US4618582A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing Xanthomonas biopolymers
Assignee: Henkel AG and Co KGaA
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Filing: 1982-02-08
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Granted: 1986-10-21
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Expires: 2002-02-08
Abstract
Abstract In the aerobic culture of microorganisms of the Xanthomonas genus in fermentation media, the use of water-in-oil emulsions in the media minimizes viscosity problems and enhances yields of Xanthomonas biopolymers. Preferably, the emulsion is formed with a surfactant and the microorganism is Xanthomonas campestris.
Inventors
- 👤 Konrad Engelskirchen
- 👤 Werner Stein
- 👤 Michael Bahn
- 👤 Ludwig Schieferstein
- 👤 Joachim Schindler
- 👤 Rolf Schmid
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 16:12
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/04; C12P19/06; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/91
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
82 words
In the method of conducting a fermentation reaction wherein an aqueous culture medium comprising a carbohydrate source and a nitrogen source is inoculated with a polysaccharide gum producing Xanthomonas microorganism and said medium is mechanically agitated and aerated under conditions to effect fermentation thereof, the improvement which comprises said culture medium being dispersed via a surfactant in about 15 to about 90%, based on the total weight of dispersion, of a water insoluble oil in which the resultant polysaccharide is also insoluble.
Claim 2
Dependent Claim
82 words
In the method of conducting a fermentation reaction wherein an aqueous culture medium comprising a carbohydrate source and a nitrogen source is inoculated with a polysaccharide gum producing Xanthomonas microorganism and said medium is mechanically agitated and aerated under conditions to effect fermentation thereof, the improvement which comprises said culture medium being dispersed via a surfactant in about 15 to about 90%, based on the total weight of dispersion, of a water insoluble oil in which the resultant polysaccharide is also insoluble.
Claim 3
Dependent Claim
18 words
The method of claim 1, wherein the water insoluble oil is a hydrocarbon or a mixture of hydrocarbons.
Claim 4
Dependent Claim
14 words
The method of claim 1, wherein the water insoluble oil is a mineral oil.
Claim 5
Dependent Claim
21 words
The method of claim 1, wherein from about 20 to about 60% by weight of a water insoluble oil is present.
Claim 6
Dependent Claim
21 words
The method of claim 1, wherein from about 25 to about 50% by weight of a water insoluble oil is present.
Claim 7
Dependent Claim
26 words
The method of claim 1, wherein the surfactant is present in from about 0.1 to about 10% by weight based on the total weight of dispersion.
Claim 8
Dependent Claim
18 words
The method of claim 6, wherein from about 1 to about 5% by weight of surfactant is present.
Claim 9
Dependent Claim
21 words
The method of claim 1, wherein the water insoluble oil has a melting point that does not exceed about 25° C.
Claim 10
Dependent Claim
49 words
The method of claim 1, wherein the Xanthomonas microorganism is first grown for a period of time in an aqueous culture medium, followed by the addition of the surfactant and the water insoluble oil to form a water -in- oil dispersion, and the growth of the microorganism is continued.
Claim 11
Dependent Claim
11 words
The method of claim 1, wherein the microorganism is Xanthomonas campestris.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-10-20 | 1990-04-21 | 1990-10-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-10-20 | 1994-04-21 | 1994-10-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-10-20 | 1998-04-21 | 1998-10-21 | $7700.00 | Window Closed / Lapsed |