Patent US1676360A successfully refreshed from Google Patents.
US4645618A
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Water soluble floors
Assignee: Revvity Health Sciences Inc
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Filing: 1985-11-01
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Granted: 1987-02-24
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Expires: 2005-11-01
Abstract
Abstract Water soluble fluors useful in enhancing images obtained in autoradiography have the general formula: [F].sub.x [B].sub.y [S].sub.z F is a moiety which acts as a fluor, e.g., a dehydro derivative of a compound which is a fluor; preferred examples include 4-(2,5-diphenyloxazolyl), 1-napththylmethyl, 2-napththyl, and 4-[5-(2-phenyloxazolyl)]phenyl. S is a surfactant moiety which makes the molecule hydrophilic, e.g., dehydro derivatives of surfactants; preferred examples include polyethylene glycol derivatives, sulfonic acids or salts thereof, aryl sulfonates amines and quaternary ammonium compounds. B is a binding moiety which chemically binds the fluor moiety F with the surfactant S, and may be a chemical bond, or a disubstituted moiety such as an alkylene, e.g., methylene group. The variables x, y and z are integers which can generally vary from 1 to 10. A number of such compounds are disclosed, as well as methods of using such compounds, as well as compositions and products for fluorographic analysis in connection with chromotography, electrophoresis etc.
Inventors
- 👤 Duk H. Lee
- 👤 Susan Feierberg
- 👤 Robert E. O'Brien
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 11:44
CPC Classifications
G; G01; G01T; G01T1/00; G01T1/16; G01T1/20; G01T1/204; G01T1/2042; G01T1/29; G01T1/2914; G01T1/2921; G01T1/2942
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
101 words
A method of enhancing the production of autoradiographic images by radioactive emitters contained in an absorbent or adsorbent separation medium, comprising contacting the separation medium with a fluorographic compositions, which composition comprises at least two water soluble fluors, each fluor having a structure in accordance with the formula: [F].sub.x [B].sub.y [S].sub.z wherein F is a component which absorbs energy and emits electromagnetic energy, S is a component which makes the fluor water soluble, and B is a chemical bond or other component which bonds a component F with a component S, and x, y and z are from 1 to 10.
Claim 2
Independent Claim
101 words
A method of enhancing the production of autoradiographic images by radioactive emitters contained in an absorbent or adsorbent separation medium, comprising contacting the separation medium with a fluorographic compositions, which composition comprises at least two water soluble fluors, each fluor having a structure in accordance with the formula: [F].sub.x [B].sub.y [S].sub.z wherein F is a component which absorbs energy and emits electromagnetic energy, S is a component which makes the fluor water soluble, and B is a chemical bond or other component which bonds a component F with a component S, and x, y and z are from 1 to 10.
Claim 3
Dependent Claim
23 words
The method of claim 1, wherein the fluorographic composition contains at least one water soluble fluor having a quantum efficiency above about 0.6.
Claim 4
Dependent Claim
25 words
The method of claim 1, wherein the fluorographic composition is mixed with the separation medium prior to inclusion of the radioactive emitters in said medium.
Claim 5
Dependent Claim
50 words
The method of claim 1, wherein the radioactive emitters are contained in the separation medium prior to contact with the fluorographic composition, and the fluorographic composition is contacted with the separation medium in order to permit water soluble fluor contained in said fluorographic composition to transfer to the separation medium.
Claim 6
Dependent Claim
28 words
The method of claim 3 or 4, further comprising drying the separation medium containing the water soluble fluors, and exposing a photosensitive film to the dried separation medium.
Claim 7
Independent Claim
123 words
A method of enhancing the production of autoradiographic images from radioactive emitters containing an absorbent or adsorbent separation medium, comprising contacting the separation medium with a fluorographic composition, which composition comprises at least one water soluble fluor having a structure in accordance with the following formula: [F].sub.x [B].sub.y [S].sub.z wherein F is an organic radical which absorbs energy and emits electromagnetic energy, derived from 2,5-diphenyloxazole, 2,5-diphenyl-4-methyloxazole, naphthalene, 1-methyl napthalene, m-terphenyl, p-terphenyl, 3,3'-dimethyl-p-terphenyl, fluorene, 1,2-benzofluorene, 1-methyl fluorene, isopropyl phenyl biphenylyloxadiozole, 2-[1-napthyl]-5-phenyloxazole, t-butyl phenyl biphenylyl oxadiozole, S is a surfactant radical which renders the fluor water soluble, B is a chemical bond or other component which bonds a component F with a component S, and x, y and z are integers from 1 to 10.
