US4642118A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Man-made skin composed of two layers: collagen and a poly-alpha-amino acid
Assignee: Koken Co Ltd
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Filing: 1985-06-06
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Granted: 1987-02-10
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Expires: 2005-06-06
Abstract
Abstract This invention concerns a double-layered man-made skin which is prepared by laminating a collagen sponge sheet and a poly-α-amino acid membrane that has a good affinity with tissue cells and an appropriate permeability for moisture. When the man-made skin is applied on burns, cuts or wounds, the fibroblasts proliferate in the collagen sponge sheet forming a three-dimensional structure, while the epidermal cells proliferate in the region between the poly-α-amino acid membrane and a collagen sponge sheet. The poly-α-amino acid membrane plays a role in protecting affected part and in providing an optimum condition for the proliferation of fibroblasts and epidermal cells, and then it falls off as the epidermis completely regenerates. On the other hand, the collagen sponge sheet assimilates in the living tissue after having played a general role of the dermis.
Inventors
- 👤 Yoshimitsu Kuroyanagi
- 👤 Teruo Miyata
- 👤 Manabu Seno
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:53
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/50; A61L27/60; A61L27/28; A61L27/34
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
44 words
A man-made skin composed of two layers: collagen and a poly-α-amino acid, which is characterized by comprising sticking on a membrane of collagen sponge a membrane of a poly-α-amino acid which has a good affinity with tissue cells and an appropriate permeability for moisture.
Claim 2
Dependent Claim
44 words
A man-made skin composed of two layers: collagen and a poly-α-amino acid, which is characterized by comprising sticking on a membrane of collagen sponge a membrane of a poly-α-amino acid which has a good affinity with tissue cells and an appropriate permeability for moisture.
Claim 3
Dependent Claim
27 words
A man-made skin as set forth in claim 1, in which said poly-α-amino acid is a copolymer which has a hydrophilic moiety and a hydrophobic moiety together.
Claim 4
Dependent Claim
38 words
A man-made skin as set forth in claim 2 in which the poly-α-amino acid is a copoly-(γ-benzyl-L-glutamyl-N 5 -hydroxyalkyl-L-glutamine) of the formula ##STR5## where m=2 to 4 and x represents the molar ratios of the glutamine α-amino acids.
Claim 5
Dependent Claim
34 words
A man-made skin as set forth in claim 2 in which the poly-α-amino acid is a copoly(γ-benzyl-L-glutamyl-N 5 -dihydroxyethylaminopropyl-L-glutamine) of the formula ##STR6## where x represents the molar ratios of the glutamine α-amino acids.
Claim 6
Dependent Claim
28 words
The man-made skin as set forth in claim 2 wherein the poly-α-amino acid copolymer has a molecular weight in the range of from abotu 100,000 to about 200,000.
Claim 7
Dependent Claim
176 words
A composite two-layer membrane suitable as an artificial skin for the promotion of healing of burns, cuts or wounds, which comprises a collagen sponge base layer to be used in contact with the exposed endothelium at the site of the burn, cut or wound, said collagen sponge having an apparent density of from 0.01 to 0.05 g/cm 3 , a thickness in the range of from about 0.2 to about 0.3 mm, and a pore structure such that the mean diameter of the pores is in the range of from about 20 to 200 μm; and a membrane of a moisture permeable poly-α-amino acid laminated to one surface of the collagen sponge base layer, said poly-α-amino acid being a copolymer of a first α-amino acid having a hydrophilic moiety with a second α-amino acid having a hydrophobic moiety, whereby said composite two-layer membrane promotes three-dimensional proliferation of fibroblasts and the normal epidermal cells surrounding the burn, cut or wound can grow and penetrate the interface between the collagen sponge base layer and the poly-α-amino acid membrane.
Claim 8
Dependent Claim
176 words
A composite two-layer membrane suitable as an artificial skin for the promotion of healing of burns, cuts or wounds, which comprises a collagen sponge base layer to be used in contact with the exposed endothelium at the site of the burn, cut or wound, said collagen sponge having an apparent density of from 0.01 to 0.05 g/cm 3 , a thickness in the range of from about 0.2 to about 0.3 mm, and a pore structure such that the mean diameter of the pores is in the range of from about 20 to 200 μm; and a membrane of a moisture permeable poly-α-amino acid laminated to one surface of the collagen sponge base layer, said poly-α-amino acid being a copolymer of a first α-amino acid having a hydrophilic moiety with a second α-amino acid having a hydrophobic moiety, whereby said composite two-layer membrane promotes three-dimensional proliferation of fibroblasts and the normal epidermal cells surrounding the burn, cut or wound can grow and penetrate the interface between the collagen sponge base layer and the poly-α-amino acid membrane.
Claim 9
Dependent Claim
12 words
The composite membrane of claim 6 wherein the collagen sponge is cross-linked.
Claim 10
Dependent Claim
34 words
The composite membrane of claim 6 wherein the collagen sponge has an apparent density of from 0.02 to 0.03 g/cm 3 and a mean pore diameter in the range of 30 to 100 μm.
Claim 11
Dependent Claim
36 words
The composite membrane of claim 7 wherein the poly-α-amino acid copolymer has the formula ##STR7## where R is ##STR8## where m is 2 to 4, and x represents the molar ratios of the glutamine amino acids.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-09 | 1990-08-11 | 1991-02-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-09 | 1994-08-11 | 1995-02-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-09 | 1998-08-11 | 1999-02-10 | $7700.00 | Window Closed / Lapsed |