US4652599A
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US
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Method of continuous production of retroviruses (HTLV-III) from patients with AIDS and pre-AIDS using permissive cells
Assignee: United States Department of Health and Human Services
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Filing: 1984-08-24
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Granted: 1987-03-24
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Expires: 2004-08-24
Abstract
Abstract A cell system is disclosed for the reproducible detection and isolation of human T-lymphotropic retroviruses (HTLV-family) with cytopathic effects (HTLV-III) from patients with the acquired immune deficiency syndrome (AIDS), pre-AIDS and in healthy carriers. One neoplastic aneuploid T-cell line derived from an adult with lymphoid leukemia, and termed HT, was susceptible to infection with HTLV-III, which is transformed and provides T-cell populations which are highly susceptible to and permissive for HTLV-III, and convenient for large scale production, isolation, and biological detection of the virus. Other operational neoplastic T-cell lines which are positive for OKT4 marker, e.g., Molt 3, CEM, Ti7.4 and HUT78, can produce HTLV-III in a large amount and retain its unlimited capability for growth.
Inventors
- 👤 Robert C. Gallo
- 👤 Mikulas Popovic
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 12:40
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0634; C12N5/0636; C12N7/00; A; A61; A61P; A61P31/00; A61P31/12; A61P31/14; A61P31/18; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/10051; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
71 words
A method for the continuous production of HTLV-III virus which comprises infecting a T-cell line with said virus, said cell line is one member of the group selected from Molt 3, CEM, HUT78, and OKT4 + cells and said cell line(s) preserves the capacity for permanent growth after infection with said virus; growing said cell line(s) under conditions suitable for cell growth; and recovering said virus produced by said cell line(s).
Claim 2
Independent Claim
71 words
A method for the continuous production of HTLV-III virus which comprises infecting a T-cell line with said virus, said cell line is one member of the group selected from Molt 3, CEM, HUT78, and OKT4 + cells and said cell line(s) preserves the capacity for permanent growth after infection with said virus; growing said cell line(s) under conditions suitable for cell growth; and recovering said virus produced by said cell line(s).
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein said virus originates from an HTLV-III positive culture.
Claim 4
Dependent Claim
53 words
A process for the continual production of HTLV-III by infected T-cells in high producing long-term cultures which comprises cocultivating HTLV-III virus with one member of the group of cells consisting of Molt 3, CEM, and HUT78, growing said cells under conditions suitable for cell growth, and recovering said virus produced by said cells.
Claim 5
Dependent Claim
53 words
A process for the continual production of HTLV-III by infected T-cells in high producing long-term cultures which comprises cocultivating HTLV-III virus with one member of the group of cells consisting of Molt 3, CEM, and HUT78, growing said cells under conditions suitable for cell growth, and recovering said virus produced by said cells.
Claim 6
Dependent Claim
55 words
A process for producing a cell line H9/HTLV-III B which comprises infecting target HT-cells with HTLV-III virus, said infecting process overcomes the normal cytopathic effect of HTLV-III and preserves the immortal growth capacity of the target cell, and growing the infected cells under conditions suitable for cell growth and recovering said H9/HTLV-III B cell line.
Claim 7
Dependent Claim
55 words
A process for producing a cell line H9/HTLV-III B which comprises infecting target HT-cells with HTLV-III virus, said infecting process overcomes the normal cytopathic effect of HTLV-III and preserves the immortal growth capacity of the target cell, and growing the infected cells under conditions suitable for cell growth and recovering said H9/HTLV-III B cell line.
Claim 8
Dependent Claim
14 words
The method of claim 4 wherein said virus originates from an HTLV-III positive culture.
Claim 9
Independent Claim
56 words
A method for continuous production of HTLV-III virus which comprises infecting highly susceptible, permissive cells consisting of a neoplastic aneuploid T-cell line with said virus, said cells preserve the capacity for permanent growth after the infection with said virus, growing said cells under conditions suitable for cell growth, and recovering said virus produced by said cell.
Claim 10
Independent Claim
56 words
A method for continuous production of HTLV-III virus which comprises infecting highly susceptible, permissive cells consisting of a neoplastic aneuploid T-cell line with said virus, said cells preserve the capacity for permanent growth after the infection with said virus, growing said cells under conditions suitable for cell growth, and recovering said virus produced by said cell.
Claim 11
Dependent Claim
14 words
The method of claim 6 wherein said virus consists of cytopathic variants of HTLV.
Claim 12
Dependent Claim
15 words
The method of claim 6 wherein said infecting comprises cocultivating said virus with said cells.
Claim 13
Dependent Claim
17 words
The method of claim 6 wherein said infecting comprises cell-free infection of said cells with said virus.
Claim 14
Dependent Claim
19 words
The method of claim 6 wherein said cells are neoplastic aneuploid T-cells derived from an adult with lymphoid leukemia.
Claim 15
Dependent Claim
24 words
The method of claim 6 wherein said cells are one member of the group selected from Molt 3, CEM, HUT78, and OKT4 + cells.
Claim 16
Dependent Claim
14 words
The method of claim 6 wherein said virus originates from an HTLV-III positive culture.
Claim 17
Independent Claim
45 words
A method of producing a cell line which continually produces HTLV-III virus which comprises infecting a T-cell line susceptible to infection with HTLV-III, said cell line is capable of continuous large-scale production of HTLV-III, and growing said cell line under conditions suitable for cell growth.
Claim 18
Independent Claim
45 words
A method of producing a cell line which continually produces HTLV-III virus which comprises infecting a T-cell line susceptible to infection with HTLV-III, said cell line is capable of continuous large-scale production of HTLV-III, and growing said cell line under conditions suitable for cell growth.
Claim 19
Dependent Claim
27 words
The method of claim 7 wherein said cell line is one member of the group selected from Molt 3, CEM, HUT78, and a neoplastic aneuploid T-cell line.
Claim 20
Dependent Claim
20 words
The method of claim 14 wherein said HTLV-III are variants of human T-lymphotropic retrovirus, exhibit cytopathic effects and are non-transforming.
Claim 21
Dependent Claim
35 words
A cell line which continually produces HTLV-III virus consisting of one member of the group selected from Molt 3, CEM, HUT78, and OKT4 + cell lines, said cell lines are persistently infected with HTLV-III virus.
Claim 22
Dependent Claim
35 words
A cell line which continually produces HTLV-III virus consisting of one member of the group selected from Molt 3, CEM, HUT78, and OKT4 + cell lines, said cell lines are persistently infected with HTLV-III virus.
Claim 23
Dependent Claim
38 words
In an immunoligical analysis for acquired immune deficiency syndrome (AIDS) which includes the use of HTLV-III virus, the improvements which comprises employing human T-cell leukemia virus Type III infected cells of claim 16 for the detection of AIDS.
Claim 24
Dependent Claim
33 words
In an immunofluorescent assay for acquired immuno deficiency syndrome the improvement which comprises employing the human T-cell leukemia virus Type III infected cells of claim 16 for the detection of AIDS or pre-AIDS.
Claim 25
Dependent Claim
30 words
A slide for indirect immunofluorescent analysis for acquired immune deficiency syndrome comprising the human T-cell leukemia virus Type III infected cells of claim 16 dried and fixed on said slide.
References Cited (Backward Citations)
US4520113A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-03-23 | 1990-09-22 | 1991-03-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-03-23 | 1994-09-22 | 1995-03-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-03-23 | 1998-09-22 | 1999-03-24 | $7700.00 | Window Closed / Lapsed |