US4647543A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for analyses to be carried out on immobilized biological tissue
Assignee: Individual
•
Filing: 1984-02-24
•
Granted: 1987-03-03
•
Expires: 2004-02-24
Abstract
Abstract Processes for testing immobilized biological material with generally biochemical and histochemical methods, particularly enzyme, immuno and hormone chemical methods can be improved by adhering the biological material to the surface of a support and then fixing the support to a plate. The plate is constructed in such a way that the biological material is protected from damage. Using a biological testing technique such as thin section immunofluorescence testing the invention makes it possible to carry out biochemical tests in a more rational and trouble-free manner than with hitherto known methods. If necessary, a random number of tests can be performed side-by-side on a single plate. The invention makes it possible to simply and rapidly prepare frozen section products and store them in a space-saving manner at very low temperatures. The adhesion of the frozen sections to the support can be improved by coupling chemicals which react with the tissue by bonding thereto to the support.
Inventors
- 👤 Winfried Stocker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 02:26
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/551; G01N33/552; G01N1/00; G01N1/28; G01N1/286; G01N1/42; G01N2001/2873; G01N2474/00; G01N2474/20; Y; Y10; Y10T; Y10T436/00; Y10T436/25
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
68 words
A process for preparing biological tissue, for storage and testing thereof comprising the steps of: (1) immobilizing a biological tissue by adhering it to a surface of at least one dividable support having at least two surfaces; (2) breaking or cutting said dividable support into at least one smaller support; and (3) fixing said at least one smaller support with the immobilized biological tissue thereon onto a plate.
Claim 2
Dependent Claim
68 words
A process for preparing biological tissue, for storage and testing thereof comprising the steps of: (1) immobilizing a biological tissue by adhering it to a surface of at least one dividable support having at least two surfaces; (2) breaking or cutting said dividable support into at least one smaller support; and (3) fixing said at least one smaller support with the immobilized biological tissue thereon onto a plate.
Claim 3
Dependent Claim
30 words
The process of claim 1 wherein said plate has at least one depression therein and said immobilized biological tissue is fixed to reaction fields in said at least one depression.
Claim 4
Dependent Claim
14 words
The process of claim 1 wherein said biological tissue is a thin histological section.
Claim 5
Dependent Claim
16 words
The process of claim 1 wherein said at least one dividable support is a glass disc.
Claim 6
Dependent Claim
54 words
The process of claim 1 wherein said biological tissue comprises frozen sections which are adhered to the surface of said support by: coupling to said support a chemical substance having groups reactive with said frozen sections, and chemically reacting said frozen sections with said reactive groups, thereby covalently bonding said sections to said support.
Claim 7
Dependent Claim
96 words
A process for preparing biological tissue for storage and testing thereof comprising the steps of: (1) immobilizing a biological tissue by adhering it to a surface of at least one dividable support having at least two surfaces; (2) breaking or cutting said biological support into at least one smaller support; and (3) fixing said at least one smaller support with the immobilized biological tissue thereon within at least one depression in a plate, said at least one depression having a depth equal to or greater than the combined thickness or said support and said biological material.
Claim 8
Dependent Claim
96 words
A process for preparing biological tissue for storage and testing thereof comprising the steps of: (1) immobilizing a biological tissue by adhering it to a surface of at least one dividable support having at least two surfaces; (2) breaking or cutting said biological support into at least one smaller support; and (3) fixing said at least one smaller support with the immobilized biological tissue thereon within at least one depression in a plate, said at least one depression having a depth equal to or greater than the combined thickness or said support and said biological material.
Claim 9
Dependent Claim
26 words
The process of claim 6 wherein said at least one depression is covered with a cover slide which is supported by a surface of said plate.
Claim 10
Dependent Claim
61 words
The process of claim 6 wherein said at least one depression defines a base of a flow cell, said at least one support having biological tissue immobilized thereon being fixed to said base at a distance Δh≧0 from a top surface of said cell, and wherein reagent solutions used for testing said biological tissue are caused to flow through said cell.
Claim 11
Dependent Claim
54 words
The process of claim 6 wherein said biological tissue comprises frozen sections which are adhered to the surface of said support by: coupling to said support a chemical substance having groups reactive with said frozen sections, and chemically reacting said frozen sections with said reactive groups, thereby covalently bonding said sections to said support.
Claim 12
Dependent Claim
56 words
A process for adhering thin sections of frozen biological tissue to a support, comprising the steps of: coupling to a support a chemical substance having reactive groups which react with a frozen biological tissue; and chemically reacting said frozen biological tissue with said reactive groups and thereby covalently bonding said frozen biological tissue to said support.
Claim 13
Dependent Claim
56 words
A process for adhering thin sections of frozen biological tissue to a support, comprising the steps of: coupling to a support a chemical substance having reactive groups which react with a frozen biological tissue; and chemically reacting said frozen biological tissue with said reactive groups and thereby covalently bonding said frozen biological tissue to said support.
Claim 14
Dependent Claim
45 words
The process of claim 10, wherein: said support is made of a material which reacts with silanes, and said chemical substance is a silane having at least one amino group which has been reacted with at least one compound having at least two aldehyde groups.
Claim 15
Dependent Claim
61 words
The process of claim 10, wherein: said support is made of a material which reacts with silanes, and said chemical substance is a silane having at least one methacryl group or acryl group which can be reacted with at least one compound having at least one group which reacts with methacryl groups or acryl groups and at least one aldehyde group.
Claim 16
Dependent Claim
30 words
The process of claim 10, wherein: said support is made of a material which reacts with silanes, and said chemical substance is a silane having at least one mercapto group.
References Cited (Backward Citations)
US2965219A
Cited
DE1133598B
Cited
US3556633A
Cited
US3720470A
Cited
US4003707A
Cited
US4353856A
Cited
US4339241A
Cited
US4387164A
Cited
US4404181A
Cited
US4435508A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-03-02 | 1990-09-01 | 1991-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-03-02 | 1994-09-01 | 1995-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-03-02 | 1998-09-01 | 1999-03-03 | $7700.00 | Window Closed / Lapsed |