US4690905A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for removal of human antibodies to native DNA from serum
Assignee: Albert Einstein College of Medicine
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Filing: 1983-11-30
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Granted: 1987-09-01
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Expires: 2003-11-30
Abstract
Abstract The invention relates to monoclonal anti-idiotypic antibodies to human anti-DNA antibodies. Monoclonal, anti-idiotypic antibodies are produced using hybridoma technology. The antibodies are used as diagnostic reagents in methods to determine the presence of anti-native DNA antibodies in serum from patients suspected of having systemic lupus erythematosus, and as therapeutic reagents in methods to remove the anti-native DNA antibodies from the serum of patients with systemic lupus erythematosus.
Inventors
- 👤 Betty A. Diamond
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/577; C; C07; C07K; C07K16/00; C07K16/42; C07K16/4208; C07K16/4241; G01N33/564; G01N33/68; G01N33/6854; G01N33/686; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/824; Y10S530/00; Y10S530/808; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/868
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
58 words
A method for removing human anti-native DNA antibody from a liquid sample comprising contacting a liquid sample with a medium containing monoclonal, anti-idiotypic antibodies capable of binding to a shared idiotype on human anti-native DNA antibody, said idiotype shared between genetically nonidentical individuals, and separating said medium from said sample to remove the human anti-native DNA antibodies therefrom.
Claim 2
Independent Claim
58 words
A method for removing human anti-native DNA antibody from a liquid sample comprising contacting a liquid sample with a medium containing monoclonal, anti-idiotypic antibodies capable of binding to a shared idiotype on human anti-native DNA antibody, said idiotype shared between genetically nonidentical individuals, and separating said medium from said sample to remove the human anti-native DNA antibodies therefrom.
Claim 3
Dependent Claim
37 words
A method as in claim 1 in which said monoclonal antibodies are capable of reacting with a shared idiotype on human anti-native DNA antibody which is located outside the DNA binding site of the anti-native DNA antibody.
Claim 4
Independent Claim
76 words
A method for removing human anti-native DNA antibody from a liquid sample comprising coupling monoclonal, anti-idiotypic antibodies capable of binding to a shared idiotype on human anti-native DNA antibody to a medium, said idiotype shared between genetically nonidentical individuals, contacting a liquid sample to said medium to permit binding of human anti-native DNA antibody in said sample to said anti-idiotypic antibodies and separating said sample from said medium to remove the human anti-native DNA antibodies therefrom.
Claim 5
Independent Claim
76 words
A method for removing human anti-native DNA antibody from a liquid sample comprising coupling monoclonal, anti-idiotypic antibodies capable of binding to a shared idiotype on human anti-native DNA antibody to a medium, said idiotype shared between genetically nonidentical individuals, contacting a liquid sample to said medium to permit binding of human anti-native DNA antibody in said sample to said anti-idiotypic antibodies and separating said sample from said medium to remove the human anti-native DNA antibodies therefrom.
Claim 6
Dependent Claim
24 words
A method as in claim 3 which further comprises eluting the bound anti-native DNA antibodies from said medium to permit reuse of said medium.
Claim 7
Dependent Claim
37 words
A method as in claim 3 in which said monoclonal antibodies are capable of reacting with a shared idiotype on human anti-native DNA antibody which is located outside the DNA binding site of the anti-native DNA antibody.
Claim 8
Dependent Claim
14 words
A method as in claim 3 in which said medium is a solid phase.
Claim 9
Dependent Claim
12 words
A method as in claim 6 in which said medium comprises sepharose.
Claim 10
Dependent Claim
24 words
A method as in claim 7 in which said monoclonal, anti-idiotypic antibody is coupled to said sepharose by treating said sepharose with cyanogen bromide.
Claim 11
Dependent Claim
30 words
A method as in claim 3 in which said monoclonal, anti-idiotypic antibodies are secreted by the hybridoma deposited at the American Type Culture Collection and catalogued as ATCC #HB 8376.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-08-31 | 1991-03-02 | 1991-09-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-08-31 | 1995-03-02 | 1995-09-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-08-31 | 1999-03-02 | 1999-09-01 | $7700.00 | Window Closed / Lapsed |