Claim 8
Independent Claim
123 words
A method of enhancing the production of autoradiographic images from radioactive emitters containing an absorbent or adsorbent separation medium, comprising contacting the separation medium with a fluorographic composition, which composition comprises at least one water soluble fluor having a structure in accordance with the following formula: [F].sub.x [B].sub.y [S].sub.z wherein F is an organic radical which absorbs energy and emits electromagnetic energy, derived from 2,5-diphenyloxazole, 2,5-diphenyl-4-methyloxazole, naphthalene, 1-methyl napthalene, m-terphenyl, p-terphenyl, 3,3'-dimethyl-p-terphenyl, fluorene, 1,2-benzofluorene, 1-methyl fluorene, isopropyl phenyl biphenylyloxadiozole, 2-[1-napthyl]-5-phenyloxazole, t-butyl phenyl biphenylyl oxadiozole, S is a surfactant radical which renders the fluor water soluble, B is a chemical bond or other component which bonds a component F with a component S, and x, y and z are integers from 1 to 10.
Claim 9
Dependent Claim
45 words
The method of claim 6, wherein S is a surfactant radical derived from a polyethylene glycol, or a water soluble salt thereof, a primary, secondary or tertiary amine, a quaternary ammonium salt, an amide or an imide, or a sulfonic acid radical or salt thereof.
Claim 10
Dependent Claim
47 words
The method of claim 7, wherein S is a surfactant radical derived from a polyethylene glycol having a molecular weight of about 200 to about 1500, an aryl sulfonate, an amine, an amide, a quaternary ammonium salt, or S is a sulfonic acid radical or salt thereof.
Claim 11
Dependent Claim
21 words
The method of claim 6, wherein B is a chemical bond or an alkylene, alkenyl, carboxy, arylene, alkarylene, or thio group.
Claim 12
Dependent Claim
20 words
The method of claim 9, wherein B is a chemical bond or an alkylene group having from 1-6 carbon atoms.
Claim 13
Dependent Claim
26 words
The method of claim 6, wherein the fluorographic composition is mixed with the separation medium prior to the inclusion of the radioactive emitters in said medium.
Claim 14
Dependent Claim
38 words
The method of claim 11, wherein the separation medium after admixture contains from about 0.01% to 85% by weight of water soluble fluor, based on the weight of the solids component of the separation medium prior to admixture.
Claim 15
Dependent Claim
52 words
The method of claim 6, wherein the radioactive emitters are contained in the separation medium prior to contact with the fluorographic composition, and the fluorographic composition is contacted with the separation medium in order to permit at least one water soluble fluor to transfer from the fluorographic composition into the separation medium.
Claim 16
Dependent Claim
20 words
The method of claim 13, wherein the contact is accomplished by immersion of the separation medium in the fluorographic compositions.
Claim 17
Dependent Claim
23 words
The method of claim 14, wherein the separation material is contacted with the fluorographic composition for a period of less than two hours.
Claim 18
Dependent Claim
23 words
The method of claim 14, wherein the fluorographic composition contains water soluble fluor in an amount of from about 0.001M to about 2M.
Claim 19
Dependent Claim
22 words
The method of claim 13, wherein the fluorographic composition contains water soluble fluor in an amount of about 0.005M to about 1.0M.
Claim 20
Dependent Claim
21 words
The method of claim 13, wherein the fluorographic composition contains water soluble fluor in an amount of about 0.15M to 0.4M.
Claim 21
Dependent Claim
17 words
The method of claim 13, wherein the fluorographic composition contains the water soluble fluor methoxypolyethyleneglycol 4-(2,5-diphenyloxazolyl)methyl ether.
Claim 22
Dependent Claim
42 words
The method of claim 13, wherein the fluorographic composition contains 4-[5-(2-phenyloxazolyl)]benzene sulfonic acid or a soluble salt thereof in a concentration of about 0.005M to 1M and napthalene-2-sulfonic acid or a soluble salt thereof in a concentration of about 0.005 to 1M.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-02-23 | 1990-08-25 | 1991-02-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-23 | 1994-08-25 | 1995-02-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-23 | 1998-08-25 | 1999-02-24 | $7700.00 | Window Closed / Lapsed |