US4681848A
Expired / Lapsed
US
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Novel peptide and use thereof
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1983-09-20
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Granted: 1987-07-21
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Expires: 2003-09-20
Abstract
Abstract The invention relates to novel polypeptide of the formula ##STR1## wherein X is a bond, or a peptide or amino acid residue 1 to 16 amino acids counting from the C terminus of the peptide chain of ##STR2## and Y is a peptide or amino acid residue having 1 to 5 amino acids counting from the N terminus of the peptide chain of ##STR3## and conjugate between the same and a carrier protein, as well as hydridoma and monoclonal antibody derived by the use of the polypeptide or the conjugate, and a method of detecting and of purifying human gamma-interferon using the antibodies.
Inventors
- 👤 Kyozo Tsukamoto
- 👤 Yuzo Ichimori
- 👤 Mitsuhiro Wakimasu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 09:52
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/57; C07K16/00; C07K16/18; C07K16/24; C07K16/249; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6863; G01N33/6866; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S530/00; Y10S530/806; Y10S530/808; Y10S530/809; Y10S530/861; Y10S930/00; Y10S930/01; Y10S930/14; Y10S930/142
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
102 words
A cloned hybridoma between (a) a spleen cell of a mammal immunized with a polypeptide of the formula: ##STR10## wherein X is a bond, or a peptide or amino acid residue having 1 to 16 amino acids counting from the C terminus of the peptide chain of ##STR11## and Y is a peptide or amino acid residue having 1 to 5 amino acids counting from the N terminus of the peptide chain of ##STR12## or with a conjugate between polypeptide (I) and a carrier protein and (b) lymphoid cell from a mammal of the same or different species from the immunized mammal.
Claim 2
Dependent Claim
102 words
A cloned hybridoma between (a) a spleen cell of a mammal immunized with a polypeptide of the formula: ##STR10## wherein X is a bond, or a peptide or amino acid residue having 1 to 16 amino acids counting from the C terminus of the peptide chain of ##STR11## and Y is a peptide or amino acid residue having 1 to 5 amino acids counting from the N terminus of the peptide chain of ##STR12## or with a conjugate between polypeptide (I) and a carrier protein and (b) lymphoid cell from a mammal of the same or different species from the immunized mammal.
Claim 3
Dependent Claim
11 words
The hybridoma of claim 1, wherein the mammal is a mouse.
Claim 4
Dependent Claim
13 words
The hybridoma of claim 1, wherein the lymphoid cell is a myeloma cell.
Claim 5
Dependent Claim
65 words
A monoclonal antibody against a polypeptide of the formula: ##STR13## wherein X is a bond, or a peptide or amino acid residue having 1 to 16 amino acids counting from the C terminus of the peptide chain of ##STR14## and Y is a peptide or amino acid residue having 1 to 5 amino acids counting from the N terminus of the peptide chain of ##STR15##
Claim 6
Dependent Claim
65 words
A monoclonal antibody against a polypeptide of the formula: ##STR13## wherein X is a bond, or a peptide or amino acid residue having 1 to 16 amino acids counting from the C terminus of the peptide chain of ##STR14## and Y is a peptide or amino acid residue having 1 to 5 amino acids counting from the N terminus of the peptide chain of ##STR15##
Claim 7
Dependent Claim
14 words
The monoclonal antibody of claim 4, which is capable of binding to human gamma-interferon.
Claim 8
Dependent Claim
18 words
The monoclonal antibody of claim 4, which belongs to the subclass IgG2b, when tested by the Ouchterlony method.
Claim 9
Dependent Claim
18 words
The monoclonal antibody of claim 4, which belongs to the subclass IgG1, when tested by the Ouchterlony method.
Claim 10
Dependent Claim
16 words
Monoclonal antibodies to human gamma-interferon protein, produced by hybridoma cell line ATCC HB8699 or ATCC HB8700.
Claim 11
Dependent Claim
16 words
Monoclonal antibodies to human gamma-interferon protein, produced by hybridoma cell line ATCC HB8699 or ATCC HB8700.
Claim 12
Dependent Claim
28 words
A hybridoma cell which produces a monoclonal antibody to human gamma-interferon, the cell being from the hybridoma cell line ATCC HB8699 or the hybridoma cell line ATCC HB8700.
Claim 13
Dependent Claim
28 words
A hybridoma cell which produces a monoclonal antibody to human gamma-interferon, the cell being from the hybridoma cell line ATCC HB8699 or the hybridoma cell line ATCC HB8700.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-07-20 | 1991-01-19 | 1991-07-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-07-20 | 1995-01-19 | 1995-07-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-07-20 | 1999-01-19 | 1999-07-21 | $7700.00 | Window Closed / Lapsed |