US4608341A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Living, fast-growing thyroid cell strain, FRTL-5
Assignee: Interthyr Research Foundation Inc
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Filing: 1983-05-31
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Granted: 1986-08-26
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Expires: 2003-05-31
Abstract
Abstract A fast growing, continuous, functional rat thyroid cell strain, FRTL-5, which maintains functional characteristics of iodide uptake and thyroglobulin synthesis over prolonged periods of culture is cloned from FRTL cells obtained from primary cultures of Fischer rat thyroid glands. The FRTL-5 cells are cultured in a medium containing approximately 5 percent calf serum supplemented with a mixture of hormones, at least one of which is thyrotropin. The FRTL-5 cells are employed in a series of assays which measure thyroid stimulatory or inhibitory factors. The FRTL-5 system of assay specifically measures thymidine incorporation, cAMP elevation and iodide uptake and permits the evaluation of patient sera, particularly those afflicted with Graves' disease and other autoimmune thyroid diseases, thereby providing a means for determining appropriate methods of treatment.
Inventors
- 👤 Francesco S. Ambesi-Impiombato
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:14
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0613; C12N5/0617; G01N33/53; G01N33/564; C12N2500/00; C12N2500/05; C12N2500/10; C12N2500/20; C12N2500/24; C12N2500/25; C12N2501/00; C12N2501/10; C12N2501/18; C12N2503/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
65 words
A process for preparing a continuous, functional rat cell strain, having the identifying characteristics of FRTL-5 ATCC CRL 8305 cell strain, comprising: culturing a continuous, functional rat cell line FRTL in a medium comprising thyrotropin and calf serum to obtain FRTL-5 cells and cloning said FRTL-5 cells to obtain cells of an FRTL-5 strain having the identifying characteristics of FRTL-5 ATCC CRL 8305 cell strain.
Claim 2
Dependent Claim
65 words
A process for preparing a continuous, functional rat cell strain, having the identifying characteristics of FRTL-5 ATCC CRL 8305 cell strain, comprising: culturing a continuous, functional rat cell line FRTL in a medium comprising thyrotropin and calf serum to obtain FRTL-5 cells and cloning said FRTL-5 cells to obtain cells of an FRTL-5 strain having the identifying characteristics of FRTL-5 ATCC CRL 8305 cell strain.
Claim 3
Dependent Claim
24 words
The process for preparing a continuous, functional rat cell strain, FRTL-5, according to claim 1 wherein said medium is Coon's modified Ham's F-12 medium.
Claim 4
Dependent Claim
25 words
The process for preparing a continuous, functional rat cell strain, FRTL-5, according to claim 1 wherein said medium is supplemented with a mixture of hormones.
Claim 5
Dependent Claim
28 words
The process for preparing a continuous, functional rat cell strain, FRTL-5, according to claim 3 wherein said mixture of hormones comprises insulin, hydrocortisone, transferrin, glycyl-L-histidyl-L-lysine acetate, and somatostatin.
Claim 6
Dependent Claim
23 words
The process for preparing a continuous, functional rat cell strain, FRTL-5 according to claim 1 wherein the cell line is cultured in vitro.
Claim 7
Dependent Claim
39 words
The process for preparing a continuous, functional rat cell strain, FRTL-5, according to claim 1 wherein said calf serum is present in the range of about 2.5 to 15 percent, by volume, based on the volume of the medium.
Claim 8
Dependent Claim
37 words
The process for preparing a continuous, functional rat cell strain, FRTL-5, according to claim 6 wherein said calf serum is present in an amount of about 5 percent, by volume, based on the volume of the medium.
Claim 9
Dependent Claim
29 words
The process for preparing a continuous, functional rat cell strain FRTL-5 according to claim 1 wherein FRTL-5 cells are cloned to obtain an FRTL-5 ATCC CRL 8305 cell strain.
Claim 10
Dependent Claim
15 words
A continuous, functional rat thyroid cell strain, FRTL-5, having the accession number ATCC CRL 8305.
Claim 11
Dependent Claim
15 words
A continuous, functional rat thyroid cell strain, FRTL-5, having the accession number ATCC CRL 8305.
Claim 12
Dependent Claim
18 words
A continuous, functional rat thyroid cell strain having the identifying characteristics of FRTL-5 ATCC CRL 8305 cell strain.
Claim 13
Dependent Claim
18 words
A continuous, functional rat thyroid cell strain having the identifying characteristics of FRTL-5 ATCC CRL 8305 cell strain.
References Cited (Backward Citations)
No citation records indexed.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-08-25 | 1990-02-24 | 1990-08-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-08-25 | 1994-02-24 | 1994-08-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-08-25 | 1998-02-24 | 1998-08-26 | $7700.00 | Window Closed / Lapsed |