US4622389A
Expired / Lapsed
US
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Novel substrate for determining the activity of blood coagulation factor Xa (Stuart-Prower factor)
Assignee: Nitto Boseki Co Ltd
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Filing: 1985-05-09
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Granted: 1986-11-11
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Expires: 2005-05-09
Abstract
Abstract A novel chromogenic and fluorescent substrate for determining the activity of blood coagulation factor Xa and Xa-like enzymes. The novel substrate according to the present invention has very excellent selectivity as compared with the hitherto disclosed substrates and enables specific determination of blood coagulation factor Xa. The present substrate is therefore useful for the studies of chemical reactions involving formation, inhibition and consumption of factor Xa and can be also used for the determination of various factors associated therewith such as factor X, anti-Xa factor, factor VII, factor VIII, factor IX and heparin.
Inventors
- 👤 Takeshi Nagasawa
- 👤 Yoshio Nakamura
- 👤 Tsuyoshi Enomoto
- 👤 Katsumasa Kuroiwa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 19:55
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/56; C12Q2337/00; C12Q2337/10; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/95; G01N2333/964; G01N2333/96425; G01N2333/96427; G01N2333/9643; G01N2333/96433; G01N2333/96441; G01N2333/96444; Y; Y10; Y10S; Y10S530/00; Y10S530/802
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
153 words
A novel substrate for determining the activity of blood coagulation factor Xa (Stuart-Prower factor), said substrate having the property of developing color or becoming fluorescent when combined with said factor Xa or factor Xa-like enzymes, said substrate being composed of a compound represented by the general formula: ##STR8## [wherein n is an integer of 3-4; R 1 is a hydrogen atom or a group of the formula ##STR9## R 2 is a hydrogen atom, a methyl group or a phenyl group; R 3 is a group of the formula ##STR10## (wherein R 4 is a hydrogen atom, a methyl group, a phenyl group or a group of the formula ##STR11## and R 5 is a group of the formula ##STR12## or --C(CH 3 ) 3 , wherein R 6 is a hydrogen atom or a methoxy group)], characterized in that said compound contains 3-carboxy-4-hydroxyanilide (CHA) group in its molecule as the chromogenic group.
Claim 2
Dependent Claim
153 words
A novel substrate for determining the activity of blood coagulation factor Xa (Stuart-Prower factor), said substrate having the property of developing color or becoming fluorescent when combined with said factor Xa or factor Xa-like enzymes, said substrate being composed of a compound represented by the general formula: ##STR8## [wherein n is an integer of 3-4; R 1 is a hydrogen atom or a group of the formula ##STR9## R 2 is a hydrogen atom, a methyl group or a phenyl group; R 3 is a group of the formula ##STR10## (wherein R 4 is a hydrogen atom, a methyl group, a phenyl group or a group of the formula ##STR11## and R 5 is a group of the formula ##STR12## or --C(CH 3 ) 3 , wherein R 6 is a hydrogen atom or a methoxy group)], characterized in that said compound contains 3-carboxy-4-hydroxyanilide (CHA) group in its molecule as the chromogenic group.
References Cited (Backward Citations)
US4452736A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-11-10 | 1990-05-12 | 1990-11-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-11-10 | 1994-05-12 | 1994-11-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-11-10 | 1998-05-12 | 1998-11-11 | $7700.00 | Window Closed / Lapsed |