US4675384A
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Fractionation/purification of plasma by ion exchange chromatography
Assignee: Rhone Poulenc Recherches SA
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Filing: 1984-04-02
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Granted: 1987-06-23
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Expires: 2004-04-02
Abstract
Abstract The plasma proteins contained in a plasma solution thereof are chromatographically fractioned, e.g., to obtain a high purity therapeutic grade albumin product, by successively contacting said plasma solution with at least one anion exchanger and at least one cation exchanger in fixed bed chromatography columns, the contact and exchange medium of chromatography therein also comprising a plurality of at least partially hydrophobic support particulates and a plurality of hydrophilic ion exchanger support particulates.
Inventors
- 👤 Adrien Dromard
- 👤 Michel Exertier
- 👤 Claude Rollin
- 👤 Jean-Louis Tayot
- 👤 Michel Tardy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 23:46
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/06; C07K16/065; B; B01; B01J; B01J39/00; B01J39/08; B01J39/16; B01J39/17; B01J47/00; B01J47/014; C07K14/00; C07K14/435; C07K14/76; C07K14/765; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
69 words
In a process for the chromatographic fractionation of the plasma proteins contained in a plasma solution thereof by successively contacting said plasma solution with at least one anion exchanger and at least one cation exchanger, the improvement which comprises contacting said plasma solution with a contact and exchange chromatography medium comprising a plurality of at least partially hydrophobic support particulates and a plurality of hydrophilic ion exchanger support particulates.
Claim 2
Dependent Claim
69 words
In a process for the chromatographic fractionation of the plasma proteins contained in a plasma solution thereof by successively contacting said plasma solution with at least one anion exchanger and at least one cation exchanger, the improvement which comprises contacting said plasma solution with a contact and exchange chromatography medium comprising a plurality of at least partially hydrophobic support particulates and a plurality of hydrophilic ion exchanger support particulates.
Claim 3
Dependent Claim
18 words
The process as defined by claim 1, said at least partially hydrophobic support particulates themselves comprising ion exchangers.
Claim 4
Dependent Claim
19 words
The process as defined by claim 1, said at least partially hydrophobic support particulates being incapable of ion exchange.
Claim 5
Dependent Claim
25 words
The process as defined by claim 2, said at least partially hydrophobic support particulates comprising anion exchangers and said hydrophilic support particulates comprising cation exchangers.
Claim 6
Dependent Claim
14 words
The process as defined by claim 1, said hydrophilic support particulates comprising cation exchangers.
Claim 7
Dependent Claim
28 words
The process as defined by claim 4, said at least partially hydrophobic support particulates comprising anion exchangers and said hydrophilic support particulates comprising both anion and cation exchangers.
Claim 8
Dependent Claim
33 words
The process as defined by claim 6, wherein the plasma solution is contacted with the hydrophilic anion exchanger support particulates upstream of contact with the at least partially hydrophobic anion exchanger support particulates.
Claim 9
Dependent Claim
35 words
The process as defined by claim 1, said support particulates each comprising a natural polysaccharide polymer, a synthetic polymer, inorganic oxides coated with a polysaccharide polymer, or inorganic oxides coated with a cross-linked synthetic polymer.
Claim 10
Dependent Claim
44 words
The process as defined by claim 1, said at least partially hydrophobic support particulates comprising a polysaccharide polymer including hydrophobic moieties, a synthetic polymer adsorbent, inorganic oxides coated with a polysaccharide polymer including hydrophobic moieties, or inorganic oxides coated with a cross-linked hydrophobic polymer.
Claim 11
Dependent Claim
38 words
The process as defined by claim 1, said hydrophilic ion exchanger support particulates comprising a polysaccharide polymer, a water-insoluble synthetic hydrophilic polymer, inorganic oxides coated with a polysaccharide polymer, or inorganic oxides coated with a cross-linked hydrophilic polymer.
Claim 12
Dependent Claim
57 words
The process as defined by claim 1, said at least partially hydrophobic support particulates and said hydrophilic ion exchanger support particulates comprising porous inorganic oxides having a grain size distribution ranging from 4 μm to 5 mm, pore diameters ranging from 250 to 3,000 Å, and a specific surface ranging from 5 to 150 m 2 /g.
Claim 13
Dependent Claim
39 words
The process as defined by claim 11, said grain size distribution ranging from 50 μm to 1 mm, said pore diameters ranging from 600 to 1,500 Å, and said specific surface ranging from 20 to 50 m 2 /g.
Claim 14
Dependent Claim
68 words
The process as defined by claim 1, said at least one anion exchanger and said at least one cation exchanger having an ion exchange capacity of less than about 2 meq/g and comprising porous inorganic particulates having a grain size distribution ranging from 4 μm to 5 mm, pore diameters ranging from 500 to 2,500 Å, and a specific surface ranging from 5 to 150 m 2 /g.
Claim 15
Dependent Claim
63 words
The process as defined by claim 13, porous inorganic particulates coated with less than about 15 mg/m 2 of a cross-linked hydrophobic polymer including amine or quaternary ammonium salt functions comprising at least one of said ion exchangers, and porous inorganic particulates coated with less than about 15 mg/m 2 of a cross-linked polyvinyllactam polymer bearing carboxylic acid functions comprising another ion exchanger.
Claim 16
Dependent Claim
70 words
The process as defined by claim 1, further comprising contacting said plasma solution with porous inorganic particulates having a grain size distribution ranging from 4 μm to 5 mm, pore diameters ranging from 500 to 2,500 Å, and a specific surface ranging from 5 to 150 m 2 /g, said particulates being coated with less than 15 mg/m 2 of a polysaccharide polymer bearing amine or quaternary ammonium salt functions.
Claim 17
Dependent Claim
41 words
The process as defined by claim 14, at least one cation exchanger comprising the polyvinyllactam polymer coated particulates, said polymer being prepared by copolymerization of a vinyllactam and an unsaturated carboxylic acid comonomer in the presence of a polyfunctional cross-linking agent.
Claim 18
Dependent Claim
11 words
The process as defined by claim 16, said vinyllactam comprising N-vinylpyrrolidone.
Claim 19
Dependent Claim
15 words
The process as defined by claim 16, said unsaturated carboxylic acid comonomer comprising acrylic acid.
Claim 20
Dependent Claim
42 words
The process as defined by claim 16, said cross-linking agent comprising a silane derivative of the formula CH 2 ═CH--SiX 3 , wherein each x, which may be identical or different, is hydrolyzable lower alkoxy, acetoxy or phenoxy radical, or a halogen.
Claim 21
Dependent Claim
24 words
The process as defined by claim 14, at least one anion exchanger comprising particulates coated with a hydrophobic cross-linked polymer prepared from vinylaromatic monomers.
Claim 22
Dependent Claim
31 words
The process as defined by claim 1, comprising employing from 250 to 2,000 ml of anion exchangers and 200 to 1,000 ml of cation exchangers per liter of plasma solution contacted.
Claim 23
Dependent Claim
40 words
The process as defined by claim 1, said chromatographic fractionation being carried out continuously and successively in a series of chromatography columns packed with said anion and cation exchangers and said at least partially hydrophobic and said hydrophilic support particulates.
Claim 24
Dependent Claim
17 words
The process as defined by claim 1, wherein albumin is selectively fractioned from the other plasma proteins.
Claim 25
Dependent Claim
16 words
The process as defined by claim 1, said beginning plasma solution being at least partially desalinized.
Claim 26
Dependent Claim
19 words
An at least 99% pure albumin, as determined by electrophoresis, prepared by the process as defined by claim 1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-22 | 1990-12-22 | 1991-06-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-22 | 1994-12-22 | 1995-06-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-22 | 1998-12-22 | 1999-06-23 | $7700.00 | Window Closed / Lapsed |