US4680201A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Coating for electrophoresis tube
Assignee: Bio Rad Laboratories Inc
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Filing: 1985-10-30
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Granted: 1987-07-14
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Expires: 2005-10-30
Abstract
Abstract Coated capillary tubes useful for performing free electrophoretic separation techniques are prepared by reacting the interior wall of the tube with a bifunctional compound having a first functional group capable of covalently attaching to the wall and a second functional group capable of being polymerized. After such covalent attachment, free monomer is reacted with the second functional group, resulting in the formation of a monomolecular polymeric layer covalently attached to the interior wall of the capillary tube. This polymeric layer inhibits electroendosmosis and adsorption of the substances to be separated onto the interior of the tube, providing for improved separation.
Inventors
- 👤 Stellan Hjerten
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 11:04
CPC Classifications
B; B05; B05D; B05D7/00; B05D7/22; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44752
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
100 words
A method for preparing a thin-wall, narrow-bore capillary tube for use in electrophoretic separation, said method comprising: contacting a solution of a monomeric bifunctional compound with the inside of the tube, where the bifunctional compound includes a first functional group capable of covalent attachment to the tube wall and a second functional group capable of polymerization to form a monomolecular coating; washing the tube free of non-bound monomeric bifunctional compound; and introducing free monomer to the inside of the tube in order to react the covalently bound monomeric second functional group with free monomer to form a monomolecular polymeric coating.
Claim 2
Independent Claim
100 words
A method for preparing a thin-wall, narrow-bore capillary tube for use in electrophoretic separation, said method comprising: contacting a solution of a monomeric bifunctional compound with the inside of the tube, where the bifunctional compound includes a first functional group capable of covalent attachment to the tube wall and a second functional group capable of polymerization to form a monomolecular coating; washing the tube free of non-bound monomeric bifunctional compound; and introducing free monomer to the inside of the tube in order to react the covalently bound monomeric second functional group with free monomer to form a monomolecular polymeric coating.
Claim 3
Dependent Claim
23 words
A method as in claim 1, wherein the bifunctional compound is selected from the group consisting of γ-methacryloxypropyltrimethoxysilane, vinyltriacetoxysilane, vinyltri(β-methoxyethoxy)silane, vinyltrichlorosilane, and methylvinyldichlorosilane.
Claim 4
Dependent Claim
24 words
A method as in claim 2, wherein the free monomer is selected from the group consisting of acryl, acryloyl, methacryl, allyl, and vinyl monomers.
Claim 5
Dependent Claim
16 words
A method as in claim 1, wherein the tube is complsed of glass, quartz, or plastic.
Claim 6
Dependent Claim
13 words
A thin-wall, narrow-bore capillary tube prepared according to the method of claim 1.
Claim 7
Independent Claim
79 words
A method for preparing a thin-wall, narrow-bore capillary tube for use in electrophoresis separation, said method comprising: contacting the inside wall of the tube with an aqueous solution of γ-methacryloxypropyltrimethoxysilane (MPTS) at a pH below about 4.0; washing the wall substantially free from non-bound MPTS; and contacting the inside wall of the tube with an aqueous acrylamide solution capable of reacting with the MPTS to form a covalently bound monomolecular polyacrylamide layer on the inside wall of the tube.
Claim 8
Independent Claim
79 words
A method for preparing a thin-wall, narrow-bore capillary tube for use in electrophoresis separation, said method comprising: contacting the inside wall of the tube with an aqueous solution of γ-methacryloxypropyltrimethoxysilane (MPTS) at a pH below about 4.0; washing the wall substantially free from non-bound MPTS; and contacting the inside wall of the tube with an aqueous acrylamide solution capable of reacting with the MPTS to form a covalently bound monomolecular polyacrylamide layer on the inside wall of the tube.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-07-13 | 1991-01-12 | 1991-07-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-07-13 | 1995-01-12 | 1995-07-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-07-13 | 1999-01-12 | 1999-07-14 | $7700.00 | Window Closed / Lapsed |