US4676983A
Expired / Lapsed
US
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Tumor cytostatic-citocidal factor from blood platelets
Assignee: Japan Chemical Research Co Ltd
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Filing: 1986-03-21
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Granted: 1987-06-30
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Expires: 2006-03-21
Abstract
Abstract A tumor cytostatic-cytocidal factor is produced by disrupting platelets, extracting the disrupted platelets with an acidic aqueous solution or an acidic water-organic solvent mixture, and, after gel filtration, if desired, to separate a fraction having an estimated molecular weight of 10,000 to 20,000 and having tumor cell growth inhibiting activity, subjecting said extract or said fraction to chromatography to isolate a cytostatic fraction having a molecular weight of 10,000 to 20,000 and an isoelectric point of 9.0 to 10.0, giving a positive nihidrin reaction and being such that it is deactivated by trypsin but not deactivated by heating in physiological saline solution at 60° C. for 30 minutes or contacting at room temperature with 0.2N hydrochloric acid for 60 minutes or 70% formic acid for 30 minutes.
Inventors
- 👤 Toyohiko Nishimura
- 👤 Hajime Hiratani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 16:38
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/19; A61P; A61P35/00
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
138 words
A method of producing a tumor cytostatic-cytocidal factor characterized by disrupting platelets, extracting the disrupted platelets with an acidic aqueous solution or an acidic water-organic solvent mixture, and either directly thereafter or after gel filtration separating a fraction having an estimated molecular weight of 10,000 to 20,000 and having tumor cell growth inhibiting activity, subjecting said extract or said fraction as the case may be to chromatography to isolate a cytostatic fraction having a molecular weight of 10,000 to 20,000 and an isoelectric point of 9.0 to 10.0, giving a positive ninhydrin reaction and being such that it is deactivated by trypsin but is not deactivated by heating in physiological saline solution at 60° C. for 30 minutes or contacting at room temperature with 0.2 N hydrochloric acid for 60 minutes or 70% formic acid for 30 minutes.
Claim 2
Independent Claim
138 words
A method of producing a tumor cytostatic-cytocidal factor characterized by disrupting platelets, extracting the disrupted platelets with an acidic aqueous solution or an acidic water-organic solvent mixture, and either directly thereafter or after gel filtration separating a fraction having an estimated molecular weight of 10,000 to 20,000 and having tumor cell growth inhibiting activity, subjecting said extract or said fraction as the case may be to chromatography to isolate a cytostatic fraction having a molecular weight of 10,000 to 20,000 and an isoelectric point of 9.0 to 10.0, giving a positive ninhydrin reaction and being such that it is deactivated by trypsin but is not deactivated by heating in physiological saline solution at 60° C. for 30 minutes or contacting at room temperature with 0.2 N hydrochloric acid for 60 minutes or 70% formic acid for 30 minutes.
Claim 3
Dependent Claim
19 words
A method as set forth in claim 1 wherein said gel chromatography is performed under acidic to neutral conditions.
Claim 4
Dependent Claim
101 words
A method as set forth in claim 1 wherein said chromatography is (1) ion exchange chromatography which comprises adsorbing the activity on a cation exchanger and, then, carrying out gradient elution with an aqueous solution system of a neutral salt, (2) reversed-phase chromatography which comprises adsorbing said activity on a support carrying hydrophobic groups and, then, carying out gradient elution using a hydrophilic neutral organic solvent system or (3) chromatofocusing which comprises adsorbing said activity on a cation exchanger and, then, carrying out elution isoelectrically using a pH-gradient eluant system or a combination of two or more of the chromatographic procedures.
Claim 5
Dependent Claim
11 words
A tumor cytostatic-cytocidal factor prepared by the method of claim 1.
Claim 6
Dependent Claim
79 words
A tumor cytostatic-cytocidal factor obtained from blood platelet extract, having a molecular weight of from 10,000 to 20,000 as determined by gel filtration, an isoelectric point of 9.0 to 10.0, which factor gives a positive ninhydrin reaction, and which is deactivated by trypsin but not deactivated by heating in physiological salt solution at 60° C. over 30 minutes or upon contact with 0.2 N hydrochloric acid over 60 minutes or 70% formic acid over 30 minutes at room temperature.
Claim 7
Dependent Claim
79 words
A tumor cytostatic-cytocidal factor obtained from blood platelet extract, having a molecular weight of from 10,000 to 20,000 as determined by gel filtration, an isoelectric point of 9.0 to 10.0, which factor gives a positive ninhydrin reaction, and which is deactivated by trypsin but not deactivated by heating in physiological salt solution at 60° C. over 30 minutes or upon contact with 0.2 N hydrochloric acid over 60 minutes or 70% formic acid over 30 minutes at room temperature.
Claim 8
Dependent Claim
25 words
A pharmaceutical composition useful for the treatment of tumors comprised of an anti-tumor effective amount cytostatic-cytocidal factor of claim 5, and a pharmaceutically acceptable carrier.
Claim 9
Dependent Claim
22 words
A method of producing an anti-tumor affect comprising administering to tumor cells an effective amount of the pharmaceutical composition of claim 6.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-29 | 1990-12-29 | 1991-06-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-29 | 1994-12-29 | 1995-06-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-29 | 1998-12-29 | 1999-06-30 | $7700.00 | Window Closed / Lapsed |