US4687747A
Expired / Lapsed
US
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Phenanthridinium ester as a labelling compound in luminometric immunoassay
Assignee: BYK-Sangtec Diagnostica GmbH and Co KG
•
Filing: 1984-07-02
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Granted: 1987-08-18
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Expires: 2004-07-02
Abstract
Abstract Disclosed are novel chemiluminescent compounds and their method of use in luminometric immunoassays. The compounds, comprising salts of phenanthridinium esters, are particularly useful in sandwich immunoassays.
Inventors
- 👤 Wayne H. T. Lin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 13:18
CPC Classifications
C; C09; C09K; C09K11/00; C09K11/06; C09K11/07; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
50 words
A chemiluminescent compound of the formula: ##STR7## where R is an alkyl group having from 1 to 5 carbon atoms, X is an anion selected from the group consisting of sulfates, alkylsulfates, halosulfonates and halides, and Y is a chemical moiety which may bind to groups commonly found in analytes.
Claim 2
Dependent Claim
50 words
A chemiluminescent compound of the formula: ##STR7## where R is an alkyl group having from 1 to 5 carbon atoms, X is an anion selected from the group consisting of sulfates, alkylsulfates, halosulfonates and halides, and Y is a chemical moiety which may bind to groups commonly found in analytes.
Claim 3
Dependent Claim
21 words
A chemiluminescent compound as in claim 1, wherein the compound is: ##STR8## where n is an integer from 0 to 5.
Claim 4
Dependent Claim
12 words
A chemiluminescent compound as in claim 2, wherein the compound is: ##STR9##
Claim 5
Dependent Claim
12 words
A chemiluminescent compound as in claim 2, wherein the compound is: ##STR10##
Claim 6
Dependent Claim
20 words
A chemiluminescent reagent useful in a luminometric immunoassay, comprising a conjugate of the compound of claim 1 with an antibody.
Claim 7
Dependent Claim
20 words
A chemiluminescent reagent useful in a luminometric immunoassay, comprising a conjugate of the compound of claim 1 with an antigen.
Claim 8
Dependent Claim
135 words
A luminometric immunoassay for detection of an antigen in a sample, comprising: incubating an immobilized antibody specific to the antigen with a sample solution and a solution of labeled antibody; and forming thereby an immobilized sandwich, said labeled antibody comprising a conjugate of an antibody and a chemiluminescent compound of the formula: ##STR11## where R is an alkyl group having from 1 to 5 carbon atoms, X is an anion selected from the group consisting of sulfates, alkylsulfates, halosulfonates and halides, and Y is a chemical moiety which may bind to groups commonly found in analytes; separating the solution of labeled antibody from the immobilized sandwich; reacting the labeled antibody in the immobilized sandwich with an oxidizing agent to cause light emission; and measuring the amount of light emitted to determine the presence of antigen.
Claim 9
Dependent Claim
135 words
A luminometric immunoassay for detection of an antigen in a sample, comprising: incubating an immobilized antibody specific to the antigen with a sample solution and a solution of labeled antibody; and forming thereby an immobilized sandwich, said labeled antibody comprising a conjugate of an antibody and a chemiluminescent compound of the formula: ##STR11## where R is an alkyl group having from 1 to 5 carbon atoms, X is an anion selected from the group consisting of sulfates, alkylsulfates, halosulfonates and halides, and Y is a chemical moiety which may bind to groups commonly found in analytes; separating the solution of labeled antibody from the immobilized sandwich; reacting the labeled antibody in the immobilized sandwich with an oxidizing agent to cause light emission; and measuring the amount of light emitted to determine the presence of antigen.
Claim 10
Dependent Claim
26 words
A luminometric immunoassay as in claim 7, wherein, prior to addition of the solution of labeled antibody, the sample solution is separated from the immobilized antibody.
Claim 11
Dependent Claim
117 words
A luminometric immunoassay for detection of an antigen in a sample, comprising: incubating an immobilized antibody with a sample solution and a labeled antigen, said labeled antigen comprising a conjugate of the antigen and a chemiluminescent compound having the formula: ##STR12## where R is an alkyl group having from 1 to 5 carbon atoms, X is an anion selected from the group consisting of sulfates, alkylsulfates, halosulfonates and halides, and Y is a chemical moiety which may bind to groups commonly found in analytes; separating bound antigen from unbound antigen; reacting the bound labeled antigen with an oxidizing agent to cause light emission; and measuring the amount of light emitted to determine the presence of unlabeled antigen.
Claim 12
Dependent Claim
117 words
A luminometric immunoassay for detection of an antigen in a sample, comprising: incubating an immobilized antibody with a sample solution and a labeled antigen, said labeled antigen comprising a conjugate of the antigen and a chemiluminescent compound having the formula: ##STR12## where R is an alkyl group having from 1 to 5 carbon atoms, X is an anion selected from the group consisting of sulfates, alkylsulfates, halosulfonates and halides, and Y is a chemical moiety which may bind to groups commonly found in analytes; separating bound antigen from unbound antigen; reacting the bound labeled antigen with an oxidizing agent to cause light emission; and measuring the amount of light emitted to determine the presence of unlabeled antigen.
Claim 13
Dependent Claim
24 words
A luminometric immunoassay as in claim 7 or 8, wherein the chemiluminescent compound is: ##STR13## where n is an integer from 0 to 5.
Claim 14
Dependent Claim
13 words
A luminometric immunoassay as in claim 10, wherein the chemiluminescent compound is: ##STR14##
Claim 15
Dependent Claim
13 words
A luminometric immunoassay as in claim 10, wherein the chemiluminescent compound is ##STR15##
Claim 16
Dependent Claim
22 words
A luminometric immunoassay as in claim 9, wherein the chemiluminescent compound is ##STR16## where n is an integer from 0 to 5.
Claim 17
Dependent Claim
13 words
A luminometric immunoassay as in claim 13 wherein the chemiluminescent compound is ##STR17##
Claim 18
Dependent Claim
13 words
A luminometric immunoassay as in claim 13, wherein the chemiluminescent compound is ##STR18##
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-08-17 | 1991-02-16 | 1991-08-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-08-17 | 1995-02-16 | 1995-08-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-08-17 | 1999-02-16 | 1999-08-18 | $7700.00 | Window Closed / Lapsed |