US4645739A
Expired / Lapsed
US
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Process and reagent for the determination of N-carbamoylsarcosine with the use of a new enzyme
Assignee: Roche Diagnostics GmbH
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Filing: 1983-12-16
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Granted: 1987-02-24
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Expires: 2003-12-16
Abstract
Abstract The present invention provides a process for the determination of N-carbamoylsarcosine, wherein a sample solution containing N-carbamoylsarcosine is reacted with N-carbamoylsarcosine-amidohydrolase to give sarcosine, which is then determined. The present invention also provides the enzyme N-carbamoylsarcosine-amidohydrolase, a process for obtaining it and a reagent containing it.
Inventors
- 👤 Rolf Deeg
- 👤 Albert Roder
- 👤 Joachim Siedel
- 👤 Helmgard Gauhl
- 👤 Joachim Ziegenhorn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 05:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/70; C; C12; C12N; C12N9/00; C12N9/14; C12N9/78; C12N9/80; C12Q; C12Q1/00; C12Q1/34; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/978; G01N2333/98; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/814; Y10S435/815; Y10S435/816; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/859
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
34 words
A process for the determination of N-carbamoylsarcosine in a sample, comprising reacting said sample with N-carbamoylsarcosine-amidohydrolase to give sarcosine; and determining the sarcosine formed as an indication that N-carbamoylsarcosine is present in the sample.
Claim 2
Dependent Claim
34 words
A process for the determination of N-carbamoylsarcosine in a sample, comprising reacting said sample with N-carbamoylsarcosine-amidohydrolase to give sarcosine; and determining the sarcosine formed as an indication that N-carbamoylsarcosine is present in the sample.
Claim 3
Dependent Claim
26 words
Process according to claim 1, wherein sarcosine is determined by reacting said sarcosine with oxygen in the presence of sarcosine oxidase and determining consumption of oxygen.
Claim 4
Dependent Claim
23 words
Process according to claim 2 wherein the reaction is carried out in buffered solution at a pH value of from 6 to 9.
Claim 5
Dependent Claim
23 words
Process according to claim 1, wherein the reaction is carried out in buffered solution at a pH value of from 6 to 9.
Claim 6
Dependent Claim
38 words
Process according to claim 1, wherein the amount of sarcosine is determined by reacting sarcosine with oxygen in the presence of sarcosine oxidase to form hydrogen peroxide, and determining the hydrogen peroxide as a measure of the sarcosine.
Claim 7
Dependent Claim
15 words
Substantially pure enzyme N-carbamoylsarcosine-amidohydrolase which catalyzes formation of sarcosine, carbon dioxide and ammonia from N-carbamoylsarcosine.
Claim 8
Dependent Claim
15 words
Substantially pure enzyme N-carbamoylsarcosine-amidohydrolase which catalyzes formation of sarcosine, carbon dioxide and ammonia from N-carbamoylsarcosine.
Claim 9
Dependent Claim
25 words
A process for obtaining an enzyme, N-carbamoylsarcosine-amidohydrolase, comprising culturing micro-organisms containing said enzyme on a medium containing N-methylhydantoin and separating said N-carbamoylsarcosine-amidohydrolase from the culture.
Claim 10
Dependent Claim
25 words
A process for obtaining an enzyme, N-carbamoylsarcosine-amidohydrolase, comprising culturing micro-organisms containing said enzyme on a medium containing N-methylhydantoin and separating said N-carbamoylsarcosine-amidohydrolase from the culture.
Claim 11
Dependent Claim
18 words
Process according to claim 7 wherein there is used a micro-organisms of the genus Arthrobacter, Micrococcus or Moraxella.
Claim 12
Dependent Claim
22 words
Process according to claim 8, wherein the is used Arthrobacter DSM 2563, DSM 2564, Micrococcus spec. DSM 2565 or Moraxella DSM 2562.
Claim 13
Dependent Claim
35 words
Reagent for the determination of N-carbamoyl sarcosine comprising a mixture of N-carbamoylsarcosine-amidohydrolase, sarcosine oxidase, a compatible system for the detection of hydrogen peroxide, and a compatible buffer of from about pH 6 to about 9.
Claim 14
Dependent Claim
35 words
Reagent for the determination of N-carbamoyl sarcosine comprising a mixture of N-carbamoylsarcosine-amidohydrolase, sarcosine oxidase, a compatible system for the detection of hydrogen peroxide, and a compatible buffer of from about pH 6 to about 9.
Claim 15
Dependent Claim
10 words
Reagent of claim 10, further comprising a compatible surface-active agent.
Claim 16
Dependent Claim
33 words
A kit for determination of N-carbamoyl sarcosine comprising individually packaged N-carbamoylsarcosine-amidohydrolase, sarcosine oxidase, a compatible system for detection of hydrogen peroxide, and a buffering agent of from about pH 6 to about 9.
Claim 17
Dependent Claim
33 words
A kit for determination of N-carbamoyl sarcosine comprising individually packaged N-carbamoylsarcosine-amidohydrolase, sarcosine oxidase, a compatible system for detection of hydrogen peroxide, and a buffering agent of from about pH 6 to about 9.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-23 | 1990-08-25 | 1991-02-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-23 | 1994-08-25 | 1995-02-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-23 | 1998-08-25 | 1999-02-24 | $7700.00 | Window Closed / Lapsed |