US4619795A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for preparing lipid vesicles
Assignee: Bayer Corp
•
Filing: 1984-12-24
•
Granted: 1986-10-28
•
Expires: 2004-12-24
Abstract
Abstract A method for forming vesicles is described, wherein a lipid coating is formed onto a deformable surface. During hydration, the lipid coating is fragmented by deformation of the surface. The resulting fragments of the lipid coating are dispersed in the hydrating medium, so as to be subjected to multidirectional hydration, whereby formation of vesicles is accelerated. In the preferred embodiment, such surface is defined by a plurality of water-swellable particles formed of a polymeric material.
Inventors
- 👤 Beri Cohen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:21
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1277; Y; Y10; Y10S; Y10S436/00; Y10S436/829; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2984
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
85 words
A method of preparing vesicles, which method comprises: a. depositing a coating of lipid onto the surface of one or more particles, said particles being deformable in a hydrating aqueous phase, to form a coated particle; and b. deforming said coated particle by contacting it with a hydrating aqueous phase in order to fragment said lipid coating so as to disperse fragments of said lipid into said aqueous phase and subject said fragments to multidirectional hydration, whereby lipid vesicles are formed in said aqueous phase.
Claim 2
Independent Claim
85 words
A method of preparing vesicles, which method comprises: a. depositing a coating of lipid onto the surface of one or more particles, said particles being deformable in a hydrating aqueous phase, to form a coated particle; and b. deforming said coated particle by contacting it with a hydrating aqueous phase in order to fragment said lipid coating so as to disperse fragments of said lipid into said aqueous phase and subject said fragments to multidirectional hydration, whereby lipid vesicles are formed in said aqueous phase.
Claim 3
Dependent Claim
58 words
The method of claim 1, wherein said depositing step comprises forming an organic phase comprising lipid and an organic solvent, contacting said deformable particle with said organic phase, and evaporating said organic solvent, said deformable particle being formed of a material which is sufficiently hydrophobic when exposed to said organic phase to permit deposition of said lipid coating.
Claim 4
Dependent Claim
29 words
The method of claim 1, wherein said depositing step comprises forming an organic phase comprising lipids in a fluid state, and contacting said deformable particle with said organic phase.
Claim 5
Dependent Claim
28 words
The method of claim 2 or 3, comprising the additional step of separating said coated particles from components of said organic phase which have not coated said particle.
Claim 6
Dependent Claim
14 words
The method of claim 2 or 3, wherein said organic phase comprises a phospholipid.
Claim 7
Dependent Claim
14 words
The method of claim 2 or 3, wherein said organic phase further comprises cholesterol.
Claim 8
Dependent Claim
14 words
The method of claim 2 or 3, wherein said organic phase further comprises cholestanol.
Claim 9
Dependent Claim
16 words
The method of claim 2 or 3, wherein said organic phase further comprises a ligand-lipid conjugate.
Claim 10
Dependent Claim
19 words
The method of claim 2 or 3, wherein said organic phase further comprises at least one therapeutically effective substance.
Claim 11
Dependent Claim
44 words
The method of claim 1, further comprising the step of introducing a marker into said aqueous phase prior to contacting said aqueous phase with said coated particle, said marker being selected from the group consisting of enzymes, substrates, fluorphors, spin-label markers, luminophors and radioisotopes.
Claim 12
Dependent Claim
26 words
The method of claim 1, further comprising the step of introducing a therapeutically effective drug into said aqueous phase prior to contacting with said coated particle.
Claim 13
Dependent Claim
22 words
The method of claim 1, wherein said deformable particle is formed of a material which swells when exposed to said aqueous phase.
Claim 14
Dependent Claim
23 words
The method of claim 1, wherein said depositing step comprises depositing said lipid coating onto the surface of one or more water-swellable particles.
Claim 15
Dependent Claim
23 words
The method of claim 13, wherein said depositing step comprises depositing said lipid coating onto the surface of one or more polymeric particles.
Claim 16
Dependent Claim
35 words
The method of claim 14, wherein said depositing step comprises depositing said lipid coating onto the surface of one or more particles formed of a material selected from the group consisting of polyamides, and polysaccharides.
References Cited (Backward Citations)
US4247411A
Cited
US4186183A
Cited
US4280816A
Cited
US4342826A
Cited
US4480041A
Cited
US4485054A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-10-27 | 1990-04-28 | 1990-10-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-10-27 | 1994-04-28 | 1994-10-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-10-27 | 1998-04-28 | 1998-10-28 | $7700.00 | Window Closed / Lapsed |