Abstract Disclosed is a method of preparing finely divided cartilage powder from raw cartilage including the steps of mechanically trimming raw cartilage from an animal body, and granulating the raw cartilage into granules having an average size of from about 4 mm to 8 mm diameter. The granules are then subjected to azeotropic extraction, the extraction conducted with an extracting agent which both forms an azeotrope with water and solubilizes fat. The granules are then separated from the extracting agent, and milled to an average size of from about 40 to 70μ diameter. Also disclosed is a method of separating calcium compounds from cartilage including the steps of mechanically trimming raw cartilage from an animal body, granulating the raw cartilage into granules having an average size of from about 4 to 8 mm diameter, and partially drying the granules to produce a friable calcified outer layer on each granule. The granules are then subjected to mechanical agitation to pulverize the friable calcified outer layer and separated from the pulverized calcified outer layer. Also disclosed is a method of preparing finely divided cartilage powder involving a freeze-drying step.
A method of preparing finely divided cartilage powder from raw cartilage comprising: isolating raw cartilage from an animal body; granulating the raw cartilage into granules having an average size of from about 4 to 8 mm diameter; freeze drying the granules until the moisture content of the granules falls below 3.0%; and milling the granules to an average size of from about 40 to 70μ mm diameter.
Claim 2
Independent Claim
67 words
A method of preparing finely divided cartilage powder from raw cartilage comprising: isolating raw cartilage from an animal body; granulating the raw cartilage into granules having an average size of from about 4 to 8 mm diameter; freeze drying the granules until the moisture content of the granules falls below 3.0%; and milling the granules to an average size of from about 40 to 70μ mm diameter.
Claim 3
Dependent Claim
21 words
The method of claim 1 further comprising treating the isolated cartilage with a proteolytic enzyme solution prior to said granulating step.
Claim 4
Dependent Claim
31 words
The method of claim 2 wherein said proteolytic enzyme solution is selected from the group consisting of pepsin, papain, trypsin, pepsin and hydrochloric acid, pepsin and acetic acid, and combintions thereof.
Claim 5
Dependent Claim
11 words
The method of claim 3 wherein said animal is a shark.