US4657873A
Expired / Lapsed
US
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Preactivated plastics surfaces for immobilizing organo-chemical and biologic materials
Assignee: Henning Berlin GmbH Chemie- und Pharmawerk
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Filing: 1984-07-27
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Granted: 1987-04-14
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Expires: 2004-07-27
Abstract
Abstract Plastics surfaces are preactivated for immobilizing organo-chemical and biologic materials by coating the surfaces with a polypeptide which consists of a defined composition of hydrophobic amino acids and amino acids having side chains capable of activation and coupling. After activation of the plastics surfaces having been coated in this manner with various coupling reagents, it is possible to firmly fix organo-chemical and biologic materials. The materials having been immobilized in this manner are extremely stable to various reaction conditions.
Inventors
- 👤 Andre Gadow
- 👤 W. Graham Wood
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 16:22
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/543; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
78 words
A process for preactivating a hydrophobic plastic surface for the immobilization of organo-chemical and biologic materials which comprises contacting the plastic surface with a solution or suspension of a polypeptide to form a coating on the plastic surface, said polypeptide comprises a hydrophobic amino acid capable of being adsorbed by the plastic surface and an amino acid having a side chain capable of activation and coupling with said organo-chemical and biologic materials, and washing the thus coated surface.
Claim 2
Dependent Claim
78 words
A process for preactivating a hydrophobic plastic surface for the immobilization of organo-chemical and biologic materials which comprises contacting the plastic surface with a solution or suspension of a polypeptide to form a coating on the plastic surface, said polypeptide comprises a hydrophobic amino acid capable of being adsorbed by the plastic surface and an amino acid having a side chain capable of activation and coupling with said organo-chemical and biologic materials, and washing the thus coated surface.
Claim 3
Dependent Claim
16 words
A process according to claim 1, wherein the biological material is an antibody or an antigen.
Claim 4
Dependent Claim
13 words
A process according to claim 1 wherein the polypeptide is a phenylalanine-lysine copolymer.
Claim 5
Dependent Claim
54 words
A preactivated plastic surface for the immobilization of organo-chemical and biologic materials prepared by coating a hydrophobic plastic surface with a polypeptide comprising a hydrophobic amino acid capable of being adsorbed by the plastic surface and an amino acid having a side chain capable of activation and coupling with said organo-chemical and biologic materials.
Claim 6
Dependent Claim
54 words
A preactivated plastic surface for the immobilization of organo-chemical and biologic materials prepared by coating a hydrophobic plastic surface with a polypeptide comprising a hydrophobic amino acid capable of being adsorbed by the plastic surface and an amino acid having a side chain capable of activation and coupling with said organo-chemical and biologic materials.
Claim 7
Dependent Claim
18 words
A preactivated plastic surface according to claim 4, wherein the hydrophobic amino acid is phenylalanine, valine or leucine.
Claim 8
Dependent Claim
34 words
A preactivated plastic surface according to claim 4, wherein the amino acid having a side chain capable of activation and coupling is lysine, arginine, ornithine, cysteine, glutamic acid, threonine or serine or aspartic acid.
Claim 9
Dependent Claim
17 words
A preactivated plastic surface according to claim 4, wherein the surface is coated with a phenylalanine-lysine copolymer.
Claim 10
Dependent Claim
23 words
A preactivated surface according to claim 7, wherein the ratio of hydrophobic amino acid to amino acid having a side chain is 1:1.
Claim 11
Dependent Claim
17 words
A preactivated plastic surface according to claim 4, wherein the polypeptide has a molecular weight above 10,000.
References Cited (Backward Citations)
US4525465A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-04-13 | 1990-10-13 | 1991-04-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-13 | 1994-10-13 | 1995-04-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-04-13 | 1998-10-13 | 1999-04-14 | $7700.00 | Window Closed / Lapsed |