US4650909A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Polyethylene glycol (PEG) reagent
Assignee: Individual
•
Filing: 1985-10-29
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Granted: 1987-03-17
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Expires: 2005-10-29
Abstract
Abstract A process for production of improved polyethylene glycol (PEG) is disclosed. This process overcomes the toxicity that results from using the known (and impure) PEG-fusion reagent by incorporation of a purification method that removes the toxic elements always present in PEG preparations stored above 0° C. in the presence of oxygen.
Inventors
- 👤 George H. Yoakum
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 23:10
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/10; C12N5/12; C12N5/16; C08; C08G; C08G65/00; C08G65/02; C08G65/30; C08L; C08L71/00; C08L71/02
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
87 words
A process for the production of polyethylene glycol (PEG) molecular weight, 1000-6000 for human cell genetic transfection comprising melting PEG; adding a resin to said PEG to form a PEG/resin slurry at a pH of about 7.4, said resin consisting of cationic and anionic resins selected from the group consisting of a mixed bed resin containing a styrene-divinyl benzene backbone, a cross-linked dextran containing a diethyl (2-hydroxypropyl)amino ethyl functional group, and mixtures thereof; filtering the PEG/resin slurry through fresh mixed bed resin to obtain the improved PEG.
Claim 2
Dependent Claim
87 words
A process for the production of polyethylene glycol (PEG) molecular weight, 1000-6000 for human cell genetic transfection comprising melting PEG; adding a resin to said PEG to form a PEG/resin slurry at a pH of about 7.4, said resin consisting of cationic and anionic resins selected from the group consisting of a mixed bed resin containing a styrene-divinyl benzene backbone, a cross-linked dextran containing a diethyl (2-hydroxypropyl)amino ethyl functional group, and mixtures thereof; filtering the PEG/resin slurry through fresh mixed bed resin to obtain the improved PEG.
Claim 3
Dependent Claim
25 words
The process of claim 1 wherein the resin is said mixed bed divinylbenzene resin in combination with said dextran resin with an amino functional group.
Claim 4
Dependent Claim
21 words
The process in claim 1 wherein said improved PEG is 1000 molecular weight and a melting point of about 42° C.
Claim 5
Dependent Claim
87 words
A fusion grade reagent suitable for use in human cell fusion produced by the process comprising melting polyethylene glycol (PEG) molecular weight, 1000-6000 adding a resin to form a PEG/resin slurry at a pH of about 7.4, said resin consisting of cationic and anionic resins selected from the group consisting of a mixed bed resin containing a styrene-divinyl benzene backbone, a cross-linked dextran containing a diethyl (2-hydroxypropyl)-amino ethyl functional group, and mixtures thereof; filtering the PEG/resin slurry through fresh mixed bed resin to obtain the improved PEG.
Claim 6
Dependent Claim
87 words
A fusion grade reagent suitable for use in human cell fusion produced by the process comprising melting polyethylene glycol (PEG) molecular weight, 1000-6000 adding a resin to form a PEG/resin slurry at a pH of about 7.4, said resin consisting of cationic and anionic resins selected from the group consisting of a mixed bed resin containing a styrene-divinyl benzene backbone, a cross-linked dextran containing a diethyl (2-hydroxypropyl)-amino ethyl functional group, and mixtures thereof; filtering the PEG/resin slurry through fresh mixed bed resin to obtain the improved PEG.
Claim 7
Dependent Claim
27 words
The fusion grade reagent in claim 4 wherein said resin is said mixed bed divinylbenzene resin in combination with said dextran resin with an amino functional group.
Claim 8
Dependent Claim
24 words
The reagent of claim 4 wherein said fusion grade reagent is 1000 molecular weight polyethylene glycol with a melting point of about 42° C.
References Cited (Backward Citations)
US2812360A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-03-16 | 1990-09-15 | 1991-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-03-16 | 1994-09-15 | 1995-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-03-16 | 1998-09-15 | 1999-03-17 | $7700.00 | Window Closed / Lapsed |