Patent US4517687A successfully refreshed from Google Patents.
US4634586A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagent and method for radioimaging leukocytes
Assignee: University of California
•
Filing: 1984-05-21
•
Granted: 1987-01-06
•
Expires: 2004-05-21
Abstract
Abstract Leukocytes are radioimmunoimaged by injecting patients with an immunoreactive nonleukocidal conjugate of an anti-leukocyte antibody and a gamma-emitting radioactive metal chelate, waiting for the conjugate to localize on the leukocytes, injecting the patient with an antibody to the conjugate to clear the blood of background nonlocalized conjugate, and visualizing the leukocytes by scintillation scanning.
Inventors
- 👤 David A. Goodwin
- 👤 Claude F. Meares
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 02:24
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; A61K51/10; A61K51/1093; A61K2123/00; Y; Y10; Y10S; Y10S424/00; Y10S424/806
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
33 words
A leukocyte radioimmunoimaging reagent comprising an immunoreactive nonleukocidal conjugate of an antibody to a leukocyte membrane determinant and a bifunctional phenyl or benzyl derivatine of EDTA or DTPA chelated with a radioactive metal.
Claim 2
Dependent Claim
33 words
A leukocyte radioimmunoimaging reagent comprising an immunoreactive nonleukocidal conjugate of an antibody to a leukocyte membrane determinant and a bifunctional phenyl or benzyl derivatine of EDTA or DTPA chelated with a radioactive metal.
Claim 3
Dependent Claim
56 words
The reagent of claim 1 wherein the radioactive metal is 99m Tc, 67 Ga, 68 Ga or 111 In and said derivative is of the formula ##STR2## where X is a functionality that reacts with an amino acid side chain of the antibody to covalently bind the chelating agent to the antibody and R is EDTA.
Claim 4
Dependent Claim
12 words
The reagent of claim 1 wherein the antibody is a monoclonal antibody.
Claim 5
Dependent Claim
13 words
The reagent of claim 1 wherein the leukocyte is a lymphocyte or granulocyte.
Claim 6
Dependent Claim
47 words
The reagent of claim 1 wherein the leukocyte is a noncancerous human lymphocyte, the antibody is a monoclonal antibody to a nontumor-specific membrane determinant of the lymphocyte, the metal is 111 In, and the bifunctional phenyl or benzyl derivative of EDTA or DTPA is 1-(p-bromoacetamidobenzyl) ethylenediaminetetraacetic acid.
Claim 7
Dependent Claim
28 words
A method for making leukocytes in a patient visible by radioimmunoimaging comprising injecting an effective amount of the reagent of claim 1 into the patient subcutaneously or intravenously.
Claim 8
Dependent Claim
25 words
A method of making leukocytes in a patient visible by radioimmunoimaging comprising injecting an effective amount of the reagent of claim 5 into the patient.
Claim 9
Dependent Claim
46 words
A method of making a site of expected lymphocyte concentration in a patient visible by radioimmunoimaging comprising injecting an effective amount of the reagent of claim 4 into the patient subcutaneously or intravenously at a location from which the reagent will migrate rapidly to the site.
Claim 10
Dependent Claim
17 words
The method of claim 8 wherein the site is a lymph node, an inflammation, or an absess.
Claim 11
Dependent Claim
87 words
A method of radioimaging leukocytes comprising injecting an effective amount of the reagent of claim 1 into the patient subcutaneously or intravenously, waiting until the conjugate localizes on the leukocytes of the patient, injecting into the patient's circulation an effective amount of antibody to the conjugate that does not cross react with endogenous components of the patient's blood, waiting for the reticuloendothial system to clear the patient's blood of complexes of the conjugate and the antibody to the conjugate, and visualizing the patient's leukocytes by scintillation scanning.
Claim 12
Dependent Claim
33 words
The method of claim 10 wherein the labeled serum protein is a leukocyte radioimmunoimaging reagent comprising an immunoreactive nonleukocidal conjugate of an antibody to a leukocyte membrane determinant and a radioactive metal chelate.
Claim 13
Dependent Claim
14 words
The method of claim 11 wherein the antibody to the conjugate is an IgM.
Claim 14
Dependent Claim
15 words
The method of claim 12 wherein the antibody to the conjugate is a monoclonal antibody.
Claim 15
Dependent Claim
22 words
The method of claim 11 wherein the antibody to the conjugate is to a determinant site on the antibody of the conjugate.
Claim 16
Dependent Claim
23 words
The method of claim 11 wherein the antibody to the conjugate is to a determinant site in the chelating agent of the conjugate.
Claim 17
Dependent Claim
63 words
A kit for use in radioimmunoimaging leukocytes in a patient comprising in: (a) a first container containing conjugate of an antibody to a nontumor-specific membrane determinant of the leukocytes and a bifunctional benzyl or phenyl derivative of EDTA or DTPA; and (b) a second container containing an antibody to the conjugate that does not cross react with endogenous components of the patient's blood.
Claim 18
Dependent Claim
63 words
A kit for use in radioimmunoimaging leukocytes in a patient comprising in: (a) a first container containing conjugate of an antibody to a nontumor-specific membrane determinant of the leukocytes and a bifunctional benzyl or phenyl derivative of EDTA or DTPA; and (b) a second container containing an antibody to the conjugate that does not cross react with endogenous components of the patient's blood.
References Cited (Backward Citations)
US4454106A
Cited
US4497791A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-01-05 | 1990-07-07 | 1991-01-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-01-05 | 1994-07-07 | 1995-01-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-01-05 | 1998-07-07 | 1999-01-06 | $7700.00 | Window Closed / Lapsed |