Hybridoma cell lines producing monoclonal antibodies directed against cervical cancer cells
Abstract
Abstract Continuous hybrid cell lines for producing monoclonal antibodies, the antibodies specific for an antigenic determinant unique to cervical cancer cells, have been developed. The hybrid cell lines were established by fusing differentiated lymphoid cells primed with intact human cervical cancer cells with myeloma cells, particularly plasmacytoma cells. The resulting fused hybrid cells were cultured in HAT tissue culture media which included a small concentration of deoxycytidine. Deoxycytidine was found to enhance the growth of the hybrid cells and subsequent yield of monoclonal antibodies secreted therefrom. Hybrid cell lines secreting monoclonal antibodies to antigenic determinants unique for human cervical cancer cells can be maintained indefinitely in culture to produce large amounts of homogenous anti-cervical cancer cell antibody.
Inventors
- 👤 Teh-sheng Chan
Bibliographic Details
Claims Analysis
Total Claims: 4References Cited (Backward Citations)
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