US4661705A
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US
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Quench, volume and activity determinations in a liquid scintillation flow system
Assignee: Beckman Coulter Inc
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Filing: 1984-05-21
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Granted: 1987-04-28
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Expires: 2004-05-21
Abstract
Abstract A method for use with liquid scintillation flow systems including flowing a mixture comprising a sample and a liquid scintillation medium through a flow detector, the liquid scintillation medium including a radionuclide marker having an energy distribution different from the sample. A pulse height distribution spectrum is determined and a unique point on the portion of the pulse height distribution spectrum representing the energy spectrum of the medium is found, a pulse height value related to such unique point providing a value related to the degree of quench present in the mixture. By comparing such pulse height value to a pulse height value obtained in a like manner for a calibration standard, a degree of quench present in the mixture is indicated. Furthermore, the counts detected for the liquid scintillation medium during a measurement period may be related to the specific activity of the liquid scintillation medium to determine the volume of liquid scintillation medium flowing during the measurement period. The volume of sample flowing during such period may also be determined by relating the liquid scintillation medium volume to a ratio of sample to liquid scintillation medium flowing through the flow detector. Sample count rate is corrected for quench and sample specific activity is determined.
Inventors
- 👤 Donald L. Horrocks
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 04:29
CPC Classifications
G; G01; G01F; G01F1/00; G01F1/704; G01F1/7042; G01T; G01T1/00; G01T1/16; G01T1/20; G01T1/204; G01T1/2042; G01T1/2045
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
219 words
A method for determining quench and volume in a liquid scintillation flow system, comprising the steps of: flowing a mixture comprising the sample in a liquid scintillation medium through a flow detector and developing a pulse height distribution spectrum for the solution, the liquid scintillation medium comprising a scintillating material and an alpha emitting radionuclide marker having a predetermined activity; detecting a unique point on the portion of the pulse height spectrum representing the energy spectrum of the marker and determining a pulse height value relating to the unique point; comparing the pulse height value of the unique point to a pulse height value obtained in a like manner for a calibration standard, the difference between such pulse height values indicating a degree of quench present in the solution; determining the number of counts of the liquid scintillation medium during the measurement period that the pulse height distribution spectrum was developed; determining the volume of the liquid scintillation medium that flowed through the photodetector during the measurement period according to: ##EQU2## where C is the number of counts detected for the liquid scintillation medium, T is the time period, L is the activity of the liquid scintillation medium in counts per unit time, and V o is the volume of liquid scintillation medium which produces the count rate L.
Claim 2
Independent Claim
219 words
A method for determining quench and volume in a liquid scintillation flow system, comprising the steps of: flowing a mixture comprising the sample in a liquid scintillation medium through a flow detector and developing a pulse height distribution spectrum for the solution, the liquid scintillation medium comprising a scintillating material and an alpha emitting radionuclide marker having a predetermined activity; detecting a unique point on the portion of the pulse height spectrum representing the energy spectrum of the marker and determining a pulse height value relating to the unique point; comparing the pulse height value of the unique point to a pulse height value obtained in a like manner for a calibration standard, the difference between such pulse height values indicating a degree of quench present in the solution; determining the number of counts of the liquid scintillation medium during the measurement period that the pulse height distribution spectrum was developed; determining the volume of the liquid scintillation medium that flowed through the photodetector during the measurement period according to: ##EQU2## where C is the number of counts detected for the liquid scintillation medium, T is the time period, L is the activity of the liquid scintillation medium in counts per unit time, and V o is the volume of liquid scintillation medium which produces the count rate L.
Claim 3
Dependent Claim
90 words
The method as in claim 1 wherein the step of flowing the sample in the liquid scintillation medium through the flow detector further includes selecting a ratio R of the volume of sample with respect to the volume of liquid scintillation medium flowing through the flow detector, and the method further includes the step of determining the volume of the sample flowing through the flow detector during the measurement time period by relating the volume of the liquid scintillation medium flowing during such measurement time period to the ratio R.
Claim 4
Dependent Claim
55 words
A method as in claim 2 further including the steps of: determining sample count rate during the measurement period; correcting sample count rate by an efficiency value related to the degree of quench present in the mixture to yield sample disintegration rate; and determining sample specific activity by dividing sample disintegration rate by sample volume.
Claim 5
Independent Claim
99 words
A method of measuring a value related to quench of a sample in a liquid scintillation flow system, comprising the steps of: flowing a mixture comprising the sample in a liquid scintillation medium through a photodetector and developing a pulse height distribution spectrum for the solution, the liquid scintillation medium comprising a scintillating material and a radionuclide marker wherein the marker has an energy distribution different from the sample; and detecting a unique point on the portion of the pulse height spectrum representing the energy of the marker and determining a pulse height value related to the unique point.
Claim 6
Independent Claim
99 words
A method of measuring a value related to quench of a sample in a liquid scintillation flow system, comprising the steps of: flowing a mixture comprising the sample in a liquid scintillation medium through a photodetector and developing a pulse height distribution spectrum for the solution, the liquid scintillation medium comprising a scintillating material and a radionuclide marker wherein the marker has an energy distribution different from the sample; and detecting a unique point on the portion of the pulse height spectrum representing the energy of the marker and determining a pulse height value related to the unique point.
Claim 7
Dependent Claim
46 words
The method of claim 4 further including comparing the pulse height value of the unique point to a pulse height value obtained in a like manner for a calibration standard, the difference between such pulse height values indicating a degree of quench present in the solution.
Claim 8
Dependent Claim
29 words
The method as in claim 4 wherein the step of detecting the unique point on the spectrum representing the energy spectrum of the marker includes determining the peak thereof.
Claim 9
Dependent Claim
24 words
A method as in claim 4 wherein the marker is selected such that the marker energy spectrum does not overlap the sample energy spectrum.
Claim 10
Dependent Claim
14 words
The method as in claim 7 wherein the marker is an alpha emitting radionuclide.
Claim 11
Independent Claim
140 words
A method of measuring quench in a liquid scintillation flow system, comprising the steps of: flowing a mixture comprising the sample in a liquid scintillation medium through a flow detector and developing a pulse height distribution spectrum for the mixture, the liquid scintillation medium comprising a scintillating material and a radionuclide marker wherein the marker is selected as having an energy spectrum that does not overlap the sample energy spectrum; detecting a unique point on the portion of the pulse height spectrum representing the energy spectrum of the marker and determining a pulse height value related to the unique point; and comparing the pulse height value of the unique point to a pulse height value obtained in a like manner for a calibration standard, the difference between such pulse height values indicating a degree of quench present in the solution.
Claim 12
Independent Claim
140 words
A method of measuring quench in a liquid scintillation flow system, comprising the steps of: flowing a mixture comprising the sample in a liquid scintillation medium through a flow detector and developing a pulse height distribution spectrum for the mixture, the liquid scintillation medium comprising a scintillating material and a radionuclide marker wherein the marker is selected as having an energy spectrum that does not overlap the sample energy spectrum; detecting a unique point on the portion of the pulse height spectrum representing the energy spectrum of the marker and determining a pulse height value related to the unique point; and comparing the pulse height value of the unique point to a pulse height value obtained in a like manner for a calibration standard, the difference between such pulse height values indicating a degree of quench present in the solution.
Claim 13
Dependent Claim
14 words
A method as in claim 9 wherein the marker is an alpha emitting radionuclide.
Claim 14
Independent Claim
178 words
A method for determining the volume of a liquid scintillation medium flowing through a liquid scintillation flow system during a predetermined time period, comprising the steps of: flowing a mixture comprising the sample in the liquid scintillation flow medium through a flow detector and developing a pulse height distribution spectrum for at least the liquid scintillation medium, the liquid scintillation medium comprising a scintillating material and a radionuclide marker wherein the marker has a predetermined activity and an energy distribution substantially different from the sample; determining the counts detected by the flow detector for the portion of the spectrum representing the energy spectrum of the liquid scintillation medium; and determining the volume of the liquid scintillation medium that flows through the flow detector during the time period according to: ##EQU3## where C is the number of counts detected for the liquid scintillation medium, T is the time period, L is the activity of the liquid scintillation medium in counts per unit time, and V o is the volume of liquid scintillation medium which produces the count rate L.
Claim 15
Independent Claim
178 words
A method for determining the volume of a liquid scintillation medium flowing through a liquid scintillation flow system during a predetermined time period, comprising the steps of: flowing a mixture comprising the sample in the liquid scintillation flow medium through a flow detector and developing a pulse height distribution spectrum for at least the liquid scintillation medium, the liquid scintillation medium comprising a scintillating material and a radionuclide marker wherein the marker has a predetermined activity and an energy distribution substantially different from the sample; determining the counts detected by the flow detector for the portion of the spectrum representing the energy spectrum of the liquid scintillation medium; and determining the volume of the liquid scintillation medium that flows through the flow detector during the time period according to: ##EQU3## where C is the number of counts detected for the liquid scintillation medium, T is the time period, L is the activity of the liquid scintillation medium in counts per unit time, and V o is the volume of liquid scintillation medium which produces the count rate L.
Claim 16
Dependent Claim
74 words
The method as in claim 11 wherein the step of flowing the mixture through the flow detector further includes selecting a ratio R of the volume of sample with respect to the volume of liquid scintillation medium flowing through the flow detector, and the method further includes the step of determining the volume of the sample flowing through the flow detector by relating the volume of the liquid scintillation medium to the ratio R.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-04-27 | 1990-10-27 | 1991-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-27 | 1994-10-27 | 1995-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-04-27 | 1998-10-27 | 1999-04-28 | $7700.00 | Window Closed / Lapsed |