Process for the production of modified proteins and product thereof

Claim 2

A process for chemically altering the substrate specificity of a cofactor containing native holoprotein to produce a predetermined cofactorless ensyme-like modified protein comprising the steps of: selecting an enzyme to be modeled, said enzyme having an enzymatic activity different from said native holoprotein; partially denaturing a cofactor containing native holoprotein by removing the cofactor therefrom by contacting the holoprotein with a cofactor removal agent to produce a partially denatured apoprotein; cross-linking said partially denatured apoprotein in the presence of a competitive inhibitor of said model enzyme with a cross-linking agent to produce an enzyme-like modified protein having the catalytic activity of said model enzyme.

Claim 3

The product of the process of claim 1.

Claim 4

A process for chemically altering the substrate specificity of a cofactor containing native holoprotein to produce a predetermined enzyme-like modified protein comprising the steps of: selecting an enzyme to be modeled, said enzyme having an enzymatic activity different from said native holoprotein; partially denaturing a cofactor containing native holoprotein by removing the cofactor therefrom by contacting the holoprotein with a cofactor removal agent to produce a partially denatured apoprotein; contacting said partially denatured apoprotein with a competitive inhibitor of said selected model enzyme to form a partially denatured apoprotein-inhibitor complex; contacting said partially denatured apoprotein-inhibitor complex with a solid support for a time sufficient and at a temperature sufficient to absorb and immobilize said partially denatured apoprotein-inhibitor complex on said solid support; and cross-linking said absorbed, immobilized partially denatured apoprotein-inhibitor complex with a cross-linking agent to produce an enzyme-like modified protein having the catalytic activity of said model enzyme.

Claim 5

A process for chemically altering the substrate specificity of a cofactor containing native holoprotein to produce a predetermined enzyme-like modified protein comprising the steps of: selecting an enzyme to be modeled, said enzyme having an enzymatic activity different from said native holoprotein; partially denaturing a cofactor containing native holoprotein by removing the cofactor therefrom by contacting the holoprotein with a cofactor removal agent to produce a partially denatured apoprotein; contacting said partially denatured apoprotein with a competitive inhibitor of said selected model enzyme to form a partially denatured apoprotein-inhibitor complex; contacting said partially denatured apoprotein-inhibitor complex with a solid support for a time sufficient and at a temperature sufficient to absorb and immobilize said partially denatured apoprotein-inhibitor complex on said solid support; and cross-linking said absorbed, immobilized partially denatured apoprotein-inhibitor complex with a cross-linking agent to produce an enzyme-like modified protein having the catalytic activity of said model enzyme.

Claim 6

The process defined in claim 3 wherein said solid support is a ceramic oxide.

Claim 7

The process defined in claim 3 wherein said solid support is an organic polymer support.

Claim 8

The product of the process of claim 3.

Claim 9

A process for chemically altering the substrate specificity of a cofactor containing native holoprotein to produce a predetermined enzyme-like modified protein comprising the steps of: selecting an enzyme to be modeled, said enzyme having an enzymatic activity different from said native holoprotein; absorbing a native holoprotein onto a solid support to immobilize said native holoprotein on said solid support; partially denaturing said immobilized holoprotein by removing the cofactor therefrom by contacting the holoprotein with a cofactor removal agent to produce a partially denatured, immobilized apoprotein; contacting said partially denatured, immobilized apoprotein with a competitive inhibitor of said selected model enzyme; and cross-linking said partially denatured immobilized apoprotein in the presence of said inhibitor of said model enzyme with a cross-linking agent to produce an enzyme-like modified protein having the catalytic activity of said model enzyme.

Claim 10

A process for chemically altering the substrate specificity of a cofactor containing native holoprotein to produce a predetermined enzyme-like modified protein comprising the steps of: selecting an enzyme to be modeled, said enzyme having an enzymatic activity different from said native holoprotein; absorbing a native holoprotein onto a solid support to immobilize said native holoprotein on said solid support; partially denaturing said immobilized holoprotein by removing the cofactor therefrom by contacting the holoprotein with a cofactor removal agent to produce a partially denatured, immobilized apoprotein; contacting said partially denatured, immobilized apoprotein with a competitive inhibitor of said selected model enzyme; and cross-linking said partially denatured immobilized apoprotein in the presence of said inhibitor of said model enzyme with a cross-linking agent to produce an enzyme-like modified protein having the catalytic activity of said model enzyme.

Claim 11

The process claimed in claim 7 wherein said solid support is a ceramic oxide.

Claim 12

The process claimed in claim 7 wherein said solid support is an organic polymer support.

Claim 13

The product of the process of claim 7.

Claim 14

A process for chemically altering the substrate specificity of a cofactor containing native holoprotein to produce a predetermined enzyme-like modified protein comprising the steps of: selecting an enzyme to be modeled, said enzyme having an enzymatic activity different from said native holoprotein; immobilizing a competitive inhibitor of said model enzyme on a solid support; partially denaturing a cofactor containing native holoprotein by removing the cofactor therefrom by contacting the holoprotein with a cofactor removal agent to produce a partially denatured apoprotein; contacting said partially denatured apoprotein with said immobilized inhibitor to form a partially denatured apoprotein-immobilized inhibitor complex; and cross-linking said partially denatured apoprotein while in contact with said immobilized inhibitor with a cross-linking agent to produce an enzyme-like modified protein having the biological activity of said model enzyme.

Claim 15

A process for chemically altering the substrate specificity of a cofactor containing native holoprotein to produce a predetermined enzyme-like modified protein comprising the steps of: selecting an enzyme to be modeled, said enzyme having an enzymatic activity different from said native holoprotein; immobilizing a competitive inhibitor of said model enzyme on a solid support; partially denaturing a cofactor containing native holoprotein by removing the cofactor therefrom by contacting the holoprotein with a cofactor removal agent to produce a partially denatured apoprotein; contacting said partially denatured apoprotein with said immobilized inhibitor to form a partially denatured apoprotein-immobilized inhibitor complex; and cross-linking said partially denatured apoprotein while in contact with said immobilized inhibitor with a cross-linking agent to produce an enzyme-like modified protein having the biological activity of said model enzyme.

Claim 16

The process of claim 11 wherein said support is a ceramic oxide support.

Claim 17

The process of claim 11 wherein said support is an organic polymer support.

Claim 18

The product of the process of claim 11.

Claim 19

The process defined in claim 1 wherein said native holoprotein is selected from the group consisting of concanavalin A, glucose oxidase, and tryptophanase, said enzyme to be modeled is selected from the group consisting of glucose isomerase, esterase, and aldose-1 epimerase, and said inhibitor is selected from the group consisting of cellobiose, mannitol, and indole.

Claim 20

The process defined in claim 3 wherein said native holoprotein is selected from the group consisting of concanavalin A, glucose oxidase, and tryptophanase, said enzyme to be modeled is selected from the group consisting of glucose isomerase, esterase, and aldose-1 epimerase, and said inhibitor is selected from the group consisting of cellobiose, mannitol, and indole.

Claim 21

The process defined in claim 7 wherein said native holoprotein is selected from the group consisting of concanavalin A, glucose oxidase, and tryptophanase, said enzyme to be modeled is selected from the group consisting of glucose isomerase, esterase, and aldose-1 epimerase, and said inhibitor is selected from the group consisting of cellobiose, mannitol, and indole.

Claim 22

The process defined in claim 11 wherein said native holoprotein is selected from the group consiting of concanavalin A, glucose oxidase, and tryptophanase, said enzyme to be modeled is selected from the group consisting of glucose isomerase, esterase, and aldose-1 epimerase, and said inhibitor is selected from the group consisting of cellobiose, mannitol, and indole.