US4606825A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purification of immunoglobulin G
Assignee: Avantor Performance Materials LLC
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Filing: 1985-04-22
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Granted: 1986-08-19
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Expires: 2005-04-22
Abstract
Abstract A method of separating and recovering immunoglobulin G from biological fluids by liquid chromatographic or solid phase extraction processes utilizing, as the solid phase, a carboxylated form of silica gel or controlled pore glass bearing non-cross-linked covalently bound polyethylenimine functions.
Inventors
- 👤 Laura J. Crane
- 👤 David R. Nau
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:16
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/395; C; C07; C07K; C07K16/00; C07K16/06; C07K16/065
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
136 words
A method of obtaining substantially purified immunoglobulin G from a sample of biological fluid containing said immunoglobulin G which comprises separating and recovering said immunoglobulin G from said sample by employing liquid chromatography or solid phase extraction wherein the chromatographic packing or solid phase matrix, respectively, consists essentially of the carboxylated covalently bound, non-crosslinked polyethylenimine reaction product of particulate silica gel having an average particle diameter of from about 3 to about 70 microns and an average pore size of from about 50 to about 1000 Angstrom units, or particulate controlled pore glass having an average particle diameter of from about 37 to about 177 microns and an average pore size of from about 40 to about 1000 Angstrom units, with polyethyleniminopropyl trimethoxy silane having an average molecular weight of from about 400 to about 1800.
Claim 2
Independent Claim
136 words
A method of obtaining substantially purified immunoglobulin G from a sample of biological fluid containing said immunoglobulin G which comprises separating and recovering said immunoglobulin G from said sample by employing liquid chromatography or solid phase extraction wherein the chromatographic packing or solid phase matrix, respectively, consists essentially of the carboxylated covalently bound, non-crosslinked polyethylenimine reaction product of particulate silica gel having an average particle diameter of from about 3 to about 70 microns and an average pore size of from about 50 to about 1000 Angstrom units, or particulate controlled pore glass having an average particle diameter of from about 37 to about 177 microns and an average pore size of from about 40 to about 1000 Angstrom units, with polyethyleniminopropyl trimethoxy silane having an average molecular weight of from about 400 to about 1800.
Claim 3
Dependent Claim
25 words
In the method of claim 1, said carboxylated reaction product having a carboxyl milliequivalent per gram of product of from about 0.3 to about 1.2.
Claim 4
Dependent Claim
18 words
In the method of claim 2, said biological fluid being plasma, serum, tissue culture supernatant or ascites fluid.
Claim 5
Independent Claim
104 words
A method of obtaining substantially purified immunoglobulin G from a sample of biological fluid containing said immunoglobulin G which comprises separating and recovering said immunoglobulin G from said sample by employing liquid chromatography or solid phase extraction wherein the chromatographic packing or solid phase matrix, respectively, consists essentially of the carboxylated covalently bound, non-crosslinked polyethylenimine reaction product of particulate silica gel having an average particle diameter of from about 3 to about 70 microns and an average pore size of from about 50 to about 1000 Angstrom units with polyethyleniminopropyl trimethoxy silane having an average molecular weight of from about 400 to about 1800.
Claim 6
Independent Claim
104 words
A method of obtaining substantially purified immunoglobulin G from a sample of biological fluid containing said immunoglobulin G which comprises separating and recovering said immunoglobulin G from said sample by employing liquid chromatography or solid phase extraction wherein the chromatographic packing or solid phase matrix, respectively, consists essentially of the carboxylated covalently bound, non-crosslinked polyethylenimine reaction product of particulate silica gel having an average particle diameter of from about 3 to about 70 microns and an average pore size of from about 50 to about 1000 Angstrom units with polyethyleniminopropyl trimethoxy silane having an average molecular weight of from about 400 to about 1800.
Claim 7
Dependent Claim
25 words
In the method of claim 4, said carboxylated reaction product having a carboxyl milliequivalent per gram of product of from about 0.3 to about 1.2.
Claim 8
Dependent Claim
18 words
In the method of claim 5, said biological fluid being plasma, serum, tissue culture supernatent or ascites fluid.
Claim 9
Independent Claim
105 words
A method of obtaining substantially purified immunoglobulin G from a sample of biological fluid containing said immunoglobulin G which comprises separating and recovering said immunoglobulin G from said sample by employing liquid chromatography or solid phase extraction wherein the chromatographic packing or solid phase matrix, respectively, consists essentially of the carboxylated covalently bound, non-crosslinked polyethylenimine reaction product of particulate controlled pore glass having an average particle diameter of from about 37 to about 177 microns and an average pore size of from about 40 to about 1000 Angstrom units, with polyethyleniminopropyl trimethoxy silane having an average molecular weight of from about 400 to about 1800.
Claim 10
Independent Claim
105 words
A method of obtaining substantially purified immunoglobulin G from a sample of biological fluid containing said immunoglobulin G which comprises separating and recovering said immunoglobulin G from said sample by employing liquid chromatography or solid phase extraction wherein the chromatographic packing or solid phase matrix, respectively, consists essentially of the carboxylated covalently bound, non-crosslinked polyethylenimine reaction product of particulate controlled pore glass having an average particle diameter of from about 37 to about 177 microns and an average pore size of from about 40 to about 1000 Angstrom units, with polyethyleniminopropyl trimethoxy silane having an average molecular weight of from about 400 to about 1800.
Claim 11
Dependent Claim
25 words
In the method of claim 7, said carboxylated reaction product having a carboxyl milliequivalent per gram of product of from about 0.3 to about 1.2.
Claim 12
Dependent Claim
18 words
In the method of claim 8, said biological fluid being plasma, serum, tissue culture supernatent or ascites fluid.
Claim 13
Independent Claim
177 words
A method of obtaining substantially purified immunoglobulin G from a sample of biological fluid containing said immunoglobulin G which comprises: a. equilibrating said sample of biological fluid to an ionic strength equal to or less than the ionic strength of the lower ionic strength buffer used for gradient elution in the subsequent chromatographic separation and recovery step and to a pH greater than the pI of the particular immunoglobulin G, and b. separating and recovering said immunoglobulin G from said sample by employing liquid chromatography wherein the chromatographic packing consists essentially of the carboxylated covalently bound, non-crosslinked reaction product of particulate silica gel having an average particle diameter of from about 3 to about 70 microns and average pore size of from about 50 to about 1000 Angstrom units, or particulate controlled pore glass having an average particle diameter of from about 37 to about 177 microns and an average pore size of from about 40 to about 1000 Angstrom units, with polyethyleniminopropyl trimethoxy silane having an average molecular weight of from about 400 to about 1800.
Claim 14
Independent Claim
177 words
A method of obtaining substantially purified immunoglobulin G from a sample of biological fluid containing said immunoglobulin G which comprises: a. equilibrating said sample of biological fluid to an ionic strength equal to or less than the ionic strength of the lower ionic strength buffer used for gradient elution in the subsequent chromatographic separation and recovery step and to a pH greater than the pI of the particular immunoglobulin G, and b. separating and recovering said immunoglobulin G from said sample by employing liquid chromatography wherein the chromatographic packing consists essentially of the carboxylated covalently bound, non-crosslinked reaction product of particulate silica gel having an average particle diameter of from about 3 to about 70 microns and average pore size of from about 50 to about 1000 Angstrom units, or particulate controlled pore glass having an average particle diameter of from about 37 to about 177 microns and an average pore size of from about 40 to about 1000 Angstrom units, with polyethyleniminopropyl trimethoxy silane having an average molecular weight of from about 400 to about 1800.
Claim 15
Dependent Claim
25 words
In the method of claim 10, said carboxylated reaction product having a carboxyl milliequivalent per gram of product of from about 0.3 to about 1.2.
Claim 16
Dependent Claim
18 words
In the method of claim 11, said biological fluid being plasma, serum, tissue culture supernatent or ascites fluid.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-08-18 | 1990-02-17 | 1990-08-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-08-18 | 1994-02-17 | 1994-08-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-08-18 | 1998-02-17 | 1998-08-19 | $7700.00 | Window Closed / Lapsed |