Cell culture filter apparatus and method

Claim 1

Continuous cell culture filter apparatus adapted for convenient manual assembly - disassembly comprising: (A) a hollow vessel having an opening at the top, (B) a stationary, hollow shaft means positioned within said vessel in fluid communication with said top opening of said vessel and supported downwardly therefrom without any lower bearing, (C) a stationary filter unit supported downwardly from said shaft means without any lower bearing and without any moving seals between the filter unit or any moving filter support means which could contact the cell suspension, said filter having (1) a fluid collection cavity in direct communication with the interior of said shaft means and the top of said vessel, and (2) a porous outer surface having a pore size smaller than the cells to be cultured or the carrier particles upon which said cells are attached but sufficiently large to permit permeation of fluid into said fluid collection cavity, (D) a rotatable agitator means supported downwardly from said shaft means and concentrically disposed about said filter unit for rotation by suspension from bearing means having low-friction engagement with said hollow shaft means, (E) said shaft means having a boss section positioned between said filter unit and said bearing means and a spring seat adaptable for holding spring means positioned between said boss section and said filter unit, (F) said agitator means having suspended thereon a plurality of flexible vanes which are substantially equidistantly spaced apart circumferentially and which extend substantially throughout the length of said filter unit, said vanes being held between a pair of disc-like holding means at each of the upper and lower ends of the vanes and transverse to the axis of said agitator means, with the lower member of the upper pair and the upper member of the lower pair of said holding means having side slits circumferentially spaced apart to receive the widths of said vanes, and said vanes having elastomeric means at each end compressed between the corresponding pair of said holding means.