US4659665A
Expired / Lapsed
US
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Enzyme membranes for electrodes
Assignee: Ramot at Tel Aviv University Ltd
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Filing: 1984-08-20
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Granted: 1987-04-21
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Expires: 2004-08-20
Abstract
Abstract A film or membrane containing a biologically active protein such as an enzyme is prepared from a polymer substituted with acyl hydrazide groups. Preferably, the polymer is an acrylamide/methacrylamide copolymer in the ratio of about 70/30, and the film or membrane is about 30-100 microns thick. The film or membrane is prepared on an electrode and crosslinked to produce an enzyme electrode.
Inventors
- 👤 Amihay Freeman
- 👤 Ruth Tor
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 11:43
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
45 words
A membrane or film comprising an acrylamide/methacrylamide copolymer in a respective molar ratio of about 70/30, substituted with acyl hydrazide groups, crosslinked with a crosslinking agent and containing a biologically active protein, said membrane or film having a thickness of about 30 to 100 microns.
Claim 2
Dependent Claim
45 words
A membrane or film comprising an acrylamide/methacrylamide copolymer in a respective molar ratio of about 70/30, substituted with acyl hydrazide groups, crosslinked with a crosslinking agent and containing a biologically active protein, said membrane or film having a thickness of about 30 to 100 microns.
Claim 3
Dependent Claim
14 words
A membrane of film according to claim 1, wherein the protein is an enzyme.
Claim 4
Dependent Claim
14 words
A membrane or film according to claim 1, wherein the cross-linking agent is glyoxal.
Claim 5
Dependent Claim
39 words
An enzyme electrode comprising a pH electrode coated with a membrane film according to claim 1 wherein the biologically active protein is an enzyme, and where the film containing the enzyme adheres to the effective surface of the electrode.
Claim 6
Dependent Claim
13 words
an enzyme electrode according to claim 4, wherein the crosslinking agent is glyoxal.
Claim 7
Dependent Claim
87 words
A process for the production of membranes or films containing a biologically active protein, adhering to solid substrates, which comprises preparing an aqueous solution of an acrylamide/methacrylamide copolymer in a respective molar ratio of about 70/30 substituted with acyl hydrazide groups and the biologically active protein, applying a film of the solution to the solid substrate to obtain a film of the copolymer having a thickness of about 30 to 100 microns, and cross-linking the copolymer by contacting said copolymer with a solution of a cross-linking agent.
Claim 8
Dependent Claim
87 words
A process for the production of membranes or films containing a biologically active protein, adhering to solid substrates, which comprises preparing an aqueous solution of an acrylamide/methacrylamide copolymer in a respective molar ratio of about 70/30 substituted with acyl hydrazide groups and the biologically active protein, applying a film of the solution to the solid substrate to obtain a film of the copolymer having a thickness of about 30 to 100 microns, and cross-linking the copolymer by contacting said copolymer with a solution of a cross-linking agent.
Claim 9
Dependent Claim
12 words
A process according to claim 6, wherein the crosslinking agent is glyoxal.
Claim 10
Dependent Claim
14 words
A process according to claim 6 wherein the solid substrate is a pH electrode.
References Cited (Backward Citations)
US3542662A
Cited
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| 3.5 Years (Year 4) | 1990-04-20 | 1990-10-20 | 1991-04-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-20 | 1994-10-20 | 1995-04-21 | $3760.00 | Window Closed / Lapsed |
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