US4650753A
Expired / Lapsed
US
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Novel substrates for use in measuring the concentration of kallikrein in urine
Assignee: Nitto Boseki Co Ltd
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Filing: 1985-06-27
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Granted: 1987-03-17
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Expires: 2005-06-27
Abstract
Abstract A novel chromogenic and fluorescent substrate for measuring the concentration of kallikrein in urine. The novel substrate according to the present invention has very excellent substrate specificity to kallikreins and good solubility in water or biological test solutions. The present substrate is therefore useful for measuring the concentration of kallikrein in urine for the diagnosis of hypertension and other diseases caused by the lowering of the concentration of kallikrein.
Inventors
- 👤 Takeshi Nagasawa
- 👤 Yoshio Nakamura
- 👤 Katsumasa Kuroiwa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 13:05
CPC Classifications
C; C07; C07K; C07K5/00; C07K5/04; C07K5/08; C07K5/0821; C07K5/0802; C07K5/0804; C07K5/0806; C07K5/0808; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C12Q2334/00; C12Q2334/10; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/95; G01N2333/964; G01N2333/96425; G01N2333/96427; G01N2333/9643; G01N2333/96433; G01N2333/96441; G01N2333/96455; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S530/00; Y10S530/802
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
39 words
A composition of matter having the general formula (I): ##STR3## wherein A 1 is Pro (prolyl group), PGlu (pyroglutamyl group), Val (valyl group), Ala (alanyl group), Leu (leucyl group), Phe (phenylalanyl group) or Lys (lysyl group) or salt thereof.
Claim 2
Dependent Claim
39 words
A composition of matter having the general formula (I): ##STR3## wherein A 1 is Pro (prolyl group), PGlu (pyroglutamyl group), Val (valyl group), Ala (alanyl group), Leu (leucyl group), Phe (phenylalanyl group) or Lys (lysyl group) or salt thereof.
Claim 3
Dependent Claim
14 words
The composition of matter of claim 1 wherein A 1 is a valyl group.
Claim 4
Dependent Claim
14 words
The composition of matter of claim 1 wherein A 1 is an alanyl group.
Claim 5
Independent Claim
80 words
A method of determining the activity of kallikrein in the urine which comprises reacting a compound having the general formula: ##STR4## wherein A 1 is Pro (prolyl group), PGlu (pyroglutamyl group), Val (valyl group), Ala (alanyl group), Leu (leucyl group), Phe (phenylalanyl group) or Lys (lysyl group) or salt thereof with urine to form 3-carboxy-4-hydroxy aniline; transforming the 3-carboxy-4-hydroxy aniline into a colored substance; and determining the activity of the kallikrein by colorimetric or fluorometric analysis of the 3-carboxy-4-hydroxy aniline.
Claim 6
Independent Claim
80 words
A method of determining the activity of kallikrein in the urine which comprises reacting a compound having the general formula: ##STR4## wherein A 1 is Pro (prolyl group), PGlu (pyroglutamyl group), Val (valyl group), Ala (alanyl group), Leu (leucyl group), Phe (phenylalanyl group) or Lys (lysyl group) or salt thereof with urine to form 3-carboxy-4-hydroxy aniline; transforming the 3-carboxy-4-hydroxy aniline into a colored substance; and determining the activity of the kallikrein by colorimetric or fluorometric analysis of the 3-carboxy-4-hydroxy aniline.
Claim 7
Dependent Claim
12 words
The method of claim 4 wherein A 1 is a valyl group.
Claim 8
Dependent Claim
12 words
The method of claim 4 wherein A 1 is an alanyl group.
References Cited (Backward Citations)
US3884896A
Cited
US3886136A
Cited
US4137225A
Cited
US4279810A
Cited
US4327178A
Cited
US4457866A
Cited
US4452736A
Cited
US4448715A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-03-16 | 1990-09-15 | 1991-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-03-16 | 1994-09-15 | 1995-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-03-16 | 1998-09-15 | 1999-03-17 | $7700.00 | Window Closed / Lapsed |