US4645829A
Expired / Lapsed
US
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Method for separating polypeptides
Assignee: Monsanto Technology LLC
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Filing: 1984-10-29
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Granted: 1987-02-24
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Expires: 2004-10-29
Abstract
Abstract A method of separating a first polypeptide fraction from a second polypeptide fraction of a mixed solution is disclosed. A predetermined charged polymer is admixed with the mixed solution preferably in the presence of a neutral polymer. The charged polymer interacts with the first fraction to form a precipitate. The neutral polymer enhances the effectiveness of the charged polymer in precipitating the first fraction. By proper selection of the polymer combination the desired polypeptide fraction can be precipitated from solution leaving the other polypeptide fraction in solution or vice versa.
Inventors
- 👤 Sa Van Ho
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 11:02
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/14; C07K1/30; C07K1/32; Y; Y10; Y10S; Y10S210/00; Y10S210/902; Y10S210/903; Y10S210/905
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
83 words
A method of separating a first polypeptide fraction from a second polypeptide fraction of a mixed solution containing said fractions which comprises admixing at least one neutral polymer and at least one charged polymer with said solution, said charged polymer being soluble in said mixed solution and interacting with said first fraction to form an interaction product which precipitates from solution, said neutral polymer being soluble in said mixed solution and enhancing the effectiveness of said charged polymer in precipitating said first fraction.
Claim 2
Independent Claim
83 words
A method of separating a first polypeptide fraction from a second polypeptide fraction of a mixed solution containing said fractions which comprises admixing at least one neutral polymer and at least one charged polymer with said solution, said charged polymer being soluble in said mixed solution and interacting with said first fraction to form an interaction product which precipitates from solution, said neutral polymer being soluble in said mixed solution and enhancing the effectiveness of said charged polymer in precipitating said first fraction.
Claim 3
Dependent Claim
17 words
A method of claim 1 in which the mixed solution comprising the polypeptides is an aqueous solution.
Claim 4
Dependent Claim
22 words
A method of claim 2 in which the charged polymer is admixed with the solution in the presence of the neutral polymer.
Claim 5
Dependent Claim
27 words
A method of claim 2 in which the effectiveness of the charged polymer is enhanced by lowering the concentration of the charged polymer required to cause precipitation.
Claim 6
Dependent Claim
28 words
A method of claim 2 in which the effectiveness of the charged polymer is enhanced by increasing the selectivity of the charged polymer for precipitating said first fraction.
Claim 7
Dependent Claim
14 words
A method of claim 2 in which the mixed solution comprises a cell extract.
Claim 8
Dependent Claim
14 words
A method of claim 2 in which the mixed solution is a fermentation broth.
Claim 9
Dependent Claim
16 words
A method of claim 2 in which a biologically active polypeptide is separated from contaminating polypeptides.
Claim 10
Dependent Claim
15 words
A method of claim 2 in which a mammalian hormone is separated from contaminating polypeptides.
Claim 11
Dependent Claim
15 words
A method of claim 2 in which an avian hormone is separated from contaminating polypeptides.
Claim 12
Dependent Claim
14 words
A method of claim 2 in which a somatotropin is separated from contaminating polypeptides.
Claim 13
Dependent Claim
46 words
A method of claim 2 in which the charged and neutral polymers are chosen such that the neutral polymer is substantially similar in hydrophobic/hydrophilic character to said second fraction or substantially dissimilar in hydrophobic/hydrophilic character to the interaction product of said charged polymer and first fraction.
Claim 14
Dependent Claim
34 words
A method of claim 2 in which said second fraction is more hydrophobic than said interaction product and the neutral polymer is more like said second fraction than said interaction product in hydrophobic/hydrophilic character.
Claim 15
Dependent Claim
35 words
A method of claim 2 in which said interaction product is more hydrophobic than said second fraction and the neutral polymer is more like said second fraction than said interaction product in hydrophobic hydrophilic character.
Claim 16
Dependent Claim
14 words
A method of claim 2 in which the polypeptide fractions consist essentially of proteins.
Claim 17
Independent Claim
75 words
A method of separating a bovine somatotropin fraction from a contaminating polypeptide fraction of a mixed solution which comprises admixing at least one neutral polymer and at least one charged polymer with said solution, said charged polymer being soluble in said mixed solution and interacting with one of the fractions thereby precipitating said fraction, said neutral polymer being soluble in said mixed solution and enhancing the effectiveness of said charged polymer in precipitating said fraction.
Claim 18
Independent Claim
75 words
A method of separating a bovine somatotropin fraction from a contaminating polypeptide fraction of a mixed solution which comprises admixing at least one neutral polymer and at least one charged polymer with said solution, said charged polymer being soluble in said mixed solution and interacting with one of the fractions thereby precipitating said fraction, said neutral polymer being soluble in said mixed solution and enhancing the effectiveness of said charged polymer in precipitating said fraction.
Claim 19
Dependent Claim
19 words
A method of claim 16 in which the mixed solution comprising the bovine sometotropin fraction is an aqueous solution.
Claim 20
Dependent Claim
31 words
A method of claim 17 in which the contaiminating proteins are precipitated by the addition of diethylaminoethyldextran and the effectiveness of said diethylaminoethyldextran is enhanced by the addition of polyethylene glycol.
Claim 21
Dependent Claim
31 words
A method of claim 17 in which the contaminating proteins are precipitated by the addition of diethylaminoethyldextran and the effectiveness of said diethylaminoethyldextran is enhaneed by the addition of polypropylene glycol.
Claim 22
Dependent Claim
30 words
A method of claim 17 in which the contaminating proteins are precipitated by the addition of diethylaminoethyldextran and the effectiveness of said diethylaminoethyldextran is enhanced by the addition of polyvinylalcohol.
Claim 23
Dependent Claim
31 words
A method of claim 17 in which the contaminating proteins are precipitated by the addition of polyehtyleneimine and the effectiveness of said polyethyleneimine is enhanced by the addition of polyethylene glycol.
Claim 24
Dependent Claim
34 words
A method of claim 17 in which the contaminating proteins are precipitated by the addition of a cationic polyamine and the effectiveness of said cationic polyamine is enhanced by the addition of polyethylene glycol.
Claim 25
Dependent Claim
38 words
A method of claim 17 in which N-methionyl bovine somatotropin is separated by the precipitation of the contaminating proteins by the addition of diethylaminoethyldextran and the effectiveness of said diethylainoethyldextran is enhanced by the addition of polyethylene glycol.
Claim 26
Dependent Claim
36 words
A method of claim 17 in which N-methionyl bovine somatotropin is separated from contaminating E. coli proteins by the addition of diethylaminoethyldextran and the effectiveness of said diethylaminoethyldextran is enhanced by the additional of polyethylene glycol.
Claim 27
Dependent Claim
20 words
A method of claim 24 in which the separation is conducted at a solution pH between about 9 and 12.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-23 | 1990-08-25 | 1991-02-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-23 | 1994-08-25 | 1995-02-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-23 | 1998-08-25 | 1999-02-24 | $7700.00 | Window Closed / Lapsed |