US4621049A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzymatic high range glucose test
Assignee: Bayer Corp
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Filing: 1984-11-19
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Granted: 1986-11-04
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Expires: 2004-11-19
Abstract
Abstract A test composition comprising glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable response and a borate buffer capable of providing an initial pH above about pH 7 is particularly useful for the semiquantitative determination of high range glucose, (i.e. glucose concentrations of 1,000 mg/dL to 10,000 mg/dL). A preferred indicator system is a water soluble iodide salt and poly(vinylpyrrolidone). The use of a borate buffer capable of providing an initial pH above about pH 7 permits greatly improved resolution for the semiquantitative determination of high range glucose when the test composition is incorported onto a carrier matrix to prepare a solid state unitary test device.
Inventors
- 👤 Joseph Y. Wang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 07:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
48 words
A test composition for the semiquantitative determination of high range glucose in an aqueous test sample, comprising; glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable colorimetric response and a borate buffer capable of providing an initial pH above about pH 7.
Claim 2
Dependent Claim
48 words
A test composition for the semiquantitative determination of high range glucose in an aqueous test sample, comprising; glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable colorimetric response and a borate buffer capable of providing an initial pH above about pH 7.
Claim 3
Dependent Claim
21 words
The test composition of claim 1 in which the borate buffer is a boric acid buffer or a phenylboronic acid buffer.
Claim 4
Dependent Claim
30 words
The test composition of claim 2 in which the borate buffer is a boric acid buffer and the chromogenic indicator system is a water soluble iodide salt and a poly(vinylpyrrolidone).
Claim 5
Dependent Claim
27 words
The test composition of claim 3 in which the boric acid buffer is capable of providing an initial pH between about pH 8.5 and about pH 9.5.
Claim 6
Dependent Claim
62 words
A test device for the semiquantitative determination of high range glucose in an aqueous fluid sample comprising: (a) a carrier matrix, and (b) a test composition incorporated therewith, the test composition including glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable response and a borate buffer capable of providing an initial pH above about pH 7.
Claim 7
Dependent Claim
62 words
A test device for the semiquantitative determination of high range glucose in an aqueous fluid sample comprising: (a) a carrier matrix, and (b) a test composition incorporated therewith, the test composition including glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable response and a borate buffer capable of providing an initial pH above about pH 7.
Claim 8
Dependent Claim
21 words
The test device of claim 5 in which the borate buffer is a boric acid buffer or a phenylboronic acid buffer.
Claim 9
Dependent Claim
29 words
The test device of claim 6 in which the borate buffer is a boric acid buffer and the chromogenic indicator system is a water soluble iodide salt and poly(vinylpyrrolidone).
Claim 10
Dependent Claim
28 words
The test device of claim 7 in which the boric acid buffer is capable of providing an initial pH is between about pH 8.5 and about pH 9.5.
Claim 11
Independent Claim
74 words
A method of preparing the test device for the semiquantitative determination of high range glucose in an aqueous test sample, comprising the steps of: (a) incorporating with a carrier matrix with a test composition including glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable response and from about 0.2 M to about 0.8 M borate buffer capable of providing an initial pH above about 7; and (b) drying.
Claim 12
Independent Claim
74 words
A method of preparing the test device for the semiquantitative determination of high range glucose in an aqueous test sample, comprising the steps of: (a) incorporating with a carrier matrix with a test composition including glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable response and from about 0.2 M to about 0.8 M borate buffer capable of providing an initial pH above about 7; and (b) drying.
Claim 13
Dependent Claim
29 words
The method of claim 9 in which the borate buffer is a boric acid buffer and the chromogenic indicator system is a water soluble iodide salt and a poly(vinylpyrrolidone).
Claim 14
Dependent Claim
26 words
The method of claim 10 in which the boric acid buffer is capable of providing an initial pH between about pH 8.5 and about pH 9.5.
Claim 15
Dependent Claim
43 words
The method of claim 9 in which the carrier matrix is paper and the method includes the additional step of pretreating the paper matrix with a borate buffer at an initial pH about 7 prior to incorporating the matrix with the test composition.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-11-03 | 1990-05-05 | 1990-11-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-11-03 | 1994-05-05 | 1994-11-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-11-03 | 1998-05-05 | 1998-11-04 | $7700.00 | Window Closed / Lapsed |