42 words
A gel insert useful for electrophoretic separations which comprises a gel matrix consisting of a solidified agarose or acrylamide suitable for use in an electrophoretic method and entrapped within the gel matrix are lysed cells and macromolecules derived from the lysed cells.
Claim 3
Dependent Claim
11 words
A gel insert of claim 1, wherein the macromolecules are DNA.
Claim 4
Dependent Claim
12 words
A gel insert of claim 1, wherein the macromolecules are intact chromosomes.
Claim 5
Dependent Claim
13 words
A gel insert of claim 1, wherein the lysed cells are mammalian cells.
Claim 6
Dependent Claim
79 words
A method of preparing a gel insert of claim 1 which comprises suspending cells in a liquid agarose or acrylamide suitable for use in an electrophoretic method, allowing the agarose or acrylamide to form a gel matrix in which the suspended cells are entrapped, diffusing a cell lysing reagent into the gel matrix containing the suspended cells so as to lyse the cells, and yield lysed cells and macromolecules derived from the lysed cells entrapped in the gel matrix.
Claim 7
Dependent Claim
33 words
A method of claim 5, wherein the gel inserts are formed in a predetermined size and shape in a mold by adding the cells and the liquid to the mold in measured amounts.
Claim 8
Dependent Claim
16 words
A method of claim 6, wherein the addition of the cells and the liquid is automated.
References Cited (Backward Citations)
US4391688A
Cited
US4473452A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-09-21 | 1991-03-23 | 1991-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-09-21 | 1995-03-23 | 1995-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-09-21 | 1999-03-23 | 1999-09-22 | $7700.00 | Window Closed / Lapsed